%0 Journal Article %A Giugliano, L. G. %A Ribeiro, S. T. G. %A Vainstein, M. H. %A Ulhoa, C. J. %T Free secretory component and lactoferrin of human milk inhibit the adhesion of enterotoxigenic Escherichia coli %D 1995 %J Journal of Medical Microbiology, %V 42 %N 1 %P 3-9 %@ 1473-5644 %R https://doi.org/10.1099/00222615-42-1-3 %I Microbiology Society, %X Surmmary The non-immunoglobulin component of human milk responsible for the inhibition of Escherichia coli cell adhesion (haemagglutination) mediated by colonisation factor antigen 1 (CFA1) was determined by chromatographic fractionation of human whey proteins with Sephadex G-200, DEAE cellulose and heparin-sepharose. Pure free secretory component (fSC) and pure lactoferrin (Lf) were isolated and both compounds inhibited the haemagglutination induced by E. coli CFA1+. The lowest concentrations of purified fSC and Lf able to inhibit the haemagglutination induced by E. coli strain TR50/3 CFA1+ were 0.06 mg/ml and 0.1 mg/ml respectively. Commercially available lactoferrin from human milk and transferrin from human serum, which has a great structural analogy to lactoferrin, also inhibited the haemagglutination. The lowest concentrations of the commercial lactoferrin and transferrin able to inhibit the haemagglutination induced by E. coli TR50/3 CFA1+ were 0.03 mg/ml and 0.4mg/ml, respectively. These results indicate that fSC and Lf may be important non-specific defence factors against enterotoxigenic E. coli infections. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-42-1-3