1887

Abstract

Summary

An antibiogram-resistogram (AR) typing scheme that can simply and rapidly differentiate methicillin-resistant (MRSA) isolates has been devised. Susceptibility to antibiotics and chemicals was determined by disk diffusion testing. Three disk diffusion methods and three control strains were evaluated. A modified Stokes’ technique in which ATCC 25923 replaced NCTC 6571 as the control organism was chosen. Susceptibility patterns against 18 antibiotics and four chemicals were used to determine AR types. AR subtypes were determined with reference to knowledge of the local MRSA population so that plasmid loss would not result in misclassification. AR typing was compared with phage typing and plasmid profiling and found to be more discriminatory than either of these typing methods. Representative isolates of the most frequently occurring AR patterns were further characterised by investigating enterotoxin production, MICs of gentamicin and amikacin, and restriction endonuclease analysis of plasmid DNA, to determine whether apparently different strains could have the same AR pattern and to devise confirmatory tests for any such similar patterns. One pattern was shared by two strains but isolates could be differentiated by susceptibility to minocycline. This typing scheme can be used in the diagnostic laboratory and results may be obtained within 24 h.

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1994-12-01
2022-08-15
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References

  1. Duckworth GJ. Methicillin-resistant Staphylococcus aureus. In Reeves DS, Geddes AM. (eds) Recent advances in infection no. 3 Edinburgh: Churchill Livingstone; 1989189–207
    [Google Scholar]
  2. Cookson BD. Epidemiology and control of nosocomial methicillin-resistant Staphylococcus aureus. Curr Opin Infect Dis 1991; 4:530–535
    [Google Scholar]
  3. Cafĩerkey MT. Therapy for staphylococcal infection. Curr Opin Infect Dis 1991; 4:757–763
    [Google Scholar]
  4. Lyon BR, Skurray R. Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev 1987; 51:88–134
    [Google Scholar]
  5. Cafferkey MT, Coleman D, McGrath B. et al. Methicillin-resistant Staphylococcus aureus in Dublin 1974–84. Lancet 1985; 2:705–708
    [Google Scholar]
  6. Morgan MG, Harte-Barry MJ. Methicillin-resistant Staphylococcus aureus: a ten-year survey in a Dublin hospital. J Hosp Infect 1989; 14:357–362
    [Google Scholar]
  7. Rossney AS, Pomeroy HM, Keane CT. Staphylococcus aureus phage typing, antimicrobial susceptibility patterns and patient data correlated using a personal computer: advantages for monitoring the epidemiology of MRSA. J Hosp Infect 1994; 26:219–234
    [Google Scholar]
  8. Working Party Report Revised guidelines for the control of epidemic methicillin-resistant Staphylococcus aureus. J Hosp Infect 1990; 16:351–377
    [Google Scholar]
  9. Mulligan ME, Arbeit RD. Epidemiologic and clinical utility of typing systems for differentiating among strains of methicillin-resistant Staphylococcus aureus. Infect Control Hosp Epidemiol 1991; 12:20–28
    [Google Scholar]
  10. Coia JE, Thomson-Carter F, Baird D, Platt DJ. Characterisation of methicillin-resistant Staphylococcus aureus by biotyping, immunoblotting and restriction enzyme fragmentation patterns. J Med Microbiol 1990; 31:125–132
    [Google Scholar]
  11. Van Belkum A, Bax R, Peerbooms P, Goessens WHF, Van Leeuwen N, Quint WGV. Comparison of phage typing and DNA fingerprinting by polymerase chain reaction for discrimination of methicillin-resistant Staphylococcus aureus strains. J Clin Microbiol 1993; 31:798–803
    [Google Scholar]
  12. Saulnier P, Bourneix C, Prevost G, Andremont A. Random amplified polymorphic DNA assay is less discriminant than pulsed-field gel electrophoresis for typing strains of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 1993; 31:982–985
    [Google Scholar]
  13. Pfaller MA. Typing methods for epidemiologic investigation. In Balows A, Hausler WJ, Herrmann KL, Isenberg HD, Shadomy HJ. (eds) Manual of clinical microbiology, 5th edn.. Washington DC: American Society for Microbiology; 1991171–182
    [Google Scholar]
  14. Farmer JJ. Conventional typing methods. J Hosp Infect 1988; 11: Suppl A 309–314
    [Google Scholar]
  15. Gillespie MT, Lyon BR, Skurray RA. Typing of methicillin-resistant Staphylococcus aureus by antibiotic resistance phenotypes. J Med Microbiol 1990; 31:57–64
    [Google Scholar]
  16. Ericsson HM, Sherris JC. Antibiotic sensitivity testing. Report of an international collaborative study. Acta Pathol Microbiol Scand[B] 1971 Suppl 217:1–90
    [Google Scholar]
  17. European Committee for Clinical Laboratory Standards Guidelines for antimicrobial susceptibility testing by diffusion methods 1988 Number 1
    [Google Scholar]
  18. National Committee for Clinical Laboratory Standards Performance standards for antimicrobial susceptibility tests, 4th edn.. Tentative Standard. NCCLS Document (1989) M2 T4, Vol 8, No 7
    [Google Scholar]
  19. Barry AL. Standardization of antimicrobial susceptibility testing. In Clinics in laboratory medicine antimicrobial susceptibility testing vol. 9, no. 2 Philadelphia: Saunders; 1989203–220
    [Google Scholar]
  20. Working Party Report of the British Society for Antimicrobial Chemotherapy on Antibiotic Sensitivity Testing A guide to sensitivity testing. J Antimicrob Chemother 1991; 27: Suppl D 1–50
    [Google Scholar]
  21. Archer GL, Mayhall CG. Comparison of epidemiological markers used in the investigation of an outbreak of methicillin-resistant Staphylococcus aureus infections. J Clin Microbiol 1983; 18:395–399
    [Google Scholar]
  22. Bigelow N, Ng L-K, Robson HG, Dillon JR. Strategies for molecular characterisation of methicillin- and gentamicin-resistant Staphylococcus aureus in a Canadian nosocomial outbreak. J Med Microbiol 1989; 30:51–58
    [Google Scholar]
  23. Coia JE, Noor-Hussain I, Platt DJ. Plasmid profiles and restriction enzyme fragmentation patterns of plasmids of methicillin-sensitive and methicillin-resistant isolates of Staphylococcus aureus from hospital and the community. J Med Microbiol 1988; 27:271–276
    [Google Scholar]
  24. Melo Cristino JAG, Torres Pereira A. Plasmid analysis of 219 methicillin-resistant Staphylococcus aureus with uncommon profiles isolated in Lisbon. J Hosp Infect 1989; 13:133–141
    [Google Scholar]
  25. Coleman DC, Pomeroy H, Estridge JK. et al. Susceptibility to antimicrobial agents and analysis of plasmids in gentamicin- and methicillin-resistant Staphylococcus aureus from Dublin hospitals. J Med Microbiol 1985; 20:157–167
    [Google Scholar]
  26. Lyon BR, May JW, Skurray RA. Analysis of plasmids in nosocomial strains of multiple-antibiotic-resistant Staphylococcus aureus. Antimicrob Agents Chemother 1983; 23:817–826
    [Google Scholar]
  27. Crossley K, Loesch D, Landesman B, Mead K, Chern M, Strate R. An outbreak of infections caused by strains of Staphylococcus aureus resistant to methicillin and aminoglycosides. 1. Clinical studies. J Infect Dis 1979; 139:273–279
    [Google Scholar]
  28. Townsend DE, Grubb WB, Ashdown N. Gentamicin resistance in methicillin-resistant Staphylococcus aureus. Pathology 1983; 15:169–174
    [Google Scholar]
  29. Cafferkey MT, Hone R, Falkiner FR, Keane CT, Pomeroy H. Gentamicin and methicillin resistant Staphylococcus aureus in Dublin hospitals: clinical and laboratory studies. J Med Microbiol 1983; 16:117–127
    [Google Scholar]
  30. Stokes EJ, Ridgway GL. Clinical bacteriology, 5th edn.. London: Edward Arnold; 1980219–222
    [Google Scholar]
  31. Brown D, Blowers R. Disc methods of sensitivity testing and other semi-quantitative methods. In Reeves DS, Phillips I, Williams JD, Wise R. (eds) Laboratory methods in antimicrobial chemotherapy Edinburgh: Churchill Livingstone; 19788–30
    [Google Scholar]
  32. Rossney AS, English LF, Keane CT. Coagulase testing compared with commercial kits for routinely identifying Staphylococcus aureus. J Clin Pathol 1990; 43:246–252
    [Google Scholar]
  33. Lachica RVF, Genigeoris C, Hoeprich PD. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl Microbiol 1971; 21:585–587
    [Google Scholar]
  34. Annear DI. The effect of temperature on resistance of Staphylococcus aureus to methicillin and some other antibiotics. Med J Aust 1968; 1:444–446
    [Google Scholar]
  35. Hewitt JH, Coe AW, Parker MT. The detection of methicillin resistance in Staphylococcus aureus. J Med Microbiol 1969; 2:443–456
    [Google Scholar]
  36. Anon. Antibiotic sensitivity tests: control strains. In Catalogues of the National Collection of Type Cultures London: Public Health Laboratory Service; 1989127
    [Google Scholar]
  37. Brown DFJ, Warner M, Bradley J, Taylor CED. The quality of antimicrobial susceptibility testing disks. J Antimicrob Chemother 1982; 10:373–382
    [Google Scholar]
  38. Blair JE, Williams REO. Phage typing of staphylococci. Bull World Health Organ 1961; 24:771–784
    [Google Scholar]
  39. Blumberg HM, Rimland D, Kiehlbauch JA, Terry PM, Wachsmuth IK. Epidemiologic typing of Staphylococcus aureus by DNA restriction fragment length polymorphisms of rRNA genes: eludication of the clonal nature of a group of bacteriophage-nontypable, ciprofloxacin-resistant methi-cillin-susceptible Staphylococcus aureus isolates. J Clin Microbiol 1992; 30:362–369
    [Google Scholar]
  40. Rossney AS, Coleman DC, Keane CT. Evaluation of an antibiogram-resistogram typing scheme for methicillin-resistant Staphylococcus aureus. J Med Microbiol 1994; 41:441–447
    [Google Scholar]
  41. Aber RC, Mackel DC. Epidemiologic typing of nosocomial microorganisms. Am J Med 1981; 70:899–905
    [Google Scholar]
  42. Falkiner FR. Epidemiological typing: a user’s view. J Hosp Infect 1988; 11:303–309
    [Google Scholar]
  43. Cookson B, Talsania H, Naidoo J, Phillips I. Strategies for typing and properties of epidemic methicillin-resistant Staphylococcus aureus. Eur J Clin Microbiol 1986; 5:702–709
    [Google Scholar]
  44. Brown DFJ, Kothari D. Antimicrobial-susceptibility testing of rapidly growing pathogenic bacteria. J Antimicrob Chemother 1978; 4:19–38
    [Google Scholar]
  45. Garrod LP, O’Grady F. Antibiotic and chemotherapy, 3rd edn.. Edinburgh: Livingstone; 1971451–464
    [Google Scholar]
  46. Stokes E, Ridgway GL, Wren MWD. Clinical microbiology, 7th edn.. London: Edward Arnold; 1993251
    [Google Scholar]
  47. Elek SD, Moryson C. Resistotyping of Staphylococcus aureus. J Med Microbiol 1973; 7:237–249
    [Google Scholar]
  48. Gelmi M, Foresti I, Ravizzola. et al. Antibiotic resistances and plasmids in Staphylococcus aureus from Italian hospitals. J Med Microbiol 1987; 23:111–118
    [Google Scholar]
  49. Aparicio P, Richardson J, Martin S, Vindel A, Marples RR, Cookson BD. An epidemic methicillin-resistant strain of Staphylococcus aureus in Spain. Epidemiol Infect 1992; 108:287–298
    [Google Scholar]
  50. Rosdahl VT, Rosendal K. Resistance to cadmium, arsenate and mercury among Danish strains of Staphylococcus aureus isolates from cases of bacteriaemia, 1957–1974. J Med Microbiol 1980; 13:383–391
    [Google Scholar]
  51. El Solh N, Fouace JM, Pillet J, Chabbert YA. Plasmid DNA content of multiresistant Staphylococcus aureus strains. Ann Microbiol 1981; 132B:131–156
    [Google Scholar]
  52. Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 1979; 7:1513–1523
    [Google Scholar]
  53. Birnboim HC. A rapid alkaline extraction method for the isolation of plasmid DNA. Methods in Enzymol 1983; 100:243–255
    [Google Scholar]
  54. Lech K, Brent R. Minipreps of plasmid DNA. In Ausubel FM, Brent R, Kingston RE. et al. (eds) Current protocols in molecular biology Boston: Wiley Interscience; 19911.6.1–1.6.6
    [Google Scholar]
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