1887

Abstract

Surmmary

A combined reverse transcription-polymerase chain reaction (RT-PCR) method was employed for the detection of hepatitis C virus (HCV) RNA in serum from patients with chronic active hepatitis, with primers corresponding to the 5′ non-coding region. The diagnosis was based on serological and biochemical methods and on liver biopsy. HCV-RNA was detected in 27 (90%) of 30 sera examined. The nucleotide sequence of PCR-amplified HCV cDNAs (256 bp) was determined from five specimens and heterogeneity varying between 0.58% and 2.89% among the clinical samples and the prototype HCV-1 was found.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-42-5-367
1995-05-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/42/5/medmicro-42-5-367.html?itemId=/content/journal/jmm/10.1099/00222615-42-5-367&mimeType=html&fmt=ahah

References

  1. Choo Q.-L., Kuo G., Weiner A. J., Overby L. R., Bradley D. W., Houghton M. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 1989; 244:359–362
    [Google Scholar]
  2. Kuo G., Choo Q.-L., Alter H. J. An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science 1989; 244:362–364
    [Google Scholar]
  3. Alter H. J., Purcell R. H., Shih J. W. Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute chronic non-A, non-B hepatitis. N Engl J Med 1989; 321:1494–1500
    [Google Scholar]
  4. Choo Q.-L., Richman K. H., Han J. H. Genetic organization and diversity of the hepatitis C virus. Proc Natl Acad Sci USA 1991; 88:2451–2455
    [Google Scholar]
  5. Kato N., Hijikata M., Ootsuyama Y. Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proc Natl Acad Sci USA 1990; 87:9524–9528
    [Google Scholar]
  6. Okamoto H., Okada S., Sugiyama Y. Nucleotide sequence of the genomic RNA of hepatitis C virus isolated from a human carrier: comparison with reported isolates for conserved and divergent regions. J Gen Virol 1991; 72:2697–2704
    [Google Scholar]
  7. Takamizawa A., Mori C., Fuke I. Structure and organization of the hepatitis C virus genome isolated from human carriers. J Virol 1991; 65:1105–1113
    [Google Scholar]
  8. Chen P. J., Lin M. H., Tai K. F., Liu P. C., Lin C.-J., Chen D. S. The Taiwanese hepatitis C virus genome: sequence determination and mapping the 5′ termini of viral genomic and antigenomic RNA. Virology 1992; 188:102–113
    [Google Scholar]
  9. Okamoto H., Kurai K., Okada S. Full-length sequence of a hepatitis C virus genome having poor homology to reported isolates: comparative study of four distinct genotypes. Virology 1992; 188:331–341
    [Google Scholar]
  10. Han J. H., Shyamala V., Richman K. H. Characterization of the terminal regions of hepatitis C viral RNA: Identification of conserved sequences in the 5′ untranslated region and poly (A) tails at the 3′ end. Proc Natl Acad Sci USA 1991; 88:1711–1715
    [Google Scholar]
  11. Bukh J., Purcell R. H., Miller R. H. Sequence analysis of the 5′ noncoding region of hepatitis C virus. Proc Natl Acad Sci USA 1991; 89:4942–4946
    [Google Scholar]
  12. Chan S.-W., McOmish F., Holmes E. C. Analysis of a new hepatitis C virus type and its phylogenetic relationship to existing variants. J Gen Virol 1992; 73:1131–1141
    [Google Scholar]
  13. Simmonds P., McOmish F., Yap P. L. Sequence variability in the 5′ non-coding region of hepatitis C virus: identification-of a new virus type and restrictions on sequence diversity. J Gen Virol 1993; 74:661–668
    [Google Scholar]
  14. Simmonds P., Holmes E. C., Cha T. A. Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region. J Gen Virol 1993; 74:2391–2399
    [Google Scholar]
  15. Simmonds P., Smith D. B., McOmish F. Identification of genotypes of hepatitis C virus by sequence comparisons in the core, El and NS-5 regions. J Gen Virol 1994; 75:1053–1061
    [Google Scholar]
  16. Bukh J., Purcell R., Miller R. H. At least 12 genotypes of hepatitis C virus predicted by sequence analysis of the putative El gene isolates collected worldwide. Proc Natl Acad Sci USA 1993; 90:8234–8238
    [Google Scholar]
  17. Houghton M., Weiner A., Han J., Kuo G., Choo Q.-L. Molecular biology of the hepatitis C viruses: implications for diagnosis, development and control of viral disease. Hepatology 1991; 14:381–388
    [Google Scholar]
  18. Mori S., Kato N., Yagyu A. A new type of hepatitis C virus in patients in Thailand. Biochem Biophys Res Commun 1992; 183:334–342
    [Google Scholar]
  19. Myers T. W., Gelfand D. H. Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry 1991; 30:7661–7666
    [Google Scholar]
  20. Young K. K. Y., Resnick R. M., Myers T. W. Detection of the hepatitis C virus RNA by a combined reverse transcription-polymerase chain reaction assay. J Clin Microbiol 1993; 31:882–886
    [Google Scholar]
  21. Busch M. P., Wilber J. C., Johnson P., Tobler L., Evans C. S. Impact of specimen handling and storage on hepatitis C virus RNA. Transfusion 1992; 32:420–425
    [Google Scholar]
  22. Bukh J., Purcell R. H., Miller R. H. Importance of primer selection for the detection of hepatitis C virus RNA with the polymerase chain reaction assay. Proc Natl Acad Sci USA 1992; 89:187–191
    [Google Scholar]
  23. McOmish F., Yap P. L., Dow B. C. Geographical distribution of hepatitis C virus genotypes in blood donors: an international collaborative survey. J Clin Microbiol 1994; 32:884–892
    [Google Scholar]
  24. Stuyver L., Rossau R., Wyseur A. Typing of hepatitis C virus isolates and characterization of new subtypes using a line probe assay. J Gen Virol 1993; 74:1093–1102
    [Google Scholar]
  25. Nei M. (ed) Molecular evolutionary genetics New York: Columbia University Press; 198764–110
    [Google Scholar]
  26. Brown E. A., Zhang H., Ping L.-H., Lemon S. M. Secondary structure of the 5′ nontranslated regions of hepatitis C virus and pestivirus genomic RNAs. Nucleic Acids Res 1992; 20:5041–5045
    [Google Scholar]
  27. Tsukiyama-Kohara K., Iizuka N., Kohara M., Nomoto A. Internal ribosome entry site within hepatitis C virus RNA. J Virol 1992; 66:1476–1483
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-42-5-367
Loading
/content/journal/jmm/10.1099/00222615-42-5-367
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error