Detection of by PCR amplification of gene Free

Abstract

Summary

Synthetic oligonucleotide primers were used in the polymerase chain reaction (PCR) to amplify a sequence of the gene, which encodes the surface protein antigen I/II of A DNA fragment of 192 bp was amplified from lysed cells or isolated DNA. With cells, the lower limit of detection was 4–40 cfu. With these primers, 13 reference and 50 clinical strains of were identified. Amplification of the 192-bp product was not demonstrated when 41 strains of other streptococcal and non-streptococcal species were tested. The gene PCR has potential for the rapid diagnosis of infections.

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1994-10-01
2024-03-29
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