1887

Abstract

Surmmary

Fifty-seven isolates of were obtained from different sites within the oral cavities of 18 dental patients without AIDS or any malignancies. Eleven of the patients had oral candidosis associated with the wearing of dentures. The genotypic relationships of the individual isolates were determined by hybridisation of a -specific moderately repetitive sequence, 27A, to RI-digested chromosomal DNA. From the DNA profiles, the isolates could be divided into 22 distinct genetic groups. In the majority of patients, a single unique strain of appeared to dominate in the oral cavity. Re-infection following antifungal therapy was generally due to the re-emergence of the original infecting strain. The strains isolated from dental plates did not form a distinct genetic group. These results suggest that denture stomatitis is due to the outgrowth of commensal strains of .

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1995-05-01
2024-04-24
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References

  1. Arendorf T. M., Walker D. M. The prevalence and intra-oral distribution of Candida albicans in man. Arch Oral Biol 1980; 25:1–10
    [Google Scholar]
  2. Welch K., Finkbeiner W., Alpers C. E. Autopsy findings in the acquired immuno-deficiency syndrome. JAMA 1984; 252:1151–1159
    [Google Scholar]
  3. Odds F. C. Candida and candidosis. 2nd edn London: Bailliere Tindall; 1988468
    [Google Scholar]
  4. Kirkpatrick C. H. Chronic mucocutaneous candidiasis. Eur J Clin Microbiol Infect Dis 1989; 8:448–456
    [Google Scholar]
  5. Schmid J., Odds F. C., Wiselka M. J., Nicholson K. G., Soll D. R. Genetic similarity and maintenance of Candida albicans strains from a group of AIDS patients, demonstrated by DNA fingerprinting. J Clin Microbiol 1992; 30:935–941
    [Google Scholar]
  6. Schmid J., Rotman M., Reed B., Pierson C. L., Soll D. R. Genetic similarity of Candida albicans strains from vaginitis patients and their partners. J Clin Microbiol 1993; 31:39–46
    [Google Scholar]
  7. Hellstein J., Vawter-Hugart H., Fotos P., Schmid J., Soll D. R. Genetic similarity and phenotypic diversity of commensal and pathogenic strains of Candida albicans isolated from the oral cavity. J Clin Microbiol 1993; 31:3190–3199
    [Google Scholar]
  8. Whelan W. L., Kirsch D. R., Kwon-Chung K. J., Wahl S. M., Smith P. D. Candida albicans in patients with the acquired immunodeficiency syndrome: absence of a novel or hypervirulent strain. J Infect Dis 1990; 162:513–518
    [Google Scholar]
  9. Soll D. R., Galask R., Schmid J., Hanna C., Mac K., Morrow B. Genetic dissimilarity of commensal strains of Candida spp. carried in different anatomical locations of the same healthy women. J Clin Microbiol 1991; 29:1702–1710
    [Google Scholar]
  10. Scherer S., Stevens D. A. A Candida albicans dispersed, repeated gene family and its epidemiologic applications. Proc Natl Acad Sci USA 1988; 85:1452–1456
    [Google Scholar]
  11. Mathaba L. T., Franklyn K. M., Warmington J. R. A rapid technique for the isolation of DNA from clinical isolates of Candida albicans . J Microbiol Methods 1993; 17:17–26
    [Google Scholar]
  12. Reed K. C., Mann D. A. Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res 1985; 13:7207–7221
    [Google Scholar]
  13. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 1983, 132, 6–13. Addendum: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 1984; 137:266–267
    [Google Scholar]
  14. Felsenstein J. PHYLIP (Phylogeny Inference Package) Version 3.2p. Cladistics 1989; 5:164–166
    [Google Scholar]
  15. McCullough M. J., Ross B. C., Dwyer B. D., Reade P. C. Genotype and phenotype of oral Candida albicans from patients infected with the human immunodeficiency virus. Microbiologica 1994; 140:1195–1202
    [Google Scholar]
  16. Pittet D., Monod M., Filthuth I., Frenk E., Suter P. M., Auckenthaler R. Contour-clamped homogeneous electric field gel electrophoresis as a powerful epidemiologic tool in yeast infections. Am J Med 1991; 91:(3B)256S–263S
    [Google Scholar]
  17. Millon L., Manteaux A., Reboux G. Fluconazole-resistant recurrent oral candidiasis in human immunodeficiency virus-positive patients: persistence of Candida albicans strains with the same genotype. J Clin Microbiol 1994; 32:1115–1118
    [Google Scholar]
  18. Soll D. R., Staebell M., Langtimm C., Pfaller M., Hicks J., Rao T. V. G. Multiple Candida strains in the course of a single systemic infection. J Clin Microbiol 1988; 26:1448–1459
    [Google Scholar]
  19. Martin M. V., Craig G. T., Lamb D. J. An investigation of the role of true hypha production in the pathogenesis of experimental oral candidosis. Sabouraudia 1984; 22:471–476
    [Google Scholar]
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