1887

Abstract

Surmmary

The antigenic profiles of trophozoites and cysts were compared by immunoblotting with hyperimmune rat sera against cyst and trophozoite antigens. Strong bands corresponding to proteins of 50–60 kDa and 104 kDa were demonstrated in cyst and trophozoite antigens by all antisera. Additional prominent proteins of 81 and 63 kDa and less prominent proteins of 88, 73, 69 and 37 kDa were found only in trophozoite antigen. The latter proteins were recognised by anti-trophozoite and anti-cyst antisera but the 81- and 63-kDa proteins were associated specifically with trophozoites. With cyst-rich antigen, antibodies to the 50–60-kDa protein were detected in only two of 14 sera from pneumonia (PCP)-positive rats. With trophozoite-rich antigen, 11 of 24 rats with PCP and one of 18 PCP-negative animals had antibodies to both the 50–60 kDa and 104-kDa antigens. Antibodies to the 81- or 63-kDa antigens were demonstrated in 15 of 24 PCP-positive animals and none of the PCP-negative animals. The use of trophozoites rather than cysts increased the sensitivity of immunoblotting. As trophozoites predominate in PCP, antibody to trophozoite-specific antigens rather than common cyst and trophozoite antigens is likely to be a more useful marker of current infection.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-42-2-120
1995-02-01
2022-09-30
Loading full text...

Full text loading...

/deliver/fulltext/jmm/42/2/medmicro-42-2-120.html?itemId=/content/journal/jmm/10.1099/00222615-42-2-120&mimeType=html&fmt=ahah

References

  1. Chatterton J. M. W., Joss A. W. L., Davidson M. M., Ho-Yen D. O. Why have Pneumocystis carinii trophozoites been ignored?. J Clin Pathol 1990; 43:265–268
    [Google Scholar]
  2. Masur H., Jones T. C. The interaction in vitro of Pneumocystis carinii with macrophages and L-cells. J Exp Med 1978; 147:157–170
    [Google Scholar]
  3. Walzer P. D., Rutledge M. E., Yoneda K., Stahr B. J. Pneumocystis carinii: new separation me thod from lung tissue. Exp Parasitol 1979; 47:356–368
    [Google Scholar]
  4. Ishimaru T., Shimono N., Sawae Y., Niho Y. Purification of Pneumocystis carinii trophozoites and identification of their circulating antigens. J Clin Microbiol 1992; 30:3263–3267
    [Google Scholar]
  5. Gosey L. L., Howard R. M., Witebsky F. G. Advantages of a modified toluidine blue O stain and bronchoalveolar lavage for the diagnosis of Pneumocystis carinii pneumonia. J Clin Microbiol 1985; 22:803–807
    [Google Scholar]
  6. Chatterton J. M. W., Joss A. W. L., Williams H., Ho-Yen D. O. Pneumocystis carinii antibody testing. J Clin Pathol 1989; 42:865–868
    [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685
    [Google Scholar]
  8. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76:4350–4354
    [Google Scholar]
  9. Gradus M. S. Ivey MH. An improved method of isolating Pneumocystis carinii from infected rat lungs. J Parasitol 1986; 72:690–698
    [Google Scholar]
  10. Read J. A., Burns S. M. A simple method of separation of Pneumocystis carinii from rat lung. Int J Exp Pathol 1991; 72:673–677
    [Google Scholar]
  11. Watanabe J., Tanabe K., Shimada K. Separation of trophozoites of Pneumocystis carinii from lung of the inoculated nude rat. Jpn J Exp Med 1987; 57:295–297
    [Google Scholar]
  12. McNabb S. J. N., Graves D. C., Kosanke S. D., Moyer M. J., Ivey M. H. Pneumocystis carinii antigen detection in rat serum and lung lavage. J Clin Microbiol 1988; 26:1763–1771
    [Google Scholar]
  13. Walzer P. D., Linke M. J. A comparison of the antigenic characteristics of rat and human Pneumocystis carinii by immunoblotting. J Immunol 1987; 138:2257–2265
    [Google Scholar]
  14. Graves D. C., McNabb S. J. N., Worley M. A., Downs T. D., Ivey M. H. Analyses of rat Pneumocystis carinii antigens recognized by human and rat antibodies by using Western immunoblotting. Infect Immun 1986; 54:96–103
    [Google Scholar]
  15. Walzer P. D., Stanforth D., Linke M. J., Cushion M. T. Pneumocystis carinii: immunoblotting and immunofluorescent analyses of serum antibodies during experimental rat infection and recovery. Exp Parasitol 1987; 63:319–328
    [Google Scholar]
  16. Malin A. S., Miller R. F. Pneumocystis carinii pneumonia; pres entation and diagnosis. Rev Med Microbiol 1992; 3:80–87
    [Google Scholar]
  17. Ho-Yen D. O., Chatterton J. M. W., Joss A. W. L. Pneumocystis carinii pneumonia. BMJ 1988; 297:855
    [Google Scholar]
  18. Chatterton J. M. W., Ho-Yen D. O. Laboratory investigation of Pneumocystis carinii pneumonia. Genitourin Med 1992; 68:336–341
    [Google Scholar]
  19. Contini C., Romani R., Vullo V., Delia S., Sorice F. Does laboratory recovery of Pneumocystis carinii always mean clinically significant disease?. AIDS 1992; 6:1558–1559
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-42-2-120
Loading
/content/journal/jmm/10.1099/00222615-42-2-120
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error