1887

Abstract

Surmmary

Several inbred strains of mice in closed breeding colonies were found to have spiral-shaped bacteria associated with active, chronic hepatitis. A new species of , was isolated from the infected livers of some strains of mice. Other strains of mice were colonised with in the caecum and colon, but not the liver. Filter-sterilised supernatant fluid from five strains of was tested in a mouse liver cell line (ATCC no. CCL 9.1) for cytotoxic activity. All strains produced a toxic factor causing morphological changes in the cells at dilutions up to 1 in 1000. Toxicity was observed after exposure to the supernatant fluid for 48–72 h. Other spp. that also produced the cytopathic effect (CPE) in the liver cell line were and one strain of and did not cause CPE in the liver cells. The soluble factor was stable at 4°C for up to 3 months. It was also stable at 56°C for 30 min, but was inactivated by boiling for 15 min. It was inactivated by incubation with trypsin. A partially purified preparation of the cytotoxin had a mol. wt of . 100000 and did not have urease activity. The cytotoxin produced by did not cause vacuole formation in HeLa cells.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-42-1-48
1995-01-01
2024-12-04
Loading full text...

Full text loading...

/deliver/fulltext/jmm/42/1/medmicro-42-1-48.html?itemId=/content/journal/jmm/10.1099/00222615-42-1-48&mimeType=html&fmt=ahah

References

  1. Graham D. Y. Campylobacter pylori and peptic ulcer disease. Gastroenterology 1989; 96:615–625
    [Google Scholar]
  2. Lee A., Fox J., Hazell S. Pathogenicity of Helicobacter pylori: a perspective. Infect Immun 1993; 61:1601–1610
    [Google Scholar]
  3. Marshall B. J., Warren J. R. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1984; 1:1311–1314
    [Google Scholar]
  4. Fox J. G., Correa P., Taylor N. S. Campylobacter pylori-associated gastritis and immune response in a population at high risk of gastric carcinoma. Am J Gastroenterol 1989; 89:775–778
    [Google Scholar]
  5. Nomura A., Stemmermann G. N., Chyou P. H., Kato I., Perez-Perez G. I., Blaser M. J. Helicobacter pylori infection and gastric carcinoma among Japanese Americans in Hawaii. N Engl J Med 1991; 325:1132–1136
    [Google Scholar]
  6. Parsonnet J., Friedman G. D., Vandersteen D. P. Helicobacterpylori infection and the risk of gastric carcinoma. N Engl J Med 1991; 325:1127–1131
    [Google Scholar]
  7. Wotherspoon A. C., Doglioni C., Dis T. C. Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue type after eradication of Helicobacter pylori. Lancet 1993; 342:575–577
    [Google Scholar]
  8. Fox J. G., Blanco M., Murphy J. C. Local and systemic immune responses in murine Helicobacter felis active chronic gastritis. Infect Immun 1993; 61:2309–2315
    [Google Scholar]
  9. Fox J. G., Correa P., Taylor N. S. Helicobacter mustellae-associated gastritis in ferrets. An animal model of Helicobacter pylori gastritis in human. Gastroenterology 1990; 99:352–361
    [Google Scholar]
  10. Fox J. G., Lee A. Gastric Helicobacter infection in animals: natural and experimental infections. In Goodwin C. S., Worsley B. W. (eds) Biology and clinical practice Boca Raton: CRC Press; 1993407–430
    [Google Scholar]
  11. Lee A., Phillips M. W., O’Rourke J. L. Helicobacter muridarum sp. nov., a microaerophilic helical bacterium with a novel ultrastructure isolated from the intestinal mucosa of rodents. Int J Syst Bacteriol 1992; 42:27–36
    [Google Scholar]
  12. Queiruz D. M. M., Contigli C., Coimbra R. S. Spiral bacterium associated with gastric, ileal, and cecal mucosa of mice. Lab Animal 1992; 26:288–294
    [Google Scholar]
  13. Schauer D. B., Ghori N., Falkows S. Isolation of “Flexispirarappini” from laboratory mice. J Clin Microbiol 1993; 31:2709–2714
    [Google Scholar]
  14. Ward J. M., Fox J. G., Anver M. R. Chronic active hepatitis and associated liver tumors in mice caused by a persistent bacterial infection with a novel Helicobacter species. (in press)
  15. Ward J. M., Anver M. R., Hines D. C. Chronic active hepatitis of unknown origin in mice from a large research facility. Vet Pathol 1993; 30:4769
    [Google Scholar]
  16. Fox J. G., Tully J. G., Dewhirst F. E. Helicobacter hepaticus sp. nov., a microaerophilic bacteria isolated from liver and intestinal mucosa of mice. J Clin Microbiol (in press)
    [Google Scholar]
  17. Figura N., Guglielmetti N., Rossolini A. Cytotoxin production by Campylobacter pylori strains isolated from patients with peptic ulcers and from patients with chronic gastritis only. J Clin Microbiol 1989; 27:225–226
    [Google Scholar]
  18. Leunk R. D., Johnson P. T., David B. C., Kraft W. G., Morgan D. R. Cytotoxic activity in broth-culture filtrates of Campylobacter pylori. J Med Microbiol 1988; 26:93–99
    [Google Scholar]
  19. Cover T. L., Blaser M. J. Purification and characterization of the vacuolating toxin from Helicobacter pylori. J Biol Chem 1992; 267:10570–10575
    [Google Scholar]
  20. Figura N., Bugnoli M., Guglielmetti P., Musmanno R. A., Russi M., Quaranta S. Antibodies to vacuolating toxin of Helicobacter pylori in dyspeptic patients. Rev Esp Enferm Dig 1991; 78: Suppl 17
    [Google Scholar]
  21. Crabtree J. E., Taylor J. D., Wyatt J. I. Mucosal IgA recognition of Helicobacter pylori 120-kDa protein, peptic ulceration, and gastric pathology. Lancet 1991; 338:332–335
    [Google Scholar]
  22. Tummuri M. R. K., Cover T. L., Blaser M. J. Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori: evidence of linkage to cytotoxin production. Infect Immun 1993; 61:1799–1809
    [Google Scholar]
  23. Taylor N. S., Bartlett J. G. Partial purification and characterization of a cytotoxin from Clostridium difficile. Rev Infect Dis 1979; 1:379–385
    [Google Scholar]
  24. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J 1964; 91:222–233
    [Google Scholar]
  25. Dunn B. E., Campbell G. P., Perez-Perez G. I., Blaser M. J. Purification and characterization of urease from Helicobacter pylori. J Biol Chem 1990; 265:9464–9469
    [Google Scholar]
  26. Kita E., Katsui N., Nishi K., Emoto M., Yanagase Y., Kashiba S. Hepatic lesions in experimental Campylobacter jejuni infection of mice. J Gen Microbiol 1986; 132:3095–3103
    [Google Scholar]
  27. Kita E., Okie D., Hamuro A. Hepatotoxic activity of Campylobacter jejuni. J Med Microbiol 1990; 33:171–182
    [Google Scholar]
  28. Kita E., Nishikawa F., Kamikaidou N., Nakano A., Katsui N., Kashiba S. Mononuclear cell response in the liver of mice infected with hepatotoxogenic Campylobacter jejuni. J Med Microbiol 1992; 37:326–331
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-42-1-48
Loading
/content/journal/jmm/10.1099/00222615-42-1-48
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error