1887

Abstract

Surmmary

The detection of was investigated in an in-vitro system consisting of a human lung epithelial cell line (A-549) inoculated with infected peripheral blood mononuclear cells (PMBC) from HIV-infected patients with proven or suspected pneumonia (PCP), and from HIV-negative patients with other lung infections. Supernates from cultures were sampled daily and evaluated for the presence of by Giemsa and immunofluorescence staining. was isolated from 98 (95.1%) of 103 culture supernate samples from patients with proven pneumocystosis and 45 rvival for up to 3 weeks was observed. Recovery of from infected PBMC strongly supports previous observations about its ability to disseminate haematogenously and could represent a further advance in understanding the pathogenesis and diagnosis of PCP.

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1995-06-01
2022-01-22
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References

  1. Lipschik G. Y., Gill V. J., Lundgren J. D. Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood. Lancet 1992; 340:203–206
    [Google Scholar]
  2. Schluger N., Godwin T., Sepkowitz K. Application of DNA amplification to pneumocystosis; presence of serum Pneumocystis carinii DNA during human and experimentally induced Pneumocystis carinii pneumonia. J Exp Med 1992; 176:1327–1333
    [Google Scholar]
  3. Sepkowitz K., Schluger N., Godwin T., Armstrong D., Cerami A., Bucala R. DNA amplification in experimental pneumocystosis: characterization of serum Pneumocystis carinii DNA and potential P. carinii carrier states. J Infect Dis 1993; 168:421–426
    [Google Scholar]
  4. Wakefield A. E., Pixley F. J., Banerji S. Detection of Pneumocystis carinii with DNA amplification. Lancet 1990; 336:451–453
    [Google Scholar]
  5. Limper A. H. Parasitic adherence and host responses in the development of Pneumocystis carinii pneumonia. Semin Respir Infect 1991; 6:19–26
    [Google Scholar]
  6. Pesanti E. L. Interaction of cytokines and alveolar cells with Pneumocystis carinii in vitro. J Infect Dis 1991; 163:611–613
    [Google Scholar]
  7. Cushion M. T., Walzer P. D. Growth and serial passage of Pneumocystis carinii in the A549 cell line. Infect Immun 1984; 44:245–251
    [Google Scholar]
  8. Walker J., Conner G., Ho J., Hunt C., Pickering L. Giemsa staining for cysts and trophozoites of Pneumocystis carinii . J Clin Pathol 1989; 42:432–434
    [Google Scholar]
  9. Nato F., Contini C., Zamora Zavala C. Production and characterization of monoclonal antibodies to human Pneumocystis carinii for the diagnosis of P. carinii pneumonia. Eur J Med 1992; 1:132–138
    [Google Scholar]
  10. Jackson P. R., Pappas M. G., Hansen B. D. Fluorogenic substrate detection of viable intracellular and extracellular pathogenic protozoa. Science 1985; 227:435–438
    [Google Scholar]
  11. Walzer P. D., Rutledge M. E., Yoneda K., Stahr B. J. Pneumocystis carinii: new separation method from lung tissue. Exp Parasitol 1979; 47:356–368
    [Google Scholar]
  12. Lennette E. H. (ed) Manual of clinical microbiology 3rd edn Washington, DC: American Society for Microbiology; 1980150–159 533–540, 760–761
    [Google Scholar]
  13. Maddison S. E., Hayes G. V., Slemenda S. B., Normal L. G., Ivey M. H. Detection of specific antibody by enzyme-linked immunosorbent assay and antigenemia by counterimmunoelectro-phoresis in humans infected with Pneumocystis carinii . J Clin Microbiol 1982; 15:1036–1043
    [Google Scholar]
  14. McNabb S. J. N., Graves D. C., Kosanke S. D., Moyer M. J., Ivey M. H. Pneumocystis carinii antigen detection in rat serum and lung lavage. J Clin Microbiol 1988; 26:1763–1771
    [Google Scholar]
  15. Raviglione M. C. Extrapulmonary pneumocystosis. The first 50 cases. Rev Infect Dis 1990; 12:1127–1138
    [Google Scholar]
  16. Contini C., Romani R., Vullo V., Delia S., Sorice F. Detection of Pneumocystis carinii in urinary samples from a patient with AIDS despite treatment with aerosolized pentamidine. Clin Infect Dis 1994; 18:477–178
    [Google Scholar]
  17. Sloand E., Laughon B., Armstrong M. The challenge of Pneumocystis carinii culture. J Eukaryot Microbiol 1993; 40:188–195
    [Google Scholar]
  18. Contini C., Manganaro M., Romani R. Activity of terbinafine against Pneumocystis carinii in vitro and its efficacy in the treatment of experimental pneumonia. J Antimicrob Chemother 1994; 34:727–735
    [Google Scholar]
  19. Masur H., Jones T. C. The interaction in vitro of Pneumocystis carinii with macrophages and L-cells. J Exp Med 1978; 147:157–170
    [Google Scholar]
  20. Pottratz S. T., Paulsrud J., Smith J. S., Martin W. J. Pneumocystis carinii attachment to cultured lung cells by Pneumocystis gp 120, a fibronectin binding protein. J Clin Invest 1991; 88:403–407
    [Google Scholar]
  21. Koziel H., Baik J., Armstrong M. J. K., Rose R. M. Pneumocystis carinii glycoprotein-A is a chemotactic factor for normal human blood monocytes. Am Rev Respir Dis 1993; 147:33 (abstract)
    [Google Scholar]
  22. Blumenfeld W., Grifiss J. M. In vitro differentiation of human-derived Pneumocystis carinii. J Clin Microbiol 1989; 27:480–485
    [Google Scholar]
  23. Armstrong M. Y. K., Richards F. F. Propagation and purification of rat Pneumocystis carinii in short-term culture. J Protozool 1989; 36:24S–27S
    [Google Scholar]
  24. Lee C. H., Bauer N. L., Shaw M. M. Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks. J Clin Microbiol 1993; 31:1659–1662
    [Google Scholar]
  25. Hong S.-T., Steele P. E., Cushion M. T., Walzer P. D., Stringer S. L., Stringer J. R. Pneumocystis carinii karyotypes. J Clin Microbiol 1990; 28:1785–1795
    [Google Scholar]
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