1887

Abstract

Summary

Arbitrarily primed polymerase chain reaction (AP-PCR) and ribotyping were compared in an investigation of an outbreak of infections. Twenty-five clinical isolates shown previously by other criteria to belong to two different groups, and nine randomly selected clinical isolates from other hospitals were investigated. Among the strains analysed, nine different RI rRNA gene restriction pattern fingerprints were observed. While similarity was detected between strains of the same group, these fingerprints differed clearly between the two groups defined in the outbreak. Two of the nine strains selected randomly had the same ribotype as those strains involved in the outbreak, whereas the remaining seven strains each had a different ribotype. When the strains were tested by AP-PCR with 0.25, 0.5 or 1 of M13 forward primer, 10 different profiles were obtained. However, 11 profiles were observed if two different primer concentrations (0.25 and 1 ) were used. It was concluded that ribotyping and AP-PCR exhibited a similar discriminatory power, although AP-PCR had the additional advantages of speed and simplicity.

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1994-10-01
2024-04-23
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References

  1. Castle M, Tenney JH, Weinstein MP, Eickhoff TC. Outbreak of a multiply resistant Acinetobacter in a surgical intensive care unit: epidemiology and control. Heart Lung 1978; 7:641–644
    [Google Scholar]
  2. Sherertz RJ, Sullivan ML. An outbreak of infections with Acinetobacter calcoaceticus in burn patients: contamination of patients’ mattresses. J Infect Dis 1985; 151:252–258
    [Google Scholar]
  3. Stone JW, Das BC. Investigation of an outbreak of infection with Acinetobacter calcoaceticus in a special care baby unit. J Hosp Infect 1986; 7:42–48
    [Google Scholar]
  4. Hartstein AI, Rashad AL, Liebler JM et al. Multiple intensive care unit outbreak of Acinetobacter calcoaceticus subspecies anitratus respiratory infection and colonization associated with contaminated, reusable ventilator circuits and resuscitation bags. Am J Med 1988; 85:624–631
    [Google Scholar]
  5. Cefai C, Richards J, Gould FK, McPeake P. An outbreak of Acinetobacter respiratory tract infection resulting from incomplete disinfection of ventilatory equipment. J Hosp Infect 1990; 15:177–182
    [Google Scholar]
  6. Vila J, Almela M, Jimenez de Anta MT. Laboratory investigation of hospital outbreak caused by two different multiresistant Acinetobacter calcoaceticus subsp. anitratus strains. J Clin Microbiol 1989; 27:1086–1089
    [Google Scholar]
  7. Towner KJ, Chopade BA. Biotyping of Acinetobacter calcoaceticus using the API 20NE system. J Hosp Infect 1987; 10:145–151
    [Google Scholar]
  8. Andrews HJ. Acinetobacter bacteriocin typing. J Hosp Infect 1986; 7:169–175
    [Google Scholar]
  9. Traub WH. Acinetobacter baumannii serotyping for delineation of outbreaks of nosocomial cross-infection. J Clin Microbiol 1989; 27:2713–2716
    [Google Scholar]
  10. Vieu JF, Bergogne-Bérézin E, Joly ML, Berthelot G, Fichelle A, Prevost C. Epidémiologie d’Acinetobacter calcoaceticus. Nouv Presse Med 1980; 9:3551–3552
    [Google Scholar]
  11. Alexander M, Ismail F, Jackman PJH, Noble WC. Fingerprinting Acinetobacter strains from clinical sources by numerical analysis of electrophoretic patterns. J Med Microbiol 1984; 18:55–64
    [Google Scholar]
  12. Dijkshoorn L, Michel MF, Degener JE. Cell envelope protein profiles of Acinetobacter calcoaceticus strains isolated in hospitals. J Med Microbiol 1987; 23:313–319
    [Google Scholar]
  13. Dijkshoorn L, van Vianen W, Degener JE, Michel MF. Typing of Acinetobacter calcoaceticus strains isolated from hospital patients by cell envelope protein profiles. Epidemiol Infect 1987; 99:659–667
    [Google Scholar]
  14. Vila J, Canales MA, Marcos MA, Gomez-Lus R, Jimenez de Anta MT. Molecular epidemiological analysis of nosocomial Acinetobacter baumannii isolates. Towner KJ, Bergogne-Bérézin E, Fewson CA. The biology of Acinetobacter: taxonomy, clinical importance, molecular biology, physiology, industrial relevance New York: Plenum Press; 199169–76
    [Google Scholar]
  15. Gerner-Smidt P. Frequency of plasmids in strains of Acinetobacter calcoaceticus. J Hosp Infect 1989; 14:23–28
    [Google Scholar]
  16. Kropec A, Hubner J, Daschner FD. Comparison of three typing methods in hospital outbreaks of Acinetobacter calcoaceticus infection. J Hosp Infect 1991; 23:133–141
    [Google Scholar]
  17. Allardet-Servent A, Bouziges N, Carles-Nurit MJ, Bourg G, Gouby A, Ramuz M. Use of low-frequency-cleavage restriction endonucleases for DNA analysis in epidemiological investigation of nosocomial bacterial infections. J Clin Microbiol 1989; 27:2057–2061
    [Google Scholar]
  18. Dijkshoorn L, Aucken HM, Gerner-Smidt P, Kaufmann ME, Ursing J, Pitt TL. Correlation of typing methods for Acinetobacter isolates from hospital outbreaks. J Clin Microbiol 1993; 31:702–705
    [Google Scholar]
  19. Gerner-Smidt P. Ribotyping of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. J Clin Microbiol 1992; 30:2680–2685
    [Google Scholar]
  20. Gouby A, Charles-Nurit MJ, Bouziges N, Bourg G, Mesnard R, Bouvet PJM. Use of pulsed-field gel electrophoresis for investigation of hospital outbreaks of Acinetobacter baumannii. J Clin Microbiol 1992; 30:1588–1591
    [Google Scholar]
  21. Graser Y, Klare I, Halle E et al. Epidemiological study of an Acinetobacter baumannii outbreak by using polymerase chain reaction fingerprinting. J Clin Microbiol 1993; 31:2417–2420
    [Google Scholar]
  22. Struelens MJ, Carlier E, Maes N, Serruys E, Quint WG, van Belkum A. Nosocomial colonization and infection with multiresistant Acinetobacter baumannii: outbreak delineation using DNA macrorestriction analysis and PCR-fingerprinting. J Hosp Infect 1993; 25:15–32
    [Google Scholar]
  23. Bouvet PJM, Grimont PAD. Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter Iwoffìi. Int J Syst Bacteriol 1986; 36:228–240
    [Google Scholar]
  24. Grimont F, Grimont PAD. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol 1986; 137B:165–175
    [Google Scholar]
  25. Hadorn K, Lenz W, Kayser FH, Shalit I, Krasemann C. Use of a ribosomal RNA gene probe for the epidemiological study of methicillin and ciprofloxacin resistant Staphylococcus aureus. Eur J Clin Microbiol Infect Dis 1990; 9:649–653
    [Google Scholar]
  26. Grimont PAD, Bouvet PJM. Taxonomy of Acinetobacter. Towner KJ, Bergogne-Bérézin E, Fewson CA. The biology of Acinetobacter: taxonomy, clinical importance, molecular biology, physiology, industrial relevance New York: Plenum Press; 199125–36
    [Google Scholar]
  27. Welsh J, Mc Clelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990; 18:7213–7218
    [Google Scholar]
  28. Vassart G, Georges M, Monsieur R, Brocas H, Lequarre AS, Christophe D. Sequence in M13 phage detects hypervariable satellites in human and animal DNA. Science 1987; 235:683–684
    [Google Scholar]
  29. Ryskov AP, Jincharadze AG, Prosnyak MI, Ivanov PL, Limborska SA. M13 phage DNA as a universal marker for DNA fingerprinting of animals, plants and microorganisms. FEBS Lett 1988; 233:388–392
    [Google Scholar]
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