Vibrio cholerae O139 organisms isolated from different parts of India and from Bangladesh were characterised with respect to their haemagglutination (HA) activity, plasmid content, cholera toxin (CT) production, cell surface protein and lipopolysaccharide (LPS) profiles, and antigenic properties. Of 28 V. cholerae O139 isolates tested, 14 (50%) were shown to agglutinate chicken erythrocytes; the HA activity was sensitive to D-mannose 0.1 %. In parallel experiments, 12 (92.3%) of 13 V. cholerae O1 (El Tor) and 12 (75%) of 16 non-O1, non-O139 strains agglutinated chicken erythrocytes. Plasmid analysis of 32 O139 isolates showed that 12 (37.5%) carried one or more plasmids of 35.8-2.6 MDa. Plasmids were not detected in any of the V. cholerae O1 strains, although plasmids were demonstrable in 35% of the non-O1, non-O139 strains tested. V. cholerae O139 isolates showed an ability to produce CT that depended on media composition and other cultural conditions. A comparison of envelope and outer-membrane protein profiles between O1 and O139 isolates failed to show any significant differences. LPS analysis of O139 isolates revealed that these organisms were devoid of long “O” side-chain polysaccharides. Some of the non-O1, non-O139 strains also showed similar LPS profiles whereas others showed the presence of long repetitive “O” side-chain polysaccharides similar to those seen in O1 organisms. An antiserum raised against V. cholerae O1 strain O395 did not show any significant reactivity towards O139 and non-O1, non-O139 strains although it reacted with other O1 strains. Furthermore, the anti-O1 serum induced marked protection against challenge with an O1 strain but not with an O139 strain in passive protection experiments. All these results indicate that, despite sharing some common properties with V. cholerae O1, the O139 isolates posses certain characteristics that make them distinct from their O1 counterparts.
BlakeP. A. Historical perspectives on pandemic cholera. In
WachsmuthI. K.,
BlakeP. A.,
OlsvikO.
(eds) Vibrio cholerae and cholera: molecular to global perspectives Washington D. C.: ASM Press; 1994293
SanyalS. C. Epidemiology and pathogenicity of non-Ol vibrio species and related organisms. In
BaruaD.,
GreenoughW. B.
(eds) Cholera New York: Plenum Medical Book Company; 199257–67
RamamurthyT.,
GargS.,
SharmaR. Emergence of novel strain of Vibrio cholerae with epidemic potential in southern and eastern India. Lancet1993; 341:703–704
Cholera working group, International Centre for Diarrhoeal Disease Research, Bangladesh Large epidemic of choleralike disease in Bangladesh caused by Vibrio cholerae 0139 synonym Bengal. Lancet1993; 342:387–390
WaldorM. K.,
MekalanosJ. J. ToxR regulates virulence gene expression in non-Ol strains of Vibrio cholerae that cause epidemic cholera. Infect Immun1994; 62:72–78
SenguptaT. K.,
SenguptaD. K.,
NairG. B.,
GhoseA. C. Epidemic isolates of Vibrio cholerae 0139 express antigenically distinct types of colonization pili. FEMS Microbiol Lett1994; 118:265–272
SenguptaD.,
Datta-RoyK.,
BanerjeeK.,
GhoseA. C. Identification of some antigenically related outer-membrane proteins of strains of Vibrio cholerae 01 and non-Ol serovars involved in intestinal adhesion and the protective role of antibodies to them. J Med Microbiol1989; 29:33–39
Datta-RoyK.,
BanerjeeK.,
DeS. P.,
GhoseA. C. Comparative study of expression of hemagglutinins, hemolysins, and enterotoxins by clinical and environmental isolates of non-Ol Vibrio cholerae in relation to their enteropathogenicity. Appl Environ Microbiol1986; 52:875–879
FinkelsteinR. A.,
MukerjeeS. Hemagglutination: a rapid method for differentiating Vibrio cholerae and El Tor vibrios. Proc Soc Exp Biol Med1963; 112:355–359
Datta-RoyK.,
DasguptaC.,
GhoseA. C. Hemagglutination and intestinal adherence properties of clinical and environmental isolates of non-Ol Vibrio cholerae
. Appl Environ Microbiol1989; 55:2403–2406
BoothB. A.,
FinkelsteinR. A. Presence of hemagglutinin/protease and other potential virulence factors in Ol and non-Ol Vibrio cholerae
. J Infect Dis1986; 154:183–186
WestphalO.,
JannK. Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. Methods Carbohydr Chem1965; 5:83–91
SenguptaD. K.,
SenguptaT. K.,
GhoseA. C. Major outer membrane proteins of Vibrio cholerae and their role in induction of protective immunity through inhibition of intestinal colonization. Infect Immun1992; 60:4848–4855
FilipC.,
FletcherG.,
WulffJ. L.,
EarhartC. F. Solubilization of the cytoplasmic membrane of Escherichia coli by ionic detergent sodium-lauryl sarcosinate. J Bacteriol1973; 115:717–722
HolmgrenJ. Comparison of the tissue receptors for Vibrio cholerae and Escherichia coli enterotoxins by means of gangliosides and natural cholerae toxoid. Infect Immun1973; 8:851–859
SenguptaD. K.,
SenguptaT. K.,
GhoseA. C. Antibodies to outer membrane proteins of Vibrio cholerae induce protection by inhibition of intestinal colonization of vibrios. FEMS Microbiol Immunol1992; 89:261–266
HolmgrenJ.,
SvennerholmA.-M.,
LindbladM. Receptor-like glycocompounds in human milk that inhibit classical and El Tor Vibrio cholerae cell adherence (hemagglutination). Infect Immun1983; 39:147–154
CookW. L.,
WachsmuthK.,
JohnsonS. R.,
BirknessK. A.,
SamadiA. R. Persistence of plasmids, cholera toxin genes, and profage DNA in classical Vibrio cholerae Ol. Infect Immun1984; 45:222–226
BartowskyE. J.,
ManningP. A. Molecular donning of the plasmids of Vibrio cholerae 01 and the incidence of related plasmids in clinical isolates and other Vibrio species. FEMS Microbiol Lett1988; 50:183–190
BarjaJ. L.,
SantosY.,
HuqI.,
ColwellR. R.,
ToranzoA. E. Plasmids and factors associated with virulence in environmental isolates of Vibrio cholerae non-Ol in Bangladesh. J Med Microbiol1990; 33:107–114
BartowskyE. J.,
AttridgeS. R.,
ThomasC. J.,
MayrhoferG.,
ManningP. A. Role of the P plasmid in attenuation of Vibrio cholerae Ol. Infect Immun1990; 58:3129–3134
TaylorR. K.,
MillerV. L.,
FurlongD. B.,
MekalanosJ. J. Use of PhoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc Natl Acad Sci USA1987; 84:2833–2837
JohnsonJ. A.,
PanigrahiP.,
MorrisJ. G. Non-Ol V. cholera NRT36S produces a polysaccharide capsule that determines colony morphology, serum resistance, and virulence in mice. Infect Immun1992; 60:864–869
JohnsonJ. A.,
SallesC. A.,
PanigrahiP.Vibrio cholerae O139 synonym Bengal is closely related to Vibrio cholerae El Tor but has important differences. Infect Immun1994; 62:2108–2110