1887

Abstract

Surmmary

A possible relationship between the ability of strains to invade tissues and genetic polymorphism was studied by random amplified polymorphic DNA (RAPD) analysis. One hundred randomly designed oligonucleotide decamers were examined with DNA of three reference strains, eight environmental isolates and 21 isolates from two distinct clinical situations: Non-invasive aspergillosis (predominantly aspergilloma) and invasive aspergillosis. One primer (OPQ 6) was found to generate a reproducible amplification product that enabled distinction between the two groups according to the presence or absence of a 0.95-kb fragment that correlated with the nature of the infection (non-invasive or invasive) and immune status of the patient. The results indicated that the pathogenicity of was related not only to the host’s immune status but also to the virulence of the strain of .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-42-4-299
1995-04-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/42/4/medmicro-42-4-299.html?itemId=/content/journal/jmm/10.1099/00222615-42-4-299&mimeType=html&fmt=ahah

References

  1. Kolattukudy P. E., Lee J. D., Rogers L. M., Zimmerman P. Evidence for possible involvement of an elastolytic serine protease in aspergillosis. Infect Immun 1993; 61:2357–2368
    [Google Scholar]
  2. Monod M., Paris S., Sarfati J., Jaton-Ogay K., Ave P., Latgé J. P. Virulence of alkaline protease-deficient mutants of Aspergillus fumigatus . FEMS Microbiol Lett 1993; 106:39–46
    [Google Scholar]
  3. Markaryan A., Morozova I., Yu H., Kolattukudy P. E. Purification and characterization of an elastolytic metallo-protease from Aspergillus fumigatus and immunoelectron microscopic evidence of secretion of this enzyme by the fungus invading the murine lung. Infect Immun 1994; 62:2149–2157
    [Google Scholar]
  4. Smith J. M., Davies J. E., Holden D. W. Construction and pathogenicity of Aspergillus fumigatus mutants that do not produce the ribotoxin restrictocin. Mol Microbiol 1993; 9:1071–1077
    [Google Scholar]
  5. Bumie J. P., Coke A., Matthews R. C. Restriction endonuclease analysis of Aspergillus fumigatus DNA. J Clin Pathol 1992; 45:324–327
    [Google Scholar]
  6. Denning D. W., Clemons K. L., Hanson L. H., Stevens D. A. Restriction endonuclease analysis of total cellular DNA of Aspergillus fumigatus isolates of geographically and epidemiologically diverse origin. J Infect Dis 1990; 162:1151–1158
    [Google Scholar]
  7. Williams J. G. K., Kubelik A. R., Livak K. J., Rafalski J. A. Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990; 18:6531–6535
    [Google Scholar]
  8. Goodwin P. H., Annis S. L. Rapid identification of genetic variation and pathotype of Leptosphaeria maculans by random amplified polymorphic DNA assay. Appl Environ Microbiol 1991; 57:2482–2486
    [Google Scholar]
  9. Crowhurst R. N., Hawthorne B. T., Rikkerink E. H. A., Templeton M. D. Differentiation of Fusarium solani f. sp. cucurbitae races 1 and 2 by random amplification of polymorphic DNA. Curr Genet 1991; 20:391–396
    [Google Scholar]
  10. Bodey G. P., Vartivarian S. Aspergillosis. Eur J Microbiol Infect Dis 1989; 8:413–437
    [Google Scholar]
  11. Cenis J. L. Rapid extraction of fungal DNA for PCR amplification. Nucleic Acids Res 1992; 20:2380
    [Google Scholar]
  12. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 1977; 74:5463–5467
    [Google Scholar]
  13. Aufaure-Brown A., Cohen J., Holden D. W. Use of randomly amplified polymorphic DNA markers to distinguish isolates of Aspergillus fumigatus . J Clin Microbiol 1992; 30:2991–2993
    [Google Scholar]
  14. Van Belkum A., Quint W. G. V., De Pauw B. E., Melchers W. J. G., Meis J. F. Typing of Aspergillus species and Aspergillus fumigatus isolates by interrepeat polymerase chain reaction. J Clin Microbiol 1993; 31:2502–2505
    [Google Scholar]
  15. Loudon K. W., Bumie J. P., Coke A. P., Matthews R. C. Application of polymerase chain reaction to fingerprinting Aspergillus fumigatus by random amplification of polymorphic DNA. J Clin Microbiol 1993; 31:1117–1121
    [Google Scholar]
  16. Girardin H., Sarfati J., Traoré F., Dupouy-Carnet J., Derouin F., Latgé J. P. Molecular epidemiology of nosocomial invasive aspergillosis. J Clin Microbiol 1994; 32:684–690
    [Google Scholar]
  17. Denning D. W. Epidemiology and pathogenesis of systemic fungal infections in the immunocompromised host. J Antimicrob Chemother 1991; 28: Suppl B 1–16
    [Google Scholar]
  18. Tang C. M., Cohen J., Rees A. J., Holden D. W. Molecular epidemiological study of invasive pulmonary aspergillosis in renal transplantation unit. Eur J Microbiol Infect Dis 1994; 13:318–321
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-42-4-299
Loading
/content/journal/jmm/10.1099/00222615-42-4-299
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error