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Volume 42, Issue 6

Human immunodeficiency virus type-1 can be detected in monocytes by polymerase chain reaction Free

Abstract

Surmmary

Lymphocytes and monocytes from 25 patients infected with human immunodeficiency virus type-1 (HIV-1)—13 asymptomatic, seven with the AIDS-related complex (ARC) and five with the acquired immunodeficiency syndrome (AIDS)—were lysed and subjected to PCR with three primer pairs: SK38/SK39 (), SK68/SK69 () and SK29/SK30 (). Amplified DNA was solution-hybridised with P-labelled probes (SK19, SK70 and SK31, respectively) and detected by PAGE-autoradiography. HIV-1 DNA was detected as follows. Asymptomatic patients: Monocytes— 61.5%, 100%, LTR 0%; lymphocytes— 100%, 92.3%, 53.84%. ARC patients: Monocytes— 71.4%, 57.1%, 0%; lymphocytes— 100%, 71.4%, 71.4%. AIDS patients: Monocytes— 80.0%, 100%, 0%; lymphocytes— 100%, 60%, 60%. The presence of HIV-1 DNA was confirmed in the monocyte fraction. In this cell subset, the gene-directed primers were the most effective for amplification, whereas the gene-directed primers failed to amplify HIV-1 DNA. The different pattern of amplification found in monocytes may suggest that these cells could be infected by a genetic variant of the virus.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© 1995 Pathological Society of Great Britain and Ireland
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1995-06-01
2024-04-20
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Human immunodeficiency virus type-1 can be detected in monocytes by polymerase chain reaction
J. Med. Microbiol. 42, 411 (1995); https://doi.org/10.1099/00222615-42-6-411
/content/journal/jmm/10.1099/00222615-42-6-411
/content/journal/jmm/10.1099/00222615-42-6-411
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