1887

Abstract

Surmmary

DNA hybridisation of 309 consecutive clinical isolates with oligonucleotide probes specific for genes encoding Panton-Valentine leucocidin (luk-PV) and y-haemolysin (hlg) revealed that 99% of randomly selected strains carried the hlg locus whereas only 2% harboured the -PV as well as the loci. Only 1% of the strains did not possess either gene. In a clinical prospective study of independent strains, 58 Panton-Valentine leucocidin (PVL)-producing isolates were shown to be responsible for primary skin infections, mainly furuncles (86 %). Phage susceptibility patterns and pulsed field gel electrophoresis (PFGE) profiles of DNA were shown to be polymorphic epidemiological markers of PVL-producing strains. In eight patients with recurrent furuncles, the PVL-producing strains isolated either from furuncles or from the anterior nares were considered to be identical in each based upon phage sensitivity profiles or PFGE patterns.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-42-4-237
1995-04-01
2022-12-08
Loading full text...

Full text loading...

/deliver/fulltext/jmm/42/4/medmicro-42-4-237.html?itemId=/content/journal/jmm/10.1099/00222615-42-4-237&mimeType=html&fmt=ahah

References

  1. Woodin A. M. The staphylococcal leukocidin. In Cohen J. O. (ed) The staphylococci New York: Wiley Interscience; 1972281–289
    [Google Scholar]
  2. Guyonnet F., Plommet M. Hémolysine gamma de Staphylococcus aureus: purification et propriétés. Ann Inst Pasteur 1970; 118:19–33
    [Google Scholar]
  3. Taylor A. G., Bernheimer A. W. Further characterization of staphylococcal gamma-hemolysin. Infect Immun 1974; 10:54–59
    [Google Scholar]
  4. Prevost G., Supersac G., Colin D. A. The new family of leucotoxins from Staphylococcus aureus: structural and biological properties. In Freer . (eds) Bacterial protein toxins. Zentralbl Bakteriol Suppl 24 Gustav Fischer Verlag, Stuttgart, Jena; New York: 1994284–293
    [Google Scholar]
  5. Supersac G., Prevost G., Piemont Y. Sequencing of leucocidin R from Staphylococcus aureus P83 suggests that staphylococcal leucocidins and gamma-hemolysin are members of a single, two-component family of toxin. Infect Immun 1993; 61:580–587
    [Google Scholar]
  6. Gladstone G. P., Van Heyningen W. E. Staphylococcal leucocidins. Br J Exp Pathol 1957; 38:123–137
    [Google Scholar]
  7. Panton P. N., Valentine F. C. O. Staphylococcal toxin. Lancet 1932; 1:506–508
    [Google Scholar]
  8. Finck-Barbangon V., Prevost G., Piemont Y. Improved purification of leukocidin from Staphylococcus aureus and toxin distribution among hospital strains. Res Microbiol 1991; 142:75–85
    [Google Scholar]
  9. Woodin A. M. Purification of the two components of leucocidin from Staphylococcus aureus . Biochem J 1960; 75:158–165
    [Google Scholar]
  10. Soboll H., Ito A., Schaeg W., Blobel H. Leukozidin von Staphylokokken Verscheidener Herkunft. Zentralbl Bakteriol Orig A 1973; 224:184–193
    [Google Scholar]
  11. Kamio Y., Rahman A., Nariya H., Ozawa T., Izaki K. The two staphylococcal bi-component toxins, leukocidin and gamma-hemolysin, share one component in common. FEBS Lett 1993; 321:15–18
    [Google Scholar]
  12. Noda M., Hirayama T., Kato I., Matsuda F. Crystallization and properties of staphylococcal leukocidin. Biochem Biophys Acta 1980; 633:33–44
    [Google Scholar]
  13. Rahman A., Nariya H., Izaki K., Kato I., Kamio Y. Molecular cloning and nucleotide sequence of leukocidin F-component gene (lukF) from methicillin resistant Staphylococcus aureus . Biochem Biophys Res Commun 1992; 184:640–646
    [Google Scholar]
  14. Rahman A., Izaki K., Kamio Y. Gamma-hemolysin genes in the same family with lukF and lukS genes in methicillin resistant Staphylococcus aureus . Biosci Biotech Biochem 1993; 57:1234–1236
    [Google Scholar]
  15. Clyne M., Birbeck T. H., Arbuthnott J. P. Characterization of staphylococcal gamma-lysin. J Gen Microbiol 1992; 138:923–930
    [Google Scholar]
  16. Cooney J., Kienle Z., Foster T. J., O’Toole P. W. The gamma-hemolysin locus of Staphylococcus aureus comprises three linked genes, two of which are identical to the genes for F and S components of leucocidin. Infect Immun 1993; 61:768–771
    [Google Scholar]
  17. Cribier B., Prévost G., Couppié P., Finck-Barbanςon V., Grosshans E., Piémont Y. Staphylococcus aureus leuco-cidin: a new virulence factor in cutaneous infections? An epidemiological and experimental study. Dermatology 1992; 185:175–180
    [Google Scholar]
  18. Ward P. D., Turner W. H. Identification of staphylococcal Panton-Valentine leukocidin as a potent dermonecrotic toxin. Infect Immun 1980; 28:393–397
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T. (eds) Molecular cloning: a laboratory manual 2nd edn Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press; 1989
    [Google Scholar]
  20. Southern E. M. Detection of specific sequence among DNA fragments separated by gel electrophoresis. J Mol Biol 1975; 98:503–517
    [Google Scholar]
  21. Blair J. E., Williams R. E. O. Phage typing of staphylococci. Bull WHO 1961; 24:771–784
    [Google Scholar]
  22. Prevost G., Jaulhac B., Piemont Y. DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates. J Clin Microbiol 1992; 30:967–973
    [Google Scholar]
  23. Hedström S. Å. Recurrent furonculosis. Bacteriological findings and epidemiology in 100 cases. Stand J Infect Dis 1981; 13:115–119
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-42-4-237
Loading
/content/journal/jmm/10.1099/00222615-42-4-237
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error