Lymphocytes and monocytes from 25 patients infected with human immunodeficiency virus type-1 (HIV-1)–13 asymptomatic, seven with the AIDS-related complex (ARC) and five with the acquired immunodeficiency syndrome (AIDS)–were lysed and subjected to PCR with three primer pairs: SK38/SK39 (), SK68/SK69 () and SK29/SK30 (). Amplified DNA was solution-hybridised with P-labelled probes (SK19, SK70 and SK31, respectively) and detected by PAGE-autoradiography. HIV-1 DNA was detected as follows. Asymptomatic patients: Monocytes– 61.5%, 100%, LTR 0%; lymphocytes– 100%, 92.3%, 53.84%. ARC patients: Monocytes– 71.4%, 57.1%, 0%; lymphocytes– 100%, 71.4%, 71.4%. AIDS patients: Monocytes– 80.0%, 100%, 0%; lymphocytes– 100%, 60%, 60%. The presence of HIV-1 DNA was confirmed in the monocyte fraction. In this cell subset, the gene-directed primers were the most effective for amplification, whereas the gene-directed primers failed to amplify HIV-1 DNA. The different pattern of amplification found in monocytes may suggest that these cells could be infected by a genetic variant of the virus.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error