1887

Abstract

Summary

Specific regions of adherence binding sites and epitopes of the P1 adhesin of were synthesised as octapeptides and used as targets in a modified enzyme-linked immunosorbent assay. Acute phase and convalescent sera from 10 patients with infection were tested for antibody reactivity to these octapeptides. In convalescent sera, antibody activities were directed against octapeptides of the epitope regions, whereas no antibody activity was found in acute or convalescent sera to octapeptides of adherence-mediating binding sites. The non-responsiveness to adherence-mediating binding sites could be explained partially from the results of cross-reactivity experiments with adherence-inhibiting anti-Pi adhesin monoclonal antibodies (MAbs). Two of these MAbs showed cross-reactions with intracellular antigens of eukaryotic cell lines in immunofluorescence microscopy experiments. The cross-reacting antigens were isolated and characterised as glyceraldehyde-3-phosphate dehydrogenase and 2-phospho-D-glycerate hydrolase. Antigenic mimicry of eukaryotic structures by functional sites of the P1 adhesin of may influence the pathogenesis of infection.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-43-6-422
1995-12-01
2023-02-06
Loading full text...

Full text loading...

/deliver/fulltext/jmm/43/6/medmicro-43-6-422.html?itemId=/content/journal/jmm/10.1099/00222615-43-6-422&mimeType=html&fmt=ahah

References

  1. Marrie T.J. Community-acquired pneumonia. Clin Infect Dis 1994; 18:501–515
    [Google Scholar]
  2. Razin S., Jacobs E. Mycoplasma adhesion. J Gen Microbiol 1992; 138:407–422
    [Google Scholar]
  3. Gerstenecker B., Jacobs E. Topological mapping of the Pl-adhesin of Mycoplasma pneumoniae with adherence-inhibiting monoclonal antibodies. J Gen Microbiol 1990; 136:471–476
    [Google Scholar]
  4. Jacobs E., Pilatschek A., Gerstenecker B., Oberle K., Bredt W. Immunodominant epitopes of the adhesin of Mycoplasma pneumoniae. J Clin Microbiol 1990; 28:1194–1197
    [Google Scholar]
  5. Jacobs E., Fuchte K., Bredt W. Amino acid sequence and antigenicity of the amino-terminus of the 168 kDa adherence protein of Mycoplasma pneumoniae. J Gen Microbiol 1987; 133:2233–2236
    [Google Scholar]
  6. Jacobs E. Mycoplasma pneumoniae virulence factors and the immune response. Rev Med Microbiol 1991; 2:83–90
    [Google Scholar]
  7. Kenny G.E., Kaiser G.G., Cooney M.K., Foy H.M. Diagnosis of Mycoplasma pneumoniae pneumonia: sensitivities and specificities of serology with lipid antigen and isolation of the organism on soy peptone medium for identification of infections. J Clin Microbiol 1990; 28:2087–2093
    [Google Scholar]
  8. Jacobs E., Buchholz A., Kleinmann B., Bredt W. Use of adherence protein of Mycoplasma pneumoniae as antigen for enzyme-linked immunosorbent assay (ELISA). Isr J Med Sci 1987; 23:709–712
    [Google Scholar]
  9. Hayflick L. Tissue cultures and mycoplasmas. Texas Reports on Biology and Medicine 1965; 23:285–303
    [Google Scholar]
  10. Van Kuppeveld F.J.M., Van der Logt J.T.M., Angulo A.F. Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification. Appl Environ Microbiol 1992; 58:2606–2615
    [Google Scholar]
  11. Fritsche M., Haessler C., Brandner G. Induction of nuclear accumulation of the tumor-suppressor protein p53 by DNA-damaging agents. Oncogene 1993; 8:307–318
    [Google Scholar]
  12. Jacobs E., Bennewitz A., Bredt W. Reaction pattern of human anti-Mycoplasma pneumoniae antibodies in enzyme-linked immunosorbent assays and immunoblotting. J Clin Microbiol 1986; 23:517–522
    [Google Scholar]
  13. Jacobs E., Gerstenecker B., Mader B. Binding sites of attachment-inhibiting monoclonal antibodies and of patients on peptide fragments of the Mycoplasma pneumoniae adhesin. Infect Immun 1988; 57:685–688
    [Google Scholar]
  14. Jacobs E., Clad A. Electroelution of fixed and stained membrane proteins from preparative sodium dodecyl sulfate-polyacrylamide gels into a membrane trap. Anal Biochem 1986; 154:583–589
    [Google Scholar]
  15. Kleemola M., Raty R., Karjalainen J., Schuy W., Gerstenecker B., Jacobs E. Evaluation of an antigen-capture enzyme immunoassay for rapid diagnosis of Mycoplasma pneumoniae infection. Eur J Clin Microbiol Infect Dis 1993; 12:872–875
    [Google Scholar]
  16. Bemet C., Garret M., de Barbeyrac B., Bebear C., Bonnet J. Detection of Mycoplasma pneumoniae by using the polymerase chain reaction. J Clin Microbiol 1989; 27:2492–2496
    [Google Scholar]
  17. Jacobs E. Serological diagnosis of Mycoplasma pneumonia infections: a critical review of current procedures. Clin Infect Dis 1993; 17: Suppl 1S79–82
    [Google Scholar]
  18. Minaschek G., Groschel-Stewart U., Blum S., Bereiter-Hahn J. Microcompartmentation of glycolytic enzymes in cultured cells. Eur J Cell Biol 1992; 58:418–428
    [Google Scholar]
  19. Pietsch K., Ehlers S., Jacobs E. Cytokine gene expression in the lungs of BALB/c mice during primary and secondary intranasal infections with Mycoplasma pneumoniae. Microbiology 1994; 140:2043–2048
    [Google Scholar]
  20. Jacobs E., Stuhlert A., Drews M. Host reactions to Mycoplasma pneumoniae infections in guinea-pigs preimmunized systematically with the adhesin of this pathogen. Microb Pathog 1988; 5:259–265
    [Google Scholar]
  21. Lind K., Hoier-Madsen M., Wiik A., Clyde W.A. Antibodies to the mitotic spindle apparatus in patients with Mycoplasma pneumoniae infection. Immunol Infect Dis 1992; 2:249–255
    [Google Scholar]
  22. Moodie F.D., Leaker B., Cambridge G., Totty N.F., Segal A.W. Alpha-enolase: a novel cytosolic autoantigen in ANCA positive vasculitis. Kidney Int 1993; 43:675–681
    [Google Scholar]
  23. Clark H.W. The potential role of mycoplasmas as autoantigensand immune complexes in chronic vascular pathogenesis. Am J Primatol 1991; 24:235–243
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-43-6-422
Loading
/content/journal/jmm/10.1099/00222615-43-6-422
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error