1887

Abstract

Surmmary

Back mutations from the TRC-1 β-lactamase to the TEM-1 enzyme were selected . The revertant β-lactamase was obtained from strain J62.2 carrying plasmid pUK901 which encodes the TRC-1 β-lactamase. The revertant was obtained after repeated subculture of J62.2 (pUK901) in amoxycillin 512 mg/L for 5 days. The revertant β-lactamase had the same pI as TEM-1 (5.4) and had restored inhibition by clavulanic acid (ID50 reduced from 4.2 μm to 0.15 μm). The prevalence of these β-lactamases in the clinical population may be the result of a two-way flux, with mutations in both forward and backward directions.

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/content/journal/jmm/10.1099/00222615-42-6-429
1995-06-01
2019-11-12
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