1887

Abstract

Summary

Serotyping of based on the differential recognition by a panel of six monoclonal antibodies (MAbs) against Protein IB (PIB) is a valuable tool for studying the epidemiology of gonorrhoea. However, the predominance of certain serovars in specific geographic regions necessitates the development of new MAbs or new genotyping approaches. Nucleotide and amino-acid sequence analysis of PIB from strains within the IB-3 and IB-7 serovars revealed strain differences within the same serovar. Based on the generation of PIB nucleotide and deduced amino-acid sequences that centred on amino-acid residues 196 and 237, eight serovar IB-3 strains and nine serovar IB-7 strains were separately subdivided into five groups. Intra-serovar differences were also established by pulsed-field gel electrophoresis (PFGE) of macro-restriction fragments generated by SpeI- and NheI-cleavage of genomic DNA. There was good correlation between the results based on PIB gene PCR-sequencing and those based on PFGE analysis of macro-restriction fragment patterns. These data demonstrate the high precision of PFGE analysis and indicate that this approach can be used as a rapid epidemiological subtyping system for major serovars of .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-43-3-201
1995-09-01
2022-12-01
Loading full text...

Full text loading...

/deliver/fulltext/jmm/43/3/medmicro-43-3-201.html?itemId=/content/journal/jmm/10.1099/00222615-43-3-201&mimeType=html&fmt=ahah

References

  1. Kihlstrom E., Danielsson D. Advances in biology, management and prevention of infections caused by Chlamydia trachomatis and Neisseria gonorrhoeae. Curr Opin Infect Dis 1994; 7:25–33
    [Google Scholar]
  2. Kohl P. K., Ison C. A., Danielsson D., Knapp J. S., Petzoldt D. Current status of serotyping of Neisseria gonorrhoeae. Eur JE pidemiol 1990; 6:91–95
    [Google Scholar]
  3. Poh C. L., Ocampo J. C., Sng E. H., Bygdeman S. M. Characterisation of PPNG and non-PPNG Neisseria gonorrhoeae isolates from Singapore. Genitourin Med 1991; 67:389–393
    [Google Scholar]
  4. Sarafian S. K., Knapp J. S. Molecular epidemiology of gonorrhea. Clin Microbiol Rev 1989; 2: Suppl S49–S55
    [Google Scholar]
  5. Lau Q. C., Chow V. T. K., Poh C. L. Polymerase chain reaction and direct sequencing of Neisseria gonorrhoeae protein IB gene: partial nucleotide and amino acid sequence analysis of strains S4, SI 1, S48 (serovar IB 4) and S34 (serovar IB 5). Med Microbiol Immunol 1993; 182:137–145
    [Google Scholar]
  6. Poh C. L., Lau Q. C. Subtyping of Neisseria gonorrhoeae auxotype-serovar groups by pulsed-field gel electrophoresis. J Med Microbiol 1993; 38:366–370
    [Google Scholar]
  7. Butt N. J., Lambden P. R., Heckels J. E. The nucleotide sequence of the por gene from Neisseria gonorrhoeae strain P9 encoding outer membrane protein PIB. Nucleic Acids Res 1990; 14:4258
    [Google Scholar]
  8. Butt N. J., Virji M., Vayreda F., Lambden P. R., Heckels J. E. Gonococcal outer-membrane protein PIB: comparative sequence analysis and localization of epitopes which are recognized by type-specific and cross-reacting monoclonal antibodies. J Gen Microbiol 1990; 136:2165–2172
    [Google Scholar]
  9. Maslow J. N., Mulligan M. E., Arbeit R. D. Molecular epidemiology: application of contemporary techniques to the typing of microorganisms. Clin Infect Dis 1993; 17:153–164
    [Google Scholar]
  10. Prevost G., Jaulhac B., Piemont Y. DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates. J Clin Microbiol 1992; 30:967–973
    [Google Scholar]
  11. Johnson W. M., Bernard K., Marrie T. J., Tyler S. D. Discriminatory genomic fingerprinting of Legionella pneumophila by pulsed-field electrophoresis. J Clin Microbiol 1994; 32:2620–2621
    [Google Scholar]
  12. Chow V. T. K., Lau Q. C., Poh C. L. Mapping of serovar-specific monoclonal antibody epitopes by DNA and amino acid sequence analysis of Neisseria gonorrhoeae outer membrane protein IB strains. Immunol Infect Dis 1994; 4:202–206
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-43-3-201
Loading
/content/journal/jmm/10.1099/00222615-43-3-201
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error