1887

Abstract

Surmmary

Chemotactic responses of blood neutrophils and monocytes to media conditioned by eight strains of with different virulence characteristics were measured in modified Boyden assay chambers to determine if these characteristics were associated with differences in leucocyte mobility. Responding neutrophils and monocytes were prepared on conventional density gradients, and in three instances, the chemotaxis of eosinophils isolated on metrizamide gradients was also studied. Media conditioned by enteroinvasive and non-enteroinvasive strains were tested as chemo-attractants and compared to the formylated peptide standard attractant. Chemotactic activity of neutrophils was greater than that of monocytes and eosinophils, and migration by all populations was significantly greater to conditioned media than to the control medium. Chemotactic responses to media conditioned by non-enteroinvasive and strains lacking virulence factors was greater than to media conditioned by plasmid- and Sereny-positive enteroinvasive organisms. The results suggest that virulence factors of that determine invasiveness did not augment the chemotactic responses of the leucocyte populations tested , and give no support to the hypothesis that they induce mucosal inflammation by directly increasing chemotaxis .

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/content/journal/jmm/10.1099/00222615-42-3-196
1995-03-01
2022-08-13
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References

  1. Jass J. R. The large intestine. In Morson B. C. (ed) Alimentary tract vol 3 of Symmers W St C (ed) Systemic pathology, 3rd edn Edinburgh: Churchill Livingstone; 1987313–395
    [Google Scholar]
  2. Whitehead R. Mucosal biopsy of the gastrointestinal tract. In Bennington J. L. (ed) Major problems of pathology vol 3 Philadelphia W. B.: Saunders Co; 1985209
    [Google Scholar]
  3. Falk W., Goodwin R. H., Leonard E. J. A 48-well micro-chemo taxis assembly for rapid and accurate measurement of leukocyte migration. J Immunol Methods 1980; 33:239–247
    [Google Scholar]
  4. Zigmond S. H., Hirsch J. G. Leukocyte locomotion and chemo taxis : New methods for evaluation and demonstration of a cell-derived chemotactic factor. J Exp Med 1973; 137:387–410
    [Google Scholar]
  5. Roos D., Loos J. A. Changes in the carbohydrate metabolism of mitogenically stimulated human peripheral lymphocytes. 1. Stimulation by phytohaemagglutinin. Biochim Biophys Acta 1970; 222:565–582
    [Google Scholar]
  6. Beeken W. L., Northwood I., Beliveau C., Baigent G., Gump D. Eosinophils of human colonic mucosa: C3b and Fc gamma receptor expression and phagocytic capabilities. Clin Immunol Immunopathol 1987; 43:289–300
    [Google Scholar]
  7. Montgomery D. C. (ed) Design and analysis of experiments 3rd edn New York: John Wiley and Sons; 1991; 75134–169 439-456
    [Google Scholar]
  8. Miller M. D., Krangel M. S. Biology and biochemistry of the chemokines: a family of chemostatic and inflammatory cytokines. Crit Rev Immunol 1992; 12:17–46
    [Google Scholar]
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