Fatty acid modifying enzyme (FAME) is an extracellular enzyme that inactivates staphylocidal lipids by catalysing the esterification of these lipids to cholesterol. In-vitro expression of FAME began at the start of the stationary phase. This expression of FAME was very similar to other staphylococcal extracellular proteins controlled by the global regulators Agr and Sar. A strain ISP546 (Agr) produced 80% less FAME than an isogenic Agr strain ISP479C. Similar results were obtained with the isogenic Agr/Agr strain pair RN6390 and RN6911. A strain R (Sar) produced 86% less FAME than an isogenic Sar strain RN6390. However, lipase assays on the same culture filtrates from the Sar/Sar strains did not demonstrate any affect on lipase production by the mutation.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error