The pheno- and genotypes of Shiga-like toxins (SLTs) in non-O157 strains from patients with haemolytic uraemic syndrome were determined. The clinical isolates investigated were from Italy and Germany and belonged to serotypes O22:H8, O26:H, O26:H11, O91:H, O111:H and O128:H; one isolate was non-typable. SLT genotypes were analysed by complete nucleotide sequence analysis of the B-subunit genes. The results showed that 14 strains possessed alone, two contained alone and five isolates harboured both and genes. In only two strains were -related genes found, together with either or . These findings indicate that variants of SLT-II are rarely found in non-O157 isolates from patients with haemolytic uraemic syndrome. Polymerase chain reaction (PCR) with cycle sequencing was found to be a suitable method for classification of genotypes.


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