Prevalence of with heat-stable enterotoxin (NAG-ST) and cholera toxin genes; restriction fragment length polymorphisms of NAG-ST genes among O serogroups from a major shrimp production area in Thailand Free

Abstract

Summary

A total of 148 isolates from a major shrimp production area in Southern Thailand were examined by colony hybridisation for genes encoding heat-stable enterotoxin (NAG-ST) and cholera toxin (CT). Only non-O1 strains were found to harbour NAG-ST (14 of 146) whereas no strains hybridised with the CT probe. NAG-ST-positive non-O1 strains were isolated from shrimp farms situated close to urban areas. Five different O serogroups were found among NAG-ST positive non-O1 strains. Southern blot and restriction endonuclease analysis of NAG-ST-positive strains revealed a high degree of genetic divergence. A total of seven classes of enterotoxin gene patterns were found with dIII and RI restriction endonucleases. Enterotoxin gene patterns correlated with O-antigen expression in 84% of isolates tested. In combination with other molecular techniques Southern blot analysis with an NAG-ST oligonucleotide probe could be useful for studying the molecular epidemiology of non-O1 strains.

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1995-09-01
2024-03-28
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References

  1. Kaper J., Lockman H., Colwell R. R., Joseph S. W. Ecology, serology, and enterotoxin production of Vibrio cholerae in Chesapeake bay. Appl Environ Microbiol 1979; 37:91–103
    [Google Scholar]
  2. Kaysner C. A., Abeyta C., Wekell M. M., Depaola A., Stott R. F., Leitch J. M. Incidence of Vibrio cholerae from estuaries of the United States West Coast. Appl Environ Microbiol 1987; 53:1344–1348
    [Google Scholar]
  3. Adkins H. J., Escamilla J., Santiago L. T., Ranoa C., Echeverria P., Cross J. H. Two-year survey of etiologic agents of diarrheal disease at San Lazaro hospital, Manila, Republic of the Philippines. J Clin Microbiol 1987; 25:1143–1147
    [Google Scholar]
  4. Desenclos J. C., Klontz K. C., Wolfe L. E., Hoecheri S. The risk of Vibrio illness in the Florida raw oyster eating population, 1981-1988. Am J Epidemiol 1991; 134:290–297
    [Google Scholar]
  5. Piersimoni C., Morbiducci V., Scalise G. Non-0l Vibrio cholera gastroenteritis and bacteraemia. Lancet 1991; 337:791–792
    [Google Scholar]
  6. Csavas I. The impact of aquaculture on the shrimp industry. Infofish International 1993; 1:42–48
    [Google Scholar]
  7. Reilly P. J. A., Twiddy D. R. Salmonella and Vibrio cholerae in brackish water cultured tropical prawns. Int J Food Microbiol 1992; 16:293–301
    [Google Scholar]
  8. Nair G. B., Bhadra R. K., Ramamurthy T., Ramesh A. Pal SCVibrio cholerae and other vibrios associated with paddy field cultured prawns. Food Microbiol 1991; 8:203–208
    [Google Scholar]
  9. Nair G. B., Oku Y., Takeda Y. Toxin profiles of Vibrio cholerae non-0l from environmental sources in Calcutta, India. Appl Environ Microbiol 1988; 54:3180–3182
    [Google Scholar]
  10. Yamamoto K., Takeda Y., Miwatani T., Craig J. P. Purification and some properties of a non-0l Vibrio cholerae entero-toxin that is identical to cholera enterotoxin. Infect Immun 1983; 39:1128–1135
    [Google Scholar]
  11. Honda T., Arita M., Takeda T., Yoh M., Miwatani T. Non-0l Vibrio cholerae produces two newly identified toxins related to Vibrio parahaemolyticus haemolysin and Escherichia coli heat-stable enterotoxin. Lancet 1985; 2:163–164
    [Google Scholar]
  12. Arita M., Takeda T., Honda T., Miwatani T. Purification and characterization of Vibrio cholerae non-Ol heat-stable enterotoxin. Infect Immun 1986; 52:45–49
    [Google Scholar]
  13. Craig J. P., Yamamoto K., Takeda Y., Miwatani T. Production of cholera-like enterotoxin by a Vibrio cholerae non-01 strain isolated from the environment. Infect Immun 1981; 34:90–97
    [Google Scholar]
  14. Dalsgaard A., Huss H. H., H-Kittikun A., Larsen J. L. Prevalence of Vibrio cholerae in a major shrimp production area in Thailand. Inf J Food Microbiol 1995 in press
    [Google Scholar]
  15. Hoge C. W., Sethabutr O., Bodhidatta L., Echeverria P., Robertson D. C., Morris J. G. Use of a synthetic oligo-nucleotide probe to detect strains of non-serovar 01 Vibrio cholerae carrying the gene for heat-stable enterotoxin (NAG-ST). J Clin Microbiol 1990; 28:1473–1476
    [Google Scholar]
  16. Ogawa A., Kato J. I., Watanabe H., Nair B. G., Takeda T. Cloning and nucleotide sequence of a heat-stable enterotoxin gene from Vibrio cholerae non-Ol isolated from a patient with traveler’s diarrhea. Infect Immun 1990; 58:3325–3329
    [Google Scholar]
  17. Arita M., Honda T., Miwatani T., Ohmori K., Takao T., Shimonishi Y. Purification and characterization of a new heat-stable enterotoxin produced by Vibrio cholerae non-0l serogroup Hakata. Infect Immun 1991; 59:2186–2188
    [Google Scholar]
  18. Wright A. C., Guo Y., Johnson J. A., Nataro J. P., Morris J. G. Development and testing of a nonradioactive DNA oligo-nucleotide probe that is specific for Vibrio cholerae cholera toxin. J Clin Microbiol 1992; 30:2302–2306
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T. (eds) Molecular cloning: a laboratory manual. 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; 1989; 1131
    [Google Scholar]
  20. Bagchi K., Echeverria P., Arthur J. D., Sethabutr O., Serichantalergs O., Hoge C. W. Epidemic of diarrhea caused by Vibrio cholerae non-Ol that produced heat-stable toxin among Khmers in a camp in Thailand. J Clin Microbiol 1993; 31:1315–1317
    [Google Scholar]
  21. Maas R. An improved colony hybridization method with a significantly increased sensitivity for detection of single genes. Plasmid 1983; 10:296–298
    [Google Scholar]
  22. Oprandy J. J., Thornton S. A., Gardiner C. H., Burr L. D., Batchelor R., Bourgeois A. L. Alkaline phosphatase-conjugated oligo-nucleotide probes for enterotoxigenic Escherichia coli in travelers to South America and West Africa. J Clin Microbiol 1988; 26:92–95
    [Google Scholar]
  23. Morris J. G., Takeda T., Tall B. D. Experimental non-01 group 1 Vibrio cholerae gastroenteritis in humans. J Clin Invest 1990; 85:697–705
    [Google Scholar]
  24. Hanchalay S., Seriwatana J., Echeverria P. Non-0l Vibrio cholerae in Thailand: homology with cloned cholera toxin genes. J Clin Microbiol 1985; 21:288–289
    [Google Scholar]
  25. Shimada T., Arakawa E., Itoh K. Extended serotyping scheme for Vibrio cholerae. Curr Microbiol 1994; 28:175–178
    [Google Scholar]
  26. Murray M. G. Thompson WF. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 1980; 8:4321–4325
    [Google Scholar]
  27. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 1975; 98:503–517
    [Google Scholar]
  28. Tauxe R., Blake P., Olsvik G., Wachsmuth I. K. The future of cholera: persistence, change, and an expanding research agenda. In Wachsmuth I. K., Blake P. A., Olsvik O. (eds) Vibrio cholerae and cholera: molecular to global perspectives Washington, DC: ASM Press; 1994443–453
    [Google Scholar]
  29. Pal A., Ramamurthy T., Bhadra R. K. Reassessment of the prevalence of heat-stable enterotoxin (NAG-ST) among environmental Vibrio cholerae non-0l strains isolated from Calcutta, India, by using a NAG-ST DNA probe. Appl Environ Microbiol 1992; 58:2485–2489
    [Google Scholar]
  30. Rabbani G. H., Mahalanabis D. New strains of Vibrio cholera 0139 in India and Bangladesh: lessons from the recent epidemics. J Diarrhoeal Dis Res 1993; 11:63–66
    [Google Scholar]
  31. Ramamurthy T., Garg S., Sharma R. Emergence of novel strain of Vibrio cholerae with epidemic potential in southern and eastern India. Lancet 1993; 341:703–704
    [Google Scholar]
  32. Kaper J. B., Nataro J. P., Roberts N. C., Siebeling R. J., Bradford H. B. Molecular epidemiology of non-0l Vibrio cholerae and Vibrio mimicus in the U.S. Gulf Coast region. J Clin Microbiol 1986; 23:652–654
    [Google Scholar]
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