Eight leptospiral and three non-leptospiral primers were examined in the development of a random amplified polymorphic DNA (RAPD) fingerprinting method for identification of leptospiral serovars within the serogroup Sejroe. The strains examined comprised 19 reference strains of serogroup Sejroe, five field strains and 17 Leptospira borgpetersenii serovar hardjo type hardjobovis strains. Unique DNA banding patterns were obtained for each of the 17 reference strains with L. hardjo type hardjobovis-derived primers, and all clinical isolates were identified as L. hardjo type hardjobovis. Although the profiles were specific for each serovar, most serovars within serogroup Sejroe clustered into previously recognised genetic groups. RAPD fingerprinting is a simple, rapid method suitable for the identification of some Leptospira serovars.
TerpstraW. J.,
KorverH.,
Van LeeuwenJ.,
KlatserP. R.,
KolkA. H. The classification of Sejroe group serovars of Leptospira interrogans with monoclonal antibodies. Zentralbl Bakteriol Mikrobiol Hyg A1985; 259:498–506
EllisW. A.,
MontgomeryJ. M.,
ThiermannA. B. Restriction endonuclease analysis as a taxonomic tool in the study of pig isolates belonging to the Australis serogroup of Leptospira interrogans
. J Clin Microbiol1991; 29:957–961
HookeyJ. V.,
WaitkinsS. A.,
GahirR. The use of bacterial restriction endonuclease analysis to classify icterohaemorrhagiae and autumnalis serovars. Isr J Vet Med1987; 43:265–270
TerpstraW. J.,
KorverH.,
SchooneG. J. Comparative classification of leptospira serovars of the Pomona group by monoclonal antibodies and restriction endonuclease analysis. Zentralbl Bakteriol Mikrobiol Hyg A1978; 266:412–421
PerolatP.,
LecuyerI.,
PosticD.,
BarantonG. Diversity of ribosomal DNA fingerprints of Leptospira serovars provides a database for subtyping and species assignation. Res Microbiol1993; 144:5–15
RamadassP.,
JarvisB. D. W.,
CornerR. J.,
PennyD.,
MarshallR. B. Genetic characterization of pathogenic Leptospira species by DNA hybridization. Int J Syst Bacteriol1992; 42:215–219
Van EysG. J. J. M.,
GerritsenM. J.,
KorverH.,
SchooneG. J.,
KroonC. C.,
TerpstraW. J. Characterization of serovars of the genus Leptospira by DNA hybridization with hardjobovis and icterohaemorrhagiae recombinant probes with special attention to serogroup Sejroe. J Clin Microbiol1991; 29:1042–1048
YasudaP. H.,
SteigerwaltA. G.,
SulzerK. R.,
KaufmannA. F.,
RogersF.,
BrennerD. J. Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species. Int J Syst Bacteriol1987; 37:407–415
ZuernerR. L.,
BolinC. A. Nucleic acid probe characterizes Leptospira interrogans serovars by restriction fragment length polymorphisms. Vet Microbiol1990; 24:355–366
ZuernerR. L.,
EllisW. A.,
BolinC. A.,
MontgomeryJ. M. Restriction fragment length polymorphisms distinguish Leptospira borgpetersenii serovar hardjo type hardjo-bovis isolates from different geographical locations. J Clin Microbiol1993; 31:578–583
HermannJ. L.,
BellengerE.,
PerolatP.,
BarantonG.,
Saint GironsI. Pulsed-field gel electrophoresis of Not digests of leptospiral DNA: a new rapid method of serovar identification. J Clin Microbiol1992; 30:1696–1702
WoodwardM. J.,
SullivanG. J.,
PalmerN. M. A.,
WoolleyJ. C.,
RedstoneJ. S. Development of a PCR test specific for Leptospira hardjo genotype bovis
. Vet Rec1991; 128:282–283
CorneyB. G.,
ColleyJ.,
DjordjevicS. P.,
WhittingtonR.,
GrahamG. C. Rapid identification of some Leptospira isolates from cattle by random amplified polymorphic DNA fingerprinting. J Clin Microbiol1993; 31:2927–2932
ThiermannA. B.,
HandsakerA. L.,
FoleyJ. W.,
WhiteF. H.,
KingscoteB. F. Reclassification of North American leptospiral isolates belonging to serogroups Mini and Sejroe by restriction endonuclease analysis. Am J Vet Res1986; 47:61–66
GravekampC.,
Van de KempH.,
FranzenM. Detection of seven species of pathogenic leptospiras by PCR using two sets of primers. J Gen Microbiol1993; 139:1691–1700
MerienF.,
AmouriauxP.,
PerolatP.,
BarantonG.,
Saint GironsI. Polymerase chain reaction for the detection of Leptospira spp. in clinical samples. J Clin Microbiol1992; 30:2219–2224
Van BelkumA.,
StruelensM.,
QuintW. Typing of Legionella pneumophila strains by polymerase chain reaction-mediated DNA fingerprinting. J Clin Microbiol1993; 31:2198–2200
GiesendorfB. A. J.,
van BelkumA.,
KoekenA. Development of species-specific DNA probes for Campylobacter jejuni, Campylobacter coli, and Campylobacter lari by polymerase chain reaction fingerprinting. J Clin Microbiol1993; 31:1541–1546
WoodwardM. J.,
RedstoneJ. S. Differentiation of leptospira serovars by the polymerase chain reaction and restriction fragment length polymorphism. Vet Rec1993; 132:325–326
GerritsenM. J.,
KoopmansM. J.,
OlyhoekT. Effect of streptomycin treatment on the shedding of and the serologic responses to Leptospira interrogans serovar hardjo subtype hardjobovis in experimentally infected cows. Vet Microbiol1993; 38:129–135