1887

Abstract

Surmmary

The pheno- and genotypes of Shiga-like toxins (SLTs) in non-O157 strains from patients with haemolytic uraemic syndrome were determined. The clinical isolates investigated were from Italy and Germany and belonged to serotypes O22:H8, O26:H, O26:H11, O91:H, O111:H and O128:H; one isolate was non-typable. SLT genotypes were analysed by complete nucleotide sequence analysis of the B-subunit genes. The results showed that 14 strains possessed alone, two contained alone and five isolates harboured both and genes. In only two strains were -related genes found, together with either or . These findings indicate that variants of SLT-II are rarely found in non-O157 isolates from patients with haemolytic uraemic syndrome. Polymerase chain reaction (PCR) with cycle sequencing was found to be a suitable method for classification of genotypes.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-42-6-404
1995-06-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/42/6/medmicro-42-6-404.html?itemId=/content/journal/jmm/10.1099/00222615-42-6-404&mimeType=html&fmt=ahah

References

  1. Karmali M. A., Petrie M., Lim C., Fleming P. C., Arbus G. S., Lior H. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli . J Infect Dis 1985; 151:775–782
    [Google Scholar]
  2. Karch H., Heesemann J., Laufs R. Phage-associated cytotoxin production by and enteroadhesiveness of enteropathogenic Escherichia coli isolated from infants with diarrhea in West Germany. J Infect Dis 1987; 155:707–715
    [Google Scholar]
  3. Bitzan M., Moebius E., Ludwig K., Muller , Wiefel D. E., Heesemann J., Karch H. High incidence of serum antibodies to Escherichia coli O157 lipopolysaccharide in children with hemolytic-uremic syndrome. J Pediatr 1991; 119:380–385
    [Google Scholar]
  4. Caprioli A., Luzzi I., Rosmini F. Hemolytic-uremic syndrome and Vero cytotoxin-producing Escherichia coli infection in Italy. J Infect Dis 1992; 166:154–158
    [Google Scholar]
  5. Chart H., Smith H. R., Scotland S. M., Rowe B., Milford D. V., Taylor C. M. Serological identification of Escherichia coli O157:H7 infection in haemolytic uraemic syndrome. Lancet 1991; 337:138–140
    [Google Scholar]
  6. Scotland S. M., Rowe B., Smith H. R., Willshaw G. A., Gross R. J. Vero cytotoxin-producing strains of Escherichia coli from children with haemolytic uraemic syndrome and their detection by specific DNA probes. J Med Microbiol 1988; 25:237–243
    [Google Scholar]
  7. Beutin L., Montenegro M. A., Orskov I. Close association of verotoxin (Shiga-like toxin) production with enterohemolysin production in strains of Escherichia coli . J Clin Microbiol 1989; 27:2559–2564
    [Google Scholar]
  8. Bockemuhl J., Aleksic S., Karch H. Serological and biochemical properties of Shiga-like toxin (verocytotoxin)-producing strains of Escherichia coli, other than O-group 157, from patients in Germany. Int J Med Microbiol Virol Parasitol Infect Dis 1992; 276:189–195
    [Google Scholar]
  9. Karmali M. A. Infection by verocytotoxin-producing Escherichia coli . Clin Microbiol Rev 1989; 2:15–38
    [Google Scholar]
  10. Caprioli A., Luzzi I., Rosmini F. Communitywide outbreak of hemolytic-uremic syndrome associated with non-O157 verocytotoxin-producing Escherichia coli . J Infect Dis 1994; 169:208–211
    [Google Scholar]
  11. O’Brien A. D., Tesh V. L., Donohue Rolfe A. Shiga toxin: biochemistry, genetics, mode of action, and role in pathogenesis. Curr Top Microbiol Immunol 1992; 180:65–94
    [Google Scholar]
  12. Schmitt C. K., McKee M. L., O’Brien A. D. Two copies of Shiga-like toxin II-related genes common in enterohemorrhagic Escherichia coli strains are responsible for the antigenic heterogeneity of the O157: H strain E3 2511. Infect Immun 1991; 59:1065–1073
    [Google Scholar]
  13. Meyer T., Karch H., Hacker J., Bocklage H., Heesemann J. Cloning and sequencing of a Shiga-like toxin II-related gene from Escherichia coli O157: H7 strain 7279. Int J Med Microbiol Virol Parasitol Infect Dis 1992; 276:176–188
    [Google Scholar]
  14. Gannon V. P., Teerling C., Masri S. A., Gyles C. L. Molecular cloning and nucleotide sequence of another variant of the Escherichia coli Shiga-like toxin II family. J Gen Microbiol 1990; 36:1125–1135
    [Google Scholar]
  15. Ito H., Terai A., Kurazono H., Takeda Y., Nishibuchi M. Cloning and nucleotide sequencing of Vero toxin 2 variant genes from Escherichia coli O91: H21 isolated from a patient with the hemolytic uremic syndrome. Microb Pathog 1990; 8:47–60
    [Google Scholar]
  16. Paton A. W., Paton J. C., Heuzenroeder M. W., Goldwater P. N., Manning P. A. Cloning and nucleotide sequence of a variant Shiga-like toxin II gene from Escherichia coli OX3:H21 isolated from a case of sudden infant death syndrome. Microb Pathog 1992; 13:225–236
    [Google Scholar]
  17. Paton A. W., Paton J. C., Manning P. A. Polymerase chain reaction amplification, cloning and sequencing of variant Escherichia coli Shiga-like toxin type II operons. Microb Pathog 1993; 15:77–82
    [Google Scholar]
  18. Rüssmann H., Schmidt H., Heesemann J., Caprioli A., Karch H. Variants of Shiga-like toxin II constitute a major toxin component in Escherichia coli O157 strains from patients with haemolytic uraemic syndrome. J Med Microbiol 1994; 40:338–343
    [Google Scholar]
  19. Bitzan M., Karch H., Maas M. G. Clinical and genetic aspects of Shiga-like toxin production in traditional enteropathogenic Escherichia coli . Int J Med Microbiol 1991; 274:496–506
    [Google Scholar]
  20. Bockemühl J., Karch H., Rüssmann H., Aleksic S., WiB R., Emmrich P. Transmission of Shiga-like toxin (Verotoxin)-producing Escherichia coli O22:H8 by unpasteurized milk followed by a case of haemolytic-uraemic syndrome. Bundesgesundheitsblatt 1990; 1:3–6
    [Google Scholar]
  21. Farina C., Gavazzeni G., Caprioli A., Remuzzi G. Hemolytic uremic syndrome associated with verocytotoxin-producing Escherichia coli infection in acquired immunodeficiency syndrome. Blood 1990; 75:2465
    [Google Scholar]
  22. Ricotti G. C., Buonomini M. I., Merlitti A., Karch H., Luzzi I., Caprioli A. A fatal case of hemorrhagic colitis, thrombocytopenia, and renal failure associated with verocytotoxin-producing, non O157 Escherichia coli . Clin Infect Dis 1994; 19:815–816
    [Google Scholar]
  23. Stenger K. O., Windier F., Karch H., von Wulffen H., Heesemann J. Hemolytic-uremic syndrome associated with an infection by verotoxin producing Escherichia coli O111 in a woman on oral contraceptives. Clin Nephrol 1988; 29:153–158
    [Google Scholar]
  24. Karch H., Meyer T. Evaluation of oligonucleotide probes for identification of shiga-like-toxin-producing Escherichia coli . J Clin Microbiol 1989; 27:1180–1186
    [Google Scholar]
  25. Schmidt H., Montag M., Bockemühl J., Heesemann J., Karch H. Shiga-like toxin II-related cytotoxins in Citrobacterfreundii strains from humans and beef samples. Infect Immun 1993; 61:534–543
    [Google Scholar]
  26. Thomas A., Smith H. R., Rowe B. Use of digoxigenin-labelled oligonucleotide DNA probes for VT2 and VT2 human variant genes to differentiate vero cytotoxin-producing Escherichia coli strains of serogroup O157.7. Clin Microbiol 1993; 31:1700–1703
    [Google Scholar]
  27. Tyler S. D., Johnson W. M., Lior H., Wang G., Rozee K. R. Identification of verotoxin type 2 variant B subunit genes in Escherichia coli by the polymerase chain reaction and restriction fragment length polymorphism analysis. J Clin Microbiol 1991; 29:1339–1343
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-42-6-404
Loading
/content/journal/jmm/10.1099/00222615-42-6-404
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error