1887

Abstract

Summary

Clinical isolates (41) of spp. from three possible outbreaks of nosocomially-acquired infection were compared by pyrolysis mass spectrometry (PMS) and by a combined morphotyping and resistotyping (M-R typing) method. Both systems characterised all the isolates and distinguished one isolate of and another of C. from the 39 isolates of Results from both systems suggested that crossinfection with a single strain contributed to two of the outbreaks. In several instances, more than one strain of was found amongst multiple isolates from the same patient. PMS is a simple, rapid and objective technique capable of characterising isolates; discrimination was similar to M-R typing.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-41-2-98
1994-08-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/41/2/medmicro-41-2-98.html?itemId=/content/journal/jmm/10.1099/00222615-41-2-98&mimeType=html&fmt=ahah

References

  1. Hunter PR. A critical review of typing methods for Candida albicans and their applications. Crit Rev Microbiol 1991; 17:417–434
    [Google Scholar]
  2. Merz WG. Candida albicans strain delineation. Clin Microbiol Rev 1990; 3:321–334
    [Google Scholar]
  3. Stevens DA, Odds FC, Scherer S. Application of DNA typing methods to Candida albicans epidemiology and correlations with phenotype. Rev Infect Dis 1990; 12:258–266
    [Google Scholar]
  4. Vazquez JA, Beckley A, Sobel JD, Zervos M. Comparison of restriction enzyme analysis and pulsed-field gradient gel electrophoresis as typing systems for Candida albicans. J Clin Microbiol 1991; 29:962–967
    [Google Scholar]
  5. Pitt T. Burnt offerings or typing systems?. J Hosp Infect 1991; 17:157–158
    [Google Scholar]
  6. Magee JT, Hindmarch JM, Duerden , MacKenzie DWR. Pyrolysis mass spectrometry as a method for inter-strain discrimination of Candida albicans. J Gen Microbiol 1988; 134:2841–2847
    [Google Scholar]
  7. Phongpaichit S, Mackenzie DWR, Fraser CM. Strain differentiation of Candida albicans by morphotyping. Epidemiol Infect 1987; 99:421–428
    [Google Scholar]
  8. Hunter PR, . Fraser CAM. Application of a numerical index of discriminatory power to a comparison of four physio-chemical typing methods for Candida albicans. J Clin Microbiol 1989; 27:2156–2160
    [Google Scholar]
  9. Medcraft JW. Typing of Candida and the epidemiology of candiosis PhD thesis, University of Reading 1985
    [Google Scholar]
  10. Aries RE, Gutteridge CS, Ottley TW. Evaluation of a low-cost, automated pyrolysis mass spectrometer. J Anal Appl Pyr 1986; 91:81–98
    [Google Scholar]
  11. Orr K, Gould FK, Sisson P, Lightfoot NF, Freeman R, Burdess D. Rapid inter-strain comparison by pyrolysis mass spectrometry in nosocomial infection with Xanthomonas maltophilia. J Hosp Infect 1991; 17:187–195
    [Google Scholar]
  12. Bruatto M, Vidotto V, Marinuzzi G, Raiteri R, Sinicco A. Candida albicans biotypes in human immunodeficiency virus type 1-infected patients with oral candidiasis before and after antifungal therapy. J Clin Microbiol 1991; 29:726–730
    [Google Scholar]
  13. Soil DR, Galask R, Schmid J, Hanna C, Mac K, Morrow BC. Genetic dissimilarity of commensal strains of Candida spp. carried in different anatomical locations of the same healthy women. J Clin Microbiol 1991; 29:1702–1710
    [Google Scholar]
  14. Odds FC, Abbott AB. Modification and extension of tests for differentiation of Candida species and strains. Sabouraudia 1983; 21:79–81
    [Google Scholar]
  15. Brawner DL. Comparison between methods for serotyping of Candida albicans produces discrepancies in results. J Clin Microbiol 1991; 29:1020–1025
    [Google Scholar]
  16. Lehmann PF, Cowan LE, Jones RM, Ferencak WJ. Use of killer fungi and antifungal chemicals in characterization of yeast species and biotypes. Trans Br Mycol Soc 1987; 88:199–206
    [Google Scholar]
  17. Lehmann PF, Kemker BJ, Hsiao C-B, . Dev S. Isoenzyme biotypes of Candida species. J Clin Microbiol 1989; 27:2514–2521
    [Google Scholar]
  18. Dutton S, Penn CW. Biological attributes of colony-type variants of Candida albicans. J Gen Microbiol 1990; 135:3363–3372
    [Google Scholar]
  19. Rustchenko-Bulac EP, Sherman F, Hicks JB. Chromosomal rearrangements associated with morphological mutants provide a means for genetic variation of Candida albicans. J Bacteriol 1990; 172:1276–1283
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-41-2-98
Loading
/content/journal/jmm/10.1099/00222615-41-2-98
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error