Evaluation of verification assays in EIA specimens presumptively positive for Free

Abstract

Verification of specimens positive for by enzyme immunoassay (EIA) has been recommended when testing low prevalence populations. This study compared direct fluorescent antibody (DFA) and blocking antibody (BLA) verification assays in specimens presumptively positive for by the Syva Microtrak II EIA. Of 1785 specimens originally tested by EIA, 96 were presumptively positive for Verification assays were concordant in 86 specimens (69 positive, 17 negative); nine of the remaining samples gave positive results in a second EIA and one was unresolved. Both verification assays gave some false-negative results. When initial EIA absorbance values were correlated with verified results, all EIA false positive results had absorbances in the low range (less than a three-fold increase over assay cutoff values). Verification of EIA results by both DFA and BLA was effective in detecting false positive results, but confining verification to low-value positive specimens could be considered for cost-effective testing.

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1996-02-01
2024-03-29
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References

  1. Centers for Disease Control and Prevention Recommendations for the prevention and management of Chlamydia trachomatis infections, 1993. MMWR 1993; 42: (No. RR-12)
    [Google Scholar]
  2. Moncada J., Schachter J., Bolan G. Confirmatory assay increases specificity of the Chlamydiazyme test for Chlamydia trachomatis infection of the cervix. J Clin Microbiol 1990; 28:1770–1773
    [Google Scholar]
  3. Kellogg J. A., Seiple J. W., Murray C. L., Levisky J. S. Effect of endocervical specimen quality on detection of Chlamydia trachomatis and on the incidence of false-positive results with the Chlamydiazyme method. J Clin Microbiol 1990; 28:1105–1113
    [Google Scholar]
  4. Schwebke J. R., Stamm W. E., Handsfield H. Use of sequential enzyme immunoassay and direct fluorescent antibody tests for detection of Chlamydia trachomatis infections in women. J Clin Microbiol 1990; 28:2473–2476
    [Google Scholar]
  5. Leonardi G. P., Seitz M., Edstrom R., Cruz J., Costello P., Szabo K. Evaluation of three immunoassays for detection of Chlamydia trachomatis in urine specimens from asymptomatic males. J Clin Microbiol 1992; 30:2793–2796
    [Google Scholar]
  6. Chan E. L., Brandt K., Horsman G. B. A. 1-year evaluation of Syva MicroTrak chlamydia enzyme immunoassay with selective confirmation by direct fluorescent antibody assay in a high-volume laboratory. J Clin Microbiol 1994; 32:2208–2211
    [Google Scholar]
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