Experimental Legionnaires’ disease in SCID-Beige mice reconstituted with human leucocytes Free

Abstract

Surmmary

A new small animal model of experimental Legionnaires′ disease is described in which the reconstitution of SCID-Beige mice with human peripheral blood leucocytes permits the in-vivo growth of in the lungs of aerosol-challenged mice. Following infection, viable bacterial counts within the lungs of mice increased from 10 cfu/lung at the time of inoculation to a maximum of 10 cfu/lung by 48 h post-inoculation. Two types of disease were detected in the lungs of infected SCID-Beige mice. An acute exudative bronchiolitis and bronchopneumonia were seen in the most severely affected mice and, in the less severely affected mice, lesions of subacute or chronic disease were seen with thickening of alveolar walls and consolidation of lung tissue. Human cells did not appear to be involved directly in the pathology but were required for the establishment of infection. Immunohistological staining of lung tissue revealed substantial amounts of bacterial antigen distributed in a pattern similar to that seen in human Legionnaires’ disease.

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1995-06-01
2024-03-28
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References

  1. Fraser D. W., Tsai T. R., Orenstein W. Legionnaires’ disease. Description of an epidemic of pneumonia. N Engl J Med 1977; 297:1189–1197
    [Google Scholar]
  2. Baskerville A., Fitzgeorge R. B., Broster M., Hambleton P. Histopathology of experimental Legionnaires’ disease in guinea pigs, rhesus monkeys and marmosets. J Pathol 1983; 139:349–362
    [Google Scholar]
  3. Baskerville A., Fitzgeorge R. B., Broster M., Hambleton P., Dennis P. J. Experimental transmission of Legionnaires’ disease by exposure to aerosols of Legionella pneumophila . Lancet 1981; 2:1389–1390
    [Google Scholar]
  4. Blackmon J. A., Chandler F. W., Cherry W. B. Legionellosis. Am J Pathol 1981; 103:429–165
    [Google Scholar]
  5. Yoshida S.-I., Goto Y., Mizuguchi Y., Nomoto K., Skamene E. Genetic control of natural resistance in mouse macrophages regulating intracellular Legionella pneumophila multiplication in vitro. Infect Immun 1991; 59:428–432
    [Google Scholar]
  6. Yoshida S., Mizuguchi Y. Multiplication of Legionella pneumophila Philadelphia-1 in cultured peritoneal macrophages and its correlation to susceptibility of animals. Can J Microbiol 1986; 32:438–442
    [Google Scholar]
  7. Yamamoto Y., Klein T. W., Newton C. A., Widen R., Friedman H. Growth of Legionella pneumophila in thioglycolate-elicited peritoneal macrophages from A/J mice. Infect Immun 1988; 56:370–375
    [Google Scholar]
  8. Schaible U. E., Gay S., Museteanu C. Lyme borreliosis in the severe combined immunodeficiency (scid) mouse manifests predominantly in the joints, heart, and liver. Am J Pathol 1990; 137:811–820
    [Google Scholar]
  9. Mead J. R., Arrowood M. J., Sidwell R. W., Healey M. C. Chronic Cryptosporidium parvum infections in congenitally immunodeficient SCID and nude mice. J Infect Dis 1991; 163:1297–1304
    [Google Scholar]
  10. Mosier D. E., Gulizia R. J., Baird S. M., Wilson D. B. Transfer of a functional human immune system to mice with severe combined immunodeficiency. Nature 1988; 335:256–259
    [Google Scholar]
  11. Torbett B. E., Picchio G., Mosier D. E. Hu-PBL-SCID mice: a model for human immune function, AIDS, and lymphomagenesis. Immunol Rev 1991; 124:139–164
    [Google Scholar]
  12. Jepras R. I., Fitzgeorge R. B., Baskerville A. A comparison of virulence of two strains of Legionella pneumophila based on experimental aerosol infection of guinea-pigs. J Hyg 1985; 95:29–38
    [Google Scholar]
  13. Edelstein P. H. Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. J Clin Microbiol 1981; 14:298–303
    [Google Scholar]
  14. Fujio H., Yoshida S., Miyamoto H., Mitsuyama M., Mizuguchi Y. Investigation of the role of macrophages and endogenous interferon-gamma in natural resistance of mice against Legionella pneumophila infection. FEMS Microbiol Immunol 1992; 4:183–191
    [Google Scholar]
  15. Yamamoto Y., Klein T. W., Newton C., Friedman H. Differing macrophage and lymphocyte roles in resistance to Legionella pneumophila infection. J Immunol 1992; 148:584–589
    [Google Scholar]
  16. Benfield M. R., Witson J. C., Alter B. J., Bach F. H. Murine APC activation in the xenogenic MLC. Scand J Immunol 1993; 38:130–136
    [Google Scholar]
  17. Hoffmann-Fezer G., Kranz B., Gall C., Thierfelder S. Peritoneal sanctuary for human lymphopoiesis in SCID mice injected with human peripheral blood lymphocytes from Epstein-Barr virus-negative donors. Eur J Immunol 1992; 22:3161–3166
    [Google Scholar]
  18. Rechnitzer C., Diamant M., Pedersen B. K. Inhibition of human natural killer cell activity by Legionella pneumophila protease. Eur J Clin Microbiol Infect Dis 1989; 8:989–992
    [Google Scholar]
  19. Rechnitzer C., Kharazmi A. Effect of Legionella pneumophila cytotoxic protease on human neutrophil and monocyte function. Microb Pathog 1992; 12:115–125
    [Google Scholar]
  20. Sahney N. N., Lambe B. C., Summersgill J. T., Miller R. D. Inhibition of polymorphonuclear leukocyte function by Legionella pneumophila exoproducts. Microb Pathog 1990; 9:117–125
    [Google Scholar]
  21. Conlan J. W., Williams A., Ashworth L. A. E. Inactivation of human α l-antitrypsin by a tissue destructive protease of Legionella pneumophila . J Gen Microbiol 1988; 134:481–487
    [Google Scholar]
  22. Mintz C. S., Miller R. D., Gutgsell N. S., Malek T. Legionella pneumophila protease inactivates interleukin-2 and cleaves CD4 on human T cells. Infect Immun 1993; 61:3416–3421
    [Google Scholar]
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