Replication of and in the human monocytic cell line U-937 Free

Abstract

Surmmary

To elucidate whether and infections occur to a significant extent in monocytes-macrophages, the human monocytic cell line, U-937, was infected with L or TW-183. Chlamydial DNA and genus-specific antigens of the lipopolysaccharides (LPS) in epitopes of the chlamydial cell wall were detected from L-inoculated monocytes over a period of 150 days after inoculation and from the TW-183-inoculated cells during a period of 14 days. -infected U-937 cells expressed significantly lower levels of CD4, CD45RA, CD11b and CD33 cells, determined by flow cytometry, than control uninoculated cells on the seventh day after inoculation and they expressed a slightly increased level of CD4 cells and lower levels of CD45RA and CD11b cells on the 14th day after inoculation. -infected U-937 cells expressed significantly lower levels of CD4, CD45RA, CD11b and CD33 cells than controls on the seventh day after inoculation and an increased level of CD4 and a lower level of CD45RA cells on the 14th day after inoculation. Unlike infection with L strain, chronic persistent infection with appears not to occur in monocytes-macrophages.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-42-3-191
1995-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/42/3/medmicro-42-3-191.html?itemId=/content/journal/jmm/10.1099/00222615-42-3-191&mimeType=html&fmt=ahah

References

  1. Numazaki K., Wainberg M. A., McDonald J. Chlamydia tracho matis infections in infants. Can Med Assoc J 1989; 140:615–622
    [Google Scholar]
  2. Grayston J. T. Infections caused by Chlamydia pneumoniae strain TWAR. Clin Infect Dis 1992; 15:757–763
    [Google Scholar]
  3. Numazaki K., Nagata N., Sato T., Chiba S. Effect of glycyrrhizin, cyclosporin A, and tumor necrosis factor α on infection of U-937 and MRC-5 cells by human cytomegalovirus. J Leukoc Biol 1994; 55:24–28
    [Google Scholar]
  4. Numazaki K., Chiba S., Nakata S., Yamanaka T., Nakao T. Prevalence of antibodies to Chlamydia trachomatis in Japanese persons determined by microimmunofluorescence using reticulate bodies as single antigen. Pediatr Infect Dis 1984; 3:105–109
    [Google Scholar]
  5. Rodriguez P., de Barbeyrac B., Persson K., Dutilh B., Bebear C. Evaluation of molecular typing for epidemiological study of Chlamydia trachomatis genital infections. J Clin Microbiol 1993; 31:2238–2240
    [Google Scholar]
  6. Frost E. H., Deslandes S., Veilleux S., Bourgaux-Ramoisy D. Typing Chlamydia trachomatis by detection of restriction fragment length polymorphism in the gene encoding the major outer membrane protein. J Infect Dis 1991; 163:1103–1107
    [Google Scholar]
  7. Campbell L. A., Perez Melgosa M., Hamilton D. J., Kuo C.-C., Grayston J. T. Detection of Chlamydia pneumoniae by polymerase chain reaction. J Clin Microbiol 1992; 30:434–439
    [Google Scholar]
  8. Numazaki K., Chiba S., Yamanaka T., Umetsu M., Nakao T. Pneumonia due to Chlamydia trachomatis in Japanese infants. Tohoku J Exp Med 1984; 143:413–420
    [Google Scholar]
  9. Numazaki K., Chiba S. Diagnostic value of rapid detection of Chlamydia trachomatis by using amplified enzyme immunoassay in infants with respiratory infections. Diagn Microbiol Infect Dis 1993; 17:233–234
    [Google Scholar]
  10. Numazaki K., Chiba S., Umetsu M. Detection of IgM antibodies to Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci from Japanese infants and children with pneumonia. In Vivo 1992; 6:601–604
    [Google Scholar]
  11. Rothermel C. D., Schachter J., Lavrich P., Lipsitz E. C., Francus T. Chlamydia trachomatis-induced production of interleukin-1 by human monocytes. Infect Immun 1989; 57:2705–2711
    [Google Scholar]
  12. Nakajo M. N., Roblin P. M., Hammerschlag M. R., Smith P., Nowakowski M. Chlamydicidal activity of human alveolar macrophages. Infect Immun 1990; 58:3640–3644
    [Google Scholar]
  13. Manor E., Sarov I. Fate of Chlamydia trachomatis in human monocytes and monocytes-derived macrophages. Infect Immun 1986; 54:90–95
    [Google Scholar]
  14. Yong E. C., Chi E. Y., Kuo C.-C. Differential antimicrobial activity of human mononuclear phagocytes against the human biovars of Chlamydia trachomatis. J Immunol 1987; 139:1297–1302
    [Google Scholar]
  15. Pal S., Fielder T. J., Peterson E. M., de la Maza L. M. Analysis of the immune response in mice following intrauterine infection with the Chlamydia trachomatis mouse pneumonitis biovar. Infect Immun 1993; 61:772–776
    [Google Scholar]
  16. Young E., Taylor H. R. Immune mechanisms in chlamydial eye infection: cellular immune responses in chronic and acute diseases. J Infect Dis 1984; 150:745–751
    [Google Scholar]
  17. Mabey D. C. W., Holland M. J., Viswalingam N. D. Lymphocyte proliferative responses to chlamydial antigens in human chlamydial eye infections. Clin Exp Immunol 1991; 86:37–42
    [Google Scholar]
  18. Boulerice F., Bour S., Geleziunas R., Lvovich A., Wainberg M. A. High frequency of isolation of defective human immunodeficiency virus type 1 and heterogeneity of viral gene expression in clones of infected U-937 cells. J Virol 1990; 64:1745–1755
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-42-3-191
Loading
/content/journal/jmm/10.1099/00222615-42-3-191
Loading

Data & Media loading...

Most cited Most Cited RSS feed