1887

Abstract

Surmmary

The production of a family of haemolysins—thermostable direct haemolysin (Vp-TDH), Vp-TDH-related haemolysin (Vp-TRH) and Vp-TDH/I—has been reported in clinical isolates of . This paper describes a fourth type of haemolysin—Vp-TDH/II—produced by a Kanagawa phenomenon-negative clinical isolate of (O13: K, untypable). Vp-TDH/II was purified by ammonium sulphate precipitation and successive filtrations on DEAE-cellulose, hydroxyapatite, Sepharose 4B and Mono Q columns. Vp-TDH/II was biophysicochemically and immunologically similar to, but not identical to Vp-TDH, Vp-TRH and Vp-TDH/I. Vp-TDH/II stimulated vascular permeability in rabbit skin and was lethal to mice. Purified Vp-TDH/II and viable cells of the Vp-TDH/II-producing strain both induced fluid accumulation in ligated rabbit intestine. The plasmid-determined structural gene for Vp-TDH/II was cloned and the nucleotide sequence determined. The deduced amino acid sequence of Vp-TDH/II differed from those of Vp-TDH, Vp-TRH and Vp-TDH/I.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-42-2-83
1995-02-01
2024-05-30
Loading full text...

Full text loading...

/deliver/fulltext/jmm/42/2/medmicro-42-2-83.html?itemId=/content/journal/jmm/10.1099/00222615-42-2-83&mimeType=html&fmt=ahah

References

  1. Fujino T., Okuno Y., Nakada D. On the bacteriological examination of shirasu food poisoning. Med J Osaka Univ 1953; 4:299–304
    [Google Scholar]
  2. Miwatani T., Takeda Y. Vibrio parahaemolyticus—a causative bacterium of food poisoning. Tokyo: Saikon Publishers; 19761–109
    [Google Scholar]
  3. Joseph S. W., Colwell R. R., Kaper J. B. Vibrio parahaemolyticus and related halophilic Vibrios. Crit Rev Microbiol 1982; 10:77–124
    [Google Scholar]
  4. Janda J. M., Powers C., Bryant R. G., Abbott S. L. Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp. Clin Microbiol Rev 1988; 1:245–267
    [Google Scholar]
  5. Miyamoto Y., Kato T., Obara Y. In vitro hemolytic characteristic of Vibrio parahaemolyticus; its close correlation with human pathogenicity. J Bacteriol 1969; 100:1147–1149
    [Google Scholar]
  6. Honda T., Taga S., Takeda Y. Identification of lethal toxin with the thermostable direct haemolysin produced by Vibrio parahaemolyticus, and some physicochemical properties of the purified toxin. Infect Immun 1976; 13:133–139
    [Google Scholar]
  7. Miyamoto Y., Obara Y., Nikkawa T. Simplified purification and biophysicochemical characteristics of Kanagawa phenomenon-associated haemolysin of Vibrio parahaemolyticus. Infect Immun 1980; 23:567–576
    [Google Scholar]
  8. Sakazaki R., Tamura K., Kato T. Studies on the entero-pathogenic, facultatively halophilic bacterium, Vibrio parahaemolyticus. III. Enteropathogenicity. Jpn J Med Sci Biol 1968; 21:325–331
    [Google Scholar]
  9. Honda S., Goto I., Minematsu I. Gastroenteritis due to Kanagawa negative Vibrio parahaemolyticus. Lancet 1987; 1:331–332
    [Google Scholar]
  10. Honda T., Ni Y., Miwatani T. Purification and characterization of a haemolysin produced by a clinical isolate of Kanagawa phenomenon negative Vibrio parahaemolyticus and related to the thermostable direct haemolysin. Infect Immun 1988; 56:961–965
    [Google Scholar]
  11. Honda T., Ni Y., Miwatani T. Purification of a TDH-related hemolysin produced by a Kanagawa phenomenon-negative clinical isolate of Vibrio parahaemolyticus O6:K46. FEMS Microbiol Lett 1989; 48:241–245
    [Google Scholar]
  12. Honda T., Ni Y., Yoh M., Miwatani T. Production of monoclonal antibodies against thermostable direct haemolysin of Vibrio parahaemolyticus and application of the antibodies for enzyme-linked immunosorbent assay. Med Microbiol Immunol 1989; 178:245–253
    [Google Scholar]
  13. Nishibuchi M., Taniguchi T., Misawa T., Khaeomance-Iaun V., Honda T., Miwatani T. Cloning and nucleotide sequence of the gene (tdh) encoding the hemolysin related to the thermostable direct haemolysin of Vibrio parahaemolyticus. Infect Immun 1989; 57:2691–2697
    [Google Scholar]
  14. Honda T., Abad-Lapuebla M. A., Yamamoto K., Miwatani T. Characterization of a new thermostable direct haemolysin produced by a Kanagawa phenomenon-negative clinical isolate of Vibrio parahaemolyticus. J Gen Microbiol 1991; 137:253–259
    [Google Scholar]
  15. Honda T., Chearskul S., Takeda Y., Miwatani T. Immunological methods for detection of Kanagawa phenomenon of Vibrio parahaemolyticus. J Clin Microbiol 1980; 11:600–603
    [Google Scholar]
  16. Honda T., Honda S., Ni Y. X., Miwatani T. Development and application of enzyme-linked immunosorbent assay using monoclonal antibody against a hemolysin (Vp-TRH) of Vibrio parahaemolyticus—evidence that Vp-TRH-produc-ing Kanagawa phenomenon-negative Vibrio parahaemolyticus is a human pathogen. Kasenshogaku Zasshi (J Jpn Assoc Infect Dis) 1990; 64:767–773
    [Google Scholar]
  17. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13 mpl8 and pUC19 vectors. Gene 1985; 33:103–119
    [Google Scholar]
  18. Yamamoto K., Takeda Y., Miwatani T., Craig J. P. Purification and some properties of a non-O1 Vibrio cholerae entero-toxin that is identical to cholera enterotoxin. Infect Immun 1983; 39:1128–1135
    [Google Scholar]
  19. Honda T., Goshima K., Takeda Y. Demonstration of the cardiotoxicity of the thermostable direct haemolysin (lethal toxin) produced by Vibrio parahaemolyticus. Infect Immun 1976; 13:163–171
    [Google Scholar]
  20. Twedt R. M., Peeler J. T., Spaulding P. L. Effective ileal loop dose of Kanagawa-positive Vibrio parahaemolyticus. Appl Environ Microbiol 1980; 40:1012–1016
    [Google Scholar]
  21. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 1979; 7:1513–1523
    [Google Scholar]
  22. Silhavy T. J., Berman M. L., Enquist L. W. Experiments with gene fusions. Cold Spring Harbor: NY, Cold Spring Harbor Laboratory; 1984
    [Google Scholar]
  23. Maniatis T., Fritsch E. F., Sambrook J. Molecular cloning: a laboratory manual. Cold Spring Harbor: NY, Cold Spring Harbor Laboratory; 1982
    [Google Scholar]
  24. Feinberg A. P., Vogelstein B. A. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 1983; 132:6–13
    [Google Scholar]
  25. Southern E. Gel electrophoresis of restriction fragments. Methods Enzymol 1979; 68:152–176
    [Google Scholar]
  26. Sanger F., Nicklen S., Couson A. R. DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 1977; 74:5463–5467
    [Google Scholar]
  27. Honda T., Ni Y. X., Hata A. Properties of a haemolysin related to the thermostable direct haemolysin produced by a Kanagawa phenomenon negative, clinical isolate of V. parahaemolyticus. Can J Microbiol 1990; 36:395–399
    [Google Scholar]
  28. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 1977; 2:95–113
    [Google Scholar]
  29. Shine J., Dalgarno L. The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA: complementary to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci USA 1974; 71:1342–1346
    [Google Scholar]
  30. Rosenberg M., Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 1979; 13:319–353
    [Google Scholar]
  31. Iida T., Yamamoto K. Cloning and expression of two genes encoding highly homologous hemolysins from a Kanagawa phenomenon-positive strain Vibrio parahaemolyticus T4750 strain. Gene 1990; 93:9–15
    [Google Scholar]
  32. Nishibuchi M., Kaper J. B. Duplication and variation of the thermostable direct haemolysin (tdh) gene in Vibrio parahaemolyticus. Mol Microbiol 1990; 4:87–99
    [Google Scholar]
  33. Honda T., Nishibuchi M., Miwatani T., Kaper J. B. Demon stration of plasmid-borne gene encoding a thermostable direct haemolysin in Vibrio cholerae non-O1 strains. Appl Environ Microbiol 1986; 52:1218–1220
    [Google Scholar]
  34. Terai A., Shirai H., Yoshida O., Takeda Y., Nishibuchi M. Nucleotide sequence of the thermostable direct haemolysin gene (tdh gene) of Vibrio mimicus and its evolutionary relationship with the tdh genes of Vibrio parahaemolyticus. FEMS Microbiol Lett 1990; 59:319–323
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-42-2-83
Loading
/content/journal/jmm/10.1099/00222615-42-2-83
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error