1887

Abstract

Surmmary

Polyacrylamide-agarose gel electrophoresis was used to study polymorphism of lactate dehydrogenase (LDH), nucleoside phosphorylase (NSP), phosphoglucose isomerase (PGI), hydroxybutyrate dehydrogenase (HBD), adenylate kinase (ADK) and esterases of 44 strains of , 25 group G streptococcal strains, 11 “” strains, seven strains, four strains, three strains and seven strains. Analysis of LDH, NSP, PGI, HBD and ADK provided valuable interspecies differentiation, by showing that four groups of strains corresponded to the four known DNA homology groups. Esterases showed greater intraspecies variation than the other enzymes. The combined analysis of the six enzymes indicated 31 zymotypes among , 14 in group G streptococci and 11 in “” strains. This was shown to be an effective technique for typing pyogenic streptococci.

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1995-06-01
2024-04-25
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References

  1. Lancefield R. C. A serological differentiation of human and other groups of hemolytic streptococci. J Exp Med 1933; 57:571–595
    [Google Scholar]
  2. Jones D. Composition and differentiation of the genus Strep tococcus . In Skinner F. A., Quesnel L. B. (eds) Streptococci for Applied Bacteriology Symposium series no. 7 London: Academic Press Inc; 19781–49
    [Google Scholar]
  3. Bridge P. D., Sneath P. H. A. Numerical taxonomy of Streptococcus. J Gen Microbiol 1983; 129:565–597
    [Google Scholar]
  4. Farrow J. A. E., Collins M. D. Taxonomic studies on streptococci of serological groups C, G and L and possibly related taxa. Syst Appl Microbiol 1984; 5:483–493
    [Google Scholar]
  5. Kilpper-Balz R., Schleifer K. H. Nucleic acid hybridization and cell wall composition studies of pyogenic streptococci. FEMS Microbiol Lett 1984; 24:355–364
    [Google Scholar]
  6. Schleifer K. H., Kilpper-Balz R. Molecular and chemotaxonomic approaches to the classification of streptococci, enterococci and lactococci: a review. Syst Appl Microbiol 1987; 10:1–19
    [Google Scholar]
  7. Musser J. M., Hauser A. R., Kim M. H., Schlievert P. M., Nelson K., Selander R. K. Streptococcus pyogenes causing toxic-shock like syndrome and other invasive diseases: clonal diversity and pyrogenic exotoxin expression. Proc Natl Acad Sci USA 1991; 88:2668–2672
    [Google Scholar]
  8. Schwartz B., Facklam R. R., Breiman R. F. Changing epidemiology of group A streptococcal infection in the USA. Lancet 1990; 336:1167–1171
    [Google Scholar]
  9. Swift H. F., Wilson A. T., Lancefield R. C. Typing group A hemolytic streptococci by M precipitin reactions in ca pillary pipettes. J Exp Med 1943; 78:127–133
    [Google Scholar]
  10. Efstratiou A. Outbreaks of human infection caused by pyogenic streptococci of Lancefield groups C and G. J Med Microbiol 1989; 29:207–219
    [Google Scholar]
  11. Bouvet A., Geslin P., Kriz-Kuzemenska P., Blanc V., Devine C., Grimont F. Restricted association between biotypes and serotypes within group A streptococci. J Clin Microbiol 1994; 32:1312–1317
    [Google Scholar]
  12. Bingen E., Denamur E., Lambert-Zechovsky N. Mother-to-infant vertical transmission and cross-colonization of Streptococcus pyogenes confirmed by DNA restriction fragment length polymorphism analysis. J Infect Dis 1992; 165:147–150
    [Google Scholar]
  13. Cleary P. P., Kaplan F. L., Livdahl C., Skjold S. DNA fingerprints of Streptococcus pyogenes are M type specific. J Infect Dis 1988; 158:1317–1323
    [Google Scholar]
  14. Single L. A., Martin D. R. Clonal differences within M-types of the group A Streptococcus revealed by pulsed field gel electro phoresis. FEMS Microbiol Lett 1992; 91:85–90
    [Google Scholar]
  15. Seppala H., He Q., Osterblad M., Huovinen P. Typing of group A streptococci by random amplified polymorphic DNA analysis. J Clin Microbiol 1994; 32:1945–1948
    [Google Scholar]
  16. Gilmour M. N., Whittam T. S., Killian M., Selander R. K. Genetic relationships among the oral streptococci. J Bacteriol 1987; 169:5247–5257
    [Google Scholar]
  17. Goullet P. An esterase zymogram of Escherichia coli . J Gen Microbiol 1973; 77:27–35
    [Google Scholar]
  18. Goullet P., Picard B. Characterization of Enterobacter cloacae and E. sakazakii by electrophoretic polymorphism of acid phosphatase, esterases, and glutamate, lactate and malate dehydrogenases. J Gen Microbiol 1986; 132:3105–3112
    [Google Scholar]
  19. Goullet P., Picard B. Characterization of Yersinia enterocolitica, Y intermedia, Y aldovae, Y. frederiksenii, Y. kristensenii and Y. pseudotuberculosis by electrophoretic polymor phism of acid phosphatase, esterases and glutamate and malate dehydrogenases. J Gen Microbiol 1988; 134:317–325
    [Google Scholar]
  20. Goullet P., Picard B. The electrophoretic polymorphism of bacterial esterases. FEMS Microbiol Rev 1995; 16:7–31
    [Google Scholar]
  21. Picard B., Goullet P. Epidemiological complexity of hospital aeromonas infections revealed by electrophoretic typing of esterases. Epidemiol Infect 1987; 98:5–14
    [Google Scholar]
  22. Picard B., Goullet P. Epidemiological typing of Acinetobacter strains by esterase electrophoresis. FEMS Microbiol Lett 1990; 72:229–234
    [Google Scholar]
  23. Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electro phoresis for bacterial population genetics and systematics. Appl Environ Microbiol 1986; 51:873–884
    [Google Scholar]
  24. Selander R. K., Musser J. M., Caugant D. A., Gilmour M. N., Whittam T. S. Population genetics of pathogenic bacteria. Micro Pathog 1987; 3:1–7
    [Google Scholar]
  25. Musser J. M., Gray B. M., Schlievert P. M., Pichichero M. E. Strep tococcus pyogenes pharyngitis: characterization of strains by multilocus enzyme genotype, M and T protein serotype, and pyrogenic exotoxin gene probing. J Clin Microbiol 1992; 30:600–603
    [Google Scholar]
  26. Uriel J. Methode d’électrophorèse dans des gels d’acrylamide-agarose. Bull Soc Chim Biol 1966; 48:969–982
    [Google Scholar]
  27. Siciliano M., Shaw C. R. Separation and visualization of enzymes on gels. In Smith I. (ed) Chromatographic and electro phoretic techniques 4th edn Chicago: Yearbook Medical Publishers; 1976185–209
    [Google Scholar]
  28. Uriel J. Caracterisation des cholinesterases et d’autres esterases carboxyliques après électrophorèse et immuno-electrophorese en gélose (application a l’étude des esterases du serum humain normal). Ann Inst Pasteur 1961; 101:104–119
    [Google Scholar]
  29. Lebart L., Morineau A., Warwick K. M. Multivariate descriptive analysis: correspondence analysis and related techniques for large matrices. New York: Wiley-Interscience; 1984231
    [Google Scholar]
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