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Volume 43, Issue 5

An 51 -like integrin receptor mediates the binding of less pathogenic species to fibronectin No Access

Abstract

Summary

The present study was undertaken to investigate whether less pathogenic species () express a fibronectin receptor (FNr) antigenically related to α5βl integrin, which mediates their binding to fibronectin (FN). By flow cytometric analysis, a monoclonal antibody (MAb) directed against human α5 integrin subunit (clone SAM-1) and two different antisera to FNr positively stained , with the greatest expression observed for No or only marginal immunoreactivity was found on yeasts specifically adhered to FN; higher levels of adhesion were found for with respect to Less pathogenic Candida spp. bound to the Arg-Gly-Asp (RGD) containing 120-kDa fragment of FN and adhesion to intact FN was markedly inhibited by Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP), but not by Gly-Arg-Gly-Glu-Ser-Pro (GRGESP) peptides. In addition, anti-α5 SAM-1 MAb and both anti-FNr antisera strongly blocked binding of less pathogenic spp. to FN. Overall, these results indicate that less pathogenic spp., including , express a receptor antigenically related to α5βl integrin which mediates their adhesion to FN.

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© J. MED. MICROBIOL. 1995
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1995-11-01
2025-03-17
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References

  1. Gold J. W. M. Opportunistic fungal infections in patients with neoplastic disease. Am J Med 1984; 76:458–63
     [Google Scholar]
  2. Odds F. C. (ed) Disseminated candidosis (Candida septicemia). In Candida and candidosis 2nd edn London: Bailliere Tindall; 1988206
     [Google Scholar]
  3. Komshian S. V., Uwaydah A. K., Sobel J. D., Crane L. R. Fungemia caused by Candida species and Torulopsis glabrata in the hospitalized patients: frequency, characteristics, and evaluation of factors influencing outcome. Rev Infect Dis 1989; 11:379–390
     [Google Scholar]
  4. Meunier F. Candidiasis. Ear J Clin Microbiol Infect Dis 1989; 8:438–447
     [Google Scholar]
  5. Banerjee S. N., Emori T. G., Culver D. H. Secular trends in nosocomial primary bloodstream infections in the United States. 1980 1989. National Nosocomial Infections Surveillance System. Am J Med 1991; 91: Suppl 3B86S–89S
     [Google Scholar]
  6. Klein R. S., Harris C. A., Small C. B., Moll B., Lesser M., Friedland G. H. Oral candidasis in high-risk patients as the initial manifestation of the acquired immunodeficiency syndrome. N Engl J Med 1984; 311:354–358
     [Google Scholar]
  7. Armstrong D. Opportunistic infections in the acquired immune deficiency syndrome. Semin Oncol 1987; 142: Suppl 340–47
     [Google Scholar]
  8. Stellbrink H. J., Albrech H., Fenske S., Koperski K. Candida krusei sepsis in HIV infection. AIDS 1992; 6:746–748
     [Google Scholar]
  9. Bach M. C., Sahyoun A., Adler J. L. High incidence of fungus infections in renal transplantation patients treated with antilymphocyte and conventional immunosuppression. Transpl Proc 1973; 5:549–553
     [Google Scholar]
  10. Goodrich J. M., Reed E. C., Mori M. Clinical features and analysis of risk factors for invasive candidal infection after marrow transplantation. J Infect Dis 1991; 164:731–740
     [Google Scholar]
  11. Wingard J. R., Merz W. G., Rinaldi M. G., Johnson T. R., Karp J. E., Saral R. Increase in Candida krusei infection among patients with bone marrow transplantation and neutropenia treated prophylactically with fluconazole. N Engl J Med 1991; 325:1274–1277
     [Google Scholar]
  12. Bodey G. P. Candidiasis in cancer patients. Am J Med 1984; 77:(4D)13–19
     [Google Scholar]
  13. Maksymuik A. W., Thongprasert S., Hopfer R., Luna M., Fainstein V., Bodey G. P. Systematic candidiasis in cancer patients. Am J Med 1984; 77:(4D)20–27
     [Google Scholar]
  14. Whimbey E., Kiehn T. E., Brannon P., Blevins A., Armstrong D. Bacteremia and fungemia in patients with neoplastic disease. Am J Med 1987; 82:723–730
     [Google Scholar]
  15. Sandin R. L., Meier C. S., Crowder M. L., Greene J. N. Concurrent isolation of Candida krusei and Candida tropicalis from multiple blood cultures in a patient with acute leukemia. Arch Pathol Lab Med 1993; 117:521–523
     [Google Scholar]
  16. Hostetter M. K. Adhesins and ligands involved in the interaction of Candida spp. with epithelial and endothelial surfaces. Clin Microbiol Rev 1994; 7:29–42
     [Google Scholar]
  17. Dick J. D., Merz W. G., Saral R. Incidence of polyene-resistant yeasts recovered from clinical specimens. Antimicrob Agents Chemother 1980; 18:158–163
     [Google Scholar]
  18. Drutz D. J., Lehrer R. I. Development of amphotericin B-resistant Candida tropicalis in a patient with defective leukocyte function. Am J Med Sci 1978; 276:77–92
     [Google Scholar]
  19. Nobre G., Mendes E., Charrua M. J., Cruz O. Ketoconazole resistance in Torulopsis glabrata. My copathologia 1989; 107:51–55
     [Google Scholar]
  20. Rubinstein E., Noriega E. R., Simberkoff M. S., Holzmann R., Rahal J. J. Fungal endocarditis: analysis of 24 cases and review of the literature. Medicine 1975; 54:331–334
     [Google Scholar]
  21. Nolte S. Clinical quiz. Obstructive fungal pyelonephritis. Pediatr Nephrol 1992; 6:119–120
     [Google Scholar]
  22. Edwards J. E., Montgomerie J. Z., Ishida K., Morrison J. O., Guze L. B. Experimental hematogenous endophthalmitis due to Candida: species variation in ocular pathogenicity. J Infect Dis 1977; 135:294–297
     [Google Scholar]
  23. McQuillen D. P., Zingman B. S., Meunier F., Levitz S. M. Invasive infections due to Candida krusei: report of ten cases of fungemia that include three cases of endophthalmitis. Clin Infect Dis 1992; 14:472–478
     [Google Scholar]
  24. Imahori S. C., Papademetriou T., Ogliela D. M. Torulopsis glabrata osteomyelitis. A case report. Clin Orthop 1987; 219:214–220
     [Google Scholar]
  25. Delaporte E., Piette F., Ehrsam E., Flipo R. M., Fruit J., Bergoend H. Chronic cutaneous candidiasis caused by Candida krusei. Ann Dermatol Venereol 1992; 119:863–865
     [Google Scholar]
  26. Klotz S. A. Fungal adherence to the vascular compartment: a critical step in the pathogenesis of disseminated candidiasis. Clin Infect Dis 1992; 14:340–347
     [Google Scholar]
  27. Douglas L. J. Adhesion of Candida species to epithelial surfaces. Crit Rev Microbiol 1987; 15:27–43
     [Google Scholar]
  28. Calderone R. A., Braun P. C. Adherence and receptor relationships of Candida albicans. Microbiol Rev 1991; 55:1–20
     [Google Scholar]
  29. Tronchin G., Bouchara J. P., Annaix V., Robert R., Senet J. M. Fungal cell adhesion molecules in Candida albicans. Eur J Epidemiol 1991; 7:23–33
     [Google Scholar]
  30. Skerl K. G., Calderone R. A., Segal E., Sreevalson T., Scheld W. M. In vitro binding of Candida albicans yeast cells to human fibronectin. Can J Microbiol 1984; 30:221–227
     [Google Scholar]
  31. Klotz S. A. The adherence of Candida yeasts to human and bovine vascular endothelium and subendothelial extracellular matrix. FEMS Microbiol Lett 1987; 48:201–205
     [Google Scholar]
  32. Bouchara J.-P., Tronchin G., Annaix V., Robert R., Senet J.-M. Laminin receptors on Candida albicans germ tubes. Infect Immun 1990; 58:48–54
     [Google Scholar]
  33. López-Ribot J. L., Casanova M., Monteagudo C., Sepulveda P., Martinez J. P. Evidence for the presence of a high-affinity laminin receptor-like molecule on the surface of Candida albicans yeast cells. Infect Immun 1994; 62:742–746
     [Google Scholar]
  34. Klotz S. A., Rutten M. J., Smith R. L., Babcock S. R., Cunningham M. D. Adherence of Candida albicans to immobilized extracellular matrix proteins is mediated by calcium-dependent surface glycoproteins. Microb Pat hog 1993; 14:133–147
     [Google Scholar]
  35. Jakab E., Paulsson M., Ascencio F., Ljungh A. Expression of vitronectin and fibronectin binding by Candida albicans yeast cells. APMIS 1993; 101:187–193
     [Google Scholar]
  36. Scheld W. M., Strunk R. W., Balian G., Calderone R. A. Microbial adhesion to fibronectin in vivo correlates with production of endocarditis in rabbits. Proc Soc Exp Biol Med 1985; 180:474–482
     [Google Scholar]
  37. Segal E. Pathogenesis in human mycoses: role of adhesion to host surfaces. Microbiol Sci 1987; 4:344–347
     [Google Scholar]
  38. Calderone R. A., Scheld W. M. Role of fibronectin in the patho genesis of candidal infections. Rev Infect Dis 1987; 9: Suppl 4S400–S403
     [Google Scholar]
  39. Ruoslahti E. Integrins. J Clin Invest 1991; 87:1–5
     [Google Scholar]
  40. Hynes R. O. Integrins: versatility, modulation, and signalling in cell adhesion. Cell 1992; 69:11–25
     [Google Scholar]
  41. Takada Y., Huang C., Hemler M. E. Fibronectin receptor structures in the VLA family of heterodimers. Nature 1987; 326:607–609
     [Google Scholar]
  42. Klotz S. A., Smith R. L. A fibronectin receptor on Candida albicans mediates adherence of the fungus to extracellular matrix. J Infect Dis 1991; 163:604–610
     [Google Scholar]
  43. Santoni G., Gismondi A., Jin Hong L. Candida albicans expresses a fibronectin receptor antigenically related to α5β1 integrin. Microbiol 1994; 140:2971–2979
     [Google Scholar]
  44. Conforti G., Zanetti A., Colella S. Interaction of fibronectin with cultured human endothelial cells: characterization of the specific receptor. Blood 1989; 73:1576–1585
     [Google Scholar]
  45. Torosantucci A., Boccanera M., Casalinuovo I., Pellegrini G., Cassone A. Differences in the antigenic expression of immunomodulatory mannoprotein constituents on yeast and mycelial forms of Candida albicans. J Gen Microbiol 1990; 136:1421–1428
     [Google Scholar]
  46. Djeu J. Y., Parapanissios A., Halkias D., Friedman H. A rapid [3H]-glucose incorporation assay for determination of lymphoid cell-mediated inhibition of Candida albicans growth. J Immunol Methods 1986; 92:73–77
     [Google Scholar]
  47. Pierschbacher M. D., Hayman E. G., Ruoslahti E. Location of cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule. Cell 1981; 26:259–267
     [Google Scholar]
  48. Kalo A., Segal E., Sahar E., Dayan D. Interaction of Candida albicans with mucosal surfaces: involvement of fibronectin in adherence. J Infect Dis 1988; 157:1253–1256
     [Google Scholar]
  49. Bendel C. M., Hostetter M. K. Distinct mechanisms of epithelial adhesion for Candida albicans and Candida tropicalis. Identification of the participating ligands and development of inhibitory peptides. J Clin Invest 1993; 92:1840–1849
     [Google Scholar]
  50. Ollert M. K., Söhnchen R., Korting H. C., Ollert U., Braütigam S., Brautigam W. Mechanisms of adherence of Candida albicans to cultured human epidermal keratinocytes. Infect Immun 1993; 61:4560–4568
     [Google Scholar]
  51. Gustafson K. S., Vercellotti G. M., Bendel C. M., Hostetter M. K. Molecular mimicry in Candida albicans. Role of an integrin analogue in adhesion of the yeast to human endothelium. J Clin Invest 1991; 87:1896–1902
     [Google Scholar]
  52. King R. D., Lee J. C., Morris A. L. Adherence of Candida albicans and other Candida species to mucosal epithelial cells. Infect Immun 1980; 27:667–674
     [Google Scholar]
  53. Ray T. L., Digre K. B., Payne C. D. Adherence of Candida species to human epidermal comeocytes and buccal mucosal cells: correlation with cutaneous pathogenicity. J Invest Dermatol 1984; 83:37–41
     [Google Scholar]
  54. Klotz S. A., Drutz D. J., Harrison J. L., Huppert H. Adherence and penetration of vascular endothelium by Candida yeasts. Infect Immun 1983; 42:374–384
     [Google Scholar]
  55. Rotrosen D., Edwards J. E., Gibson T. R., Moore J. C., Cohen A. H., Green I. Adherence of Candida to cultured vascular endothelial cells: mechanisms of attachment and endothelial cell penetration. J Infect Dis 1985; 152:1264–1274
     [Google Scholar]
  56. Klotz S. A., Smith R. L., Stewart B. W. Effect of an arginine-glycine-aspartic acid-containing peptide on hematogenous candidal infections in rabbits. Antimicrob Agents Chemother 1992; 36:132–136
     [Google Scholar]
  57. Lehrer N., Segal E., Cihlar R., Calderone R. A. Pathogenesis of vaginal candidiasis: studies with a mutant which has reduced ability to adhere in vitro. J Med Vet Mycol 1986; 24:127–132
     [Google Scholar]
  58. Heidenreich F., Dierich M. P. Candida albicans and Candida stellatoidea, in contrast to other Candida species, bind iC3b and C3d but not C3b. Infect Immun 1985; 50:598–600
     [Google Scholar]
  59. Edwards J. E., Gaither T. A., O’Shea J. J. Expression of specific binding sites on Candida with functional and antigenic characteristics of human complement receptors. J Immunol 1986; 137:3577–3583
     [Google Scholar]
  60. Hostetter M. K., Lorenz J. S., Preus L., Kendrick K. E. The iC3b receptor on Candida albicans: Subcellular localization and modulation of receptor expression by glucose. J Infect Dis 1990; 161:761–768
     [Google Scholar]
/content/journal/jmm/10.1099/00222615-43-5-360
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An α5β1 -like integrin receptor mediates the binding of less pathogenic Candida species to fibronectin
J. Med. Microbiol. 43, 360 (1995); https://doi.org/10.1099/00222615-43-5-360
/content/journal/jmm/10.1099/00222615-43-5-360
/content/journal/jmm/10.1099/00222615-43-5-360
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