@article{mbs:/content/journal/jmm/10.1099/00222615-41-6-393, author = "Debroy, Chitrita and Bright, B. D. and Wilson, R. A. and Yealy, Jeanne and Kumar, R. and Bhan, M. K.", title = "Plasmid-coded DNA fragment developed as a specific gene probe for the identification of enteroaggregative Escherichia coli", journal= "Journal of Medical Microbiology", year = "1994", volume = "41", number = "6", pages = "393-398", doi = "https://doi.org/10.1099/00222615-41-6-393", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-41-6-393", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "Summary Enteroaggregative Escherichia coli (EAggEC) is a recently discovered diarrhoeal pathogen implicated as a cause of persistent diarrhoea in children. EAggEC strains exhibit a characteristic pattern of adherence when incubated with HEp-2 cells. Because of the difficulty in identifying this group of bacteria, the epidemiological significance of this pathogen as a diarrhoeal agent has not been fully realised. A gene probe was developed from the 60-MDa plasmid associated with EAggEC strains that encodes the genes for adherence and fimbriae. The sensitivity of the gene probe was 93 % and the specificity 98 % for detecting EAggEC isolates and is potentially useful for diagnostic and epidemiological studies.", }