1887

Abstract

Summary

A sensitive rat endocarditis model which employed relatively small inocula (⩽ 10 cfu) was used to examine the role of coagulase in the pathogenesis of infection. Rats with indwelling, intracardiac catheters were challenged intravenously with three strains of The virulence of a coagulase-positive parental strain DU5808 was compared in terms of its ID50 for resected vegetations and catheters to that of two eoagulase-negative mutant strains (DU5809 and DU5814) which had undergone site-specific mutagenesis. Confidence intervals of infection rates were calculated and comparisons were performed of weights of infected vegetations, bacterial concentrations in vegetations and early mortality rates. From these virulence parameters, it was concluded that there were no differences in virulence among the three strains. The results from this investigation support the previous findings in mouse models of subcutaneous infection and mastitis, which indicated that coagulase production by does not appear to function as a virulence factor. Together, these data refute the longheld belief that coagulase is important in the pathogenesis of infection and indicate that other markers of virulence must be operative in diseases caused by the enduring pathogen .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-41-4-259
1994-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/41/4/medmicro-41-4-259.html?itemId=/content/journal/jmm/10.1099/00222615-41-4-259&mimeType=html&fmt=ahah

References

  1. Waldvogel FA. Staphylococcus aureus (including toxic shock syndrome). Mandell GL, Douglas RG, Bennett JE. Principles and practice of infectious diseases, 3rd edn.. New York: Churchill Livingstone; 19901489–1510
    [Google Scholar]
  2. Foster TJ, O’ Reilly M, Bramley AJ. Genetic studies of Staphylococcus aureus virulence factors. adström T, Eliasson I, Holder I, Ljungh A. Pathogenesis of wound and biomaterial-associated infections London: Springer-Verlag; 199035–46
    [Google Scholar]
  3. Mani N, Baddour LM, Offutt DQ, Vijaranakul U, Nadakavukaren MJ, Jayaswal RK. Autolysis-defective mutant of Staphylococcus aureus: pathological considerations, genetic mapping and electron microscopic studies. Infect Immun 1994; 62:1406–1409
    [Google Scholar]
  4. Hasegawa N, San Clemente CL. Virulence and immunity of Staphylococcus aureus BB and certain deficient mutants. Infect Immun 1978; 22:473–479
    [Google Scholar]
  5. Jonsson P, Lindberg M, Haraldsson I, Wadström T. Virulence of Staphylococcus aureus in a mouse mastitis model: studies of alpha hemolysin, coagulase, and protein A as possible virulence determinants with protoplast fusion and gene cloning. Infect Immun 1985; 49:765–769
    [Google Scholar]
  6. Masuda S. An efficient method for the isolation of a mutant with an extremely low producibility of coagulase from a Staphylococcus aureus strain. Microbiol Immunol 1983; 27:801–805
    [Google Scholar]
  7. Phonimdaeng P, O’Reilly M, Nowlan P, Bramley AJ, Foster TJ. The coagulase of Staphylococcus aureus 8325–4. Sequence analysis and virulence of site-specific coagulase-deficient mutants. Mol Microbiol 1990; 4:393–404
    [Google Scholar]
  8. O’Reilly M, de Azavedo JCS, Kennedy S, Foster TJ. Inactivation of the alpha-haemolysin gene of Staphylococcus aureus 8325–4 by site-directed mutagenesis and studies on the expression of its haemolysins. Microb Pathog 1986; 1:125–138
    [Google Scholar]
  9. Bramley AJ, Patel AH, O’Reilly M, Foster R, Foster TJ. Roles of alpha-toxin and beta-toxin in virulence of Staphylococcus aureus for the mouse mammary gland. Infect Immun 1989; 57:2489–2494
    [Google Scholar]
  10. Baddour LM, Lowrance C, Albus A, Lowrance JH, Anderson SK, Lee JC. Staphylococcus aureus microcapsule expression attenuates bacterial virulence in a rat model of experimental endocarditis. J Infect Dis 1992; 165:749–753
    [Google Scholar]
  11. SAS/STAT User’s Guide. Version 6 , 4th edn.. Cary, NC: SAS Institute; 1990865–866 1325–1350
    [Google Scholar]
  12. Miller RG. Beyond ANOVA, basics of applied statistics. New York: Wiley; 1986246
    [Google Scholar]
  13. Baddour LM, Christensen GD, Lowrance JH, Simpson WA. Pathogenesis of experimental endocarditis. Rev Infect Dis 1989; 11:452–463
    [Google Scholar]
  14. Albus A, Arbeit RD, Lee JC. Virulence of Staphylococcus aureus mutants altered in type 5 capsule production. Infect Immun 1991; 59:1008–1014
    [Google Scholar]
  15. Albus A, Liu MJ, Arbeit RD, Lee JC. Virulence studies of transposon-induced mutants of Staphylococcus aureus altered in capsule expression. Abstracts of the 90th Annual Meeting of the American Society for Microbiol1990 B-627
    [Google Scholar]
  16. Jeljaszewicz H, Switalski LM, Adlam C. Staphylocoagulase and clumping factor. Easmon CSF, Adlam C. Staphylococci and staphylococcal infections 2 London: Academic Press; 1983525–557
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-41-4-259
Loading
/content/journal/jmm/10.1099/00222615-41-4-259
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error