1887

Abstract

Summary

Amplification of the region separating the genes coding for the two rRNA species 16S and 23S was performed with 56 strains of several mycobacterial species, including 21 clinical isolates of and the type strain ATCC 12478. On the basis of PCR product profiles, the previously suggested heterogeneity of species was confirmed. Three subgroups were identified; members of the first subgroup showed the same PCR profile as the reference strain. Different profiles were obtained for the two other subgroups. Amplification of the 16S–23S spacer is rapid and simple and, consequently, may be a helpful tool for identification and characterisation of isolates in epidemiological analysis.

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/content/journal/jmm/10.1099/00222615-43-2-156
1995-08-01
2024-11-06
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References

  1. Wolinsky E. Nontuberculous mycobacteria and associated diseases. Am Rev Respir Dis 1979; 119:107–159
    [Google Scholar]
  2. Selik R. M., Starcher E. T., Curran J. W. Opportunistic diseases reported in AIDS patients: frequencies, associations, and trends. AIDS 1987; 1:175–182
    [Google Scholar]
  3. Sherer R., Sable R. Sonnenberg S. Disseminated infection with Mycobacterium kansasii in the Acquired Immuno-deficiency Syndrome. Ann Intern Med 1986; 105:710–712
    [Google Scholar]
  4. Ross B. C., Jackson K., Yang M., Sievers A., Dwyer B. Identification of a genetically distinct subspecies of Mycobacterium kansasii. J Clin Microbiol 1992; 30:2930–2933
    [Google Scholar]
  5. Yang M., Ross B. C., Dwyer B. Identification of an insertion sequence-like element in a subspecies of Mycobacterium kansasii. J Clin Microbiol 1993; 31:2074–2079
    [Google Scholar]
  6. de Lambalerie X., Zandotti C., Vignoli C., Bollet C., de Micco P. A one-step microbial DNA extraction method using “Chelex 100” suitable for gene amplification. Res Microbiol 1992; 143:785–790
    [Google Scholar]
  7. Jensen M. A., Webster J. A., Strauss N. Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Appl Environ Microbiol 1993; 59:945–952
    [Google Scholar]
  8. Brosius J., Dull T. J., Sleeter D. D., Noller H. F. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol 1981; 148:107–127
    [Google Scholar]
  9. Loughney K., Lund E., Dahlberg J. E. tRNA genes are found between the 16S and 23S rRNA genes in Bacillus subtilis. Nucleic Acid Res 1982; 10:1607–1624
    [Google Scholar]
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