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Volume 44, Issue 1

Molecular typing of subsp. serovar Enteritidis isolates Free

Abstract

A collection of 31 epidemiologically unrelated subsp. serovar Enteritidis ( Enteritidis) isolates obtained during a 12-year period was characterised by different molecular typing methods. Plasmid profile analysis, the detection of plasmid-encoded virulence genes and ribotyping allowed little or no further differentiation amongst these isolates. Two different hybridisation patterns were observed by IS-typing of the Enteritidis isolates. However, pulsed-field gel electrophoretic separation of restriction endonuclease-digested whole-cell DNA provided a high level of discrimination amongst the 31 Enteritidis isolates. This could be increased by the comparative use of the three suitable restriction endonucleases I, I and I. Thus, pulsed-field gel electrophoresis proved to be superior in its discriminatory value to other molecular methods such as plasmid analysis, ribotyping or IS-typing and represents a most helpful tool for the epidemiological typing of Enteritidis isolates.

  • Received:
  • Accepted:
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© J. MED. MICROBIOL. 1996
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1996-01-01
2024-04-19
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Molecular typing of Salmonella enterica subsp. enterica serovar Enteritidis isolates
J. Med. Microbiol. 44, 52 (1996); https://doi.org/10.1099/00222615-44-1-52
/content/journal/jmm/10.1099/00222615-44-1-52
/content/journal/jmm/10.1099/00222615-44-1-52
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