1887

Abstract

Summary

Co-agglutination (coagg) and latex agglutination (LA) tests were used for the detection of serotype Typhi Vi and Barber protein (BP) antigens in sera from five groups of individuals (A–E). Group A consisted of 30 blood culture-positive cases of typhoid fever and group B consisted of 30 suspected cases of typhoid fever who had sterile blood cultures but positive Widal tests. Thirty cases of pyrexia of unknown origin (PUO) were placed in group C, while group D consisted of 15 cases of septicaemia caused by organisms other than serotype Typhi. Group E comprised 50 normal healthy individuals with no history of typhoid fever or TAB vaccination in the previous 5 years. The Vi-LA test performed best with 96.7% of group A sera and 90% of group B sera giving positive results. No false positive results and only 2.58% false negative results were obtained with this test. Considering patients with positive blood culture results or positive Widal tests as true positives, the sensitivities of the Vi-LA, BP-LA, Vi-coagg and BP-coagg tests were 93.3, 91.7, 83-3 and 86.7%, respectively. The specificities of these tests were 100, 98.5, 98.5 and 98.5%, respectively. It is suggested that the Vi-LA test can be used for the rapid and early diagnosis of typhoid fever.

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/content/journal/jmm/10.1099/00222615-43-3-185
1995-09-01
2024-04-24
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References

  1. Gilman R. H., Terminel M., Levine M. M., Hemandez-Mendoza P., Homick R. B. Relative efficacy of blood, urine, rectal swab, bone-marrow and rose-spot cultures for recovery of Salmonella typhi in typhoid fever. Lancet 1975; 1:1211–1213
    [Google Scholar]
  2. Levine M. M., Grados O., Gilman R. H., Woodward W. E., Solis-Plaza R., Waldman W. Diagnostic value of the Widal test in areas endemic for typhoid fever. Am J Trop Med Hyg 1978; 27:795–800
    [Google Scholar]
  3. Abraham G., Teklu B., Gedebu M., Selassie G. H., Azene G. Diagnostic value of the Widal test. Trop Geogr Med 1981; 33:329–333
    [Google Scholar]
  4. Appassakij H., Bunchuin N., Sarasombath S. Enzyme-linked immunosorbent assay for detection of Salmonella typhi protein antigen. J Clin Microbiol 1987; 25:273–277
    [Google Scholar]
  5. Gupta A. K., Rao K. M. Simultaneous detection of Salmonellatyphi antigen and antibody in serum by counter-immunoelectrophoresis for an early and rapid diagnosis of typhoid fever. J Immunol Methods 1979; 30:349–353
    [Google Scholar]
  6. John T. J., Sivadasan K., Kurien B. Evaluation of passive bacterial agglutination for the diagnosis of typhoid fever. J Clin Microbiol 1984; 20:751–753
    [Google Scholar]
  7. Rockhill R. C., Rumans L. W., Lesmana M., Dennis D. T. Detection of Salmonella typhi D, Vi, and d antigens by slide coagglutination, in urine from patients with typhoid fever. J Clin Microbiol 1980; 11:213–216
    [Google Scholar]
  8. Taylor D. N., Harris J. R., Barrett T. J. Detection of urinary Vi antigen as a diagnostic test for typhoid fever. J Clin Microbiol 1983; 18:872–876
    [Google Scholar]
  9. Barber C., Vladoianu I. R., Dimache G. Contributions to the study of Salmonella. Immunological specificity of proteins separated from Salmonella typhi. Immunology 1966; 11:287–296
    [Google Scholar]
  10. Hartree E. F. Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem 1972; 48:A22–A21
    [Google Scholar]
  11. Ekpo P., Sarasombath S., Banchuin N., Sirisinha S. Monoclonal antibodies to 52-Kilodalton protein of Salmonella typhi. J Clin Microbiol 1990; 28:1818–1821
    [Google Scholar]
  12. Williams C. A., Chase M. W. Methods in immunology and immunochemistry. vol II Physical and chemical methods New York: Academic Press;
    [Google Scholar]
  13. Mukherjee C., Malik A., Khan H. M., Malik A. Rapid diagnosis of typhoid fever by co-agglutination in an Indian hospital. J Med Microbiol 1993; 39:74–77
    [Google Scholar]
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