1887

Abstract

Summary

Synthetic oligonucleotide primers were used in the polymerase chain reaction (PCR) to amplify a sequence of the gene, which encodes the surface protein antigen I/II of A DNA fragment of 192 bp was amplified from lysed cells or isolated DNA. With cells, the lower limit of detection was 4-40 cfu. With these primers, 13 reference and 50 clinical strains of were identified. Amplification of the 192-bp product was not demonstrated when 41 strains of other streptococcal and non-streptococcal species were tested. The gene PCR has potential for the rapid diagnosis of infections.

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/content/journal/jmm/10.1099/00222615-41-4-231
1994-10-01
2019-10-14
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-41-4-231
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