%0 Journal Article %A Brennan, B. R. %A Oliver, J. J. %A Baird, G. D. %T Differences in the immune responses of mice and sheep to an aromatic-dependent mutant of Salmonella typhimurium %D 1994 %J Journal of Medical Microbiology, %V 41 %N 1 %P 20-28 %@ 1473-5644 %R https://doi.org/10.1099/00222615-41-1-20 %I Microbiology Society, %X Summary A live mutant aroA Salmonella serotype Typhimurium ovine strain (S25/1) could be cultured from tissues of mice for up to 90 days after oral infection. Following vaccination, high levels of Salmonella-specific serum IgM, IgG and IgA were produced in addition to high levels of specific intestinal IgA. Moreover, there was also evidence of Salmonella-specific cell-mediated immunity in vaccinated mice in the form of strong delayed-type hypersensitivity and the production of interferon-gamma (IFN-τ) by spleen cells stimulated with Salmonella antigen. The aroA strain was also recovered from the mesenteric lymph nodes and most tissues examined from sheep vaccinated by the oral route. Salmonella-specific IgM was detected in the serum; however, specific IgG responses were very low and there was an absence of specific copro-antibody. Although strong Salmonella-specific lymphocyte proliferative responses were detected, they did not result in the production of IFN-τ and flow cytometric analysis revealed that the proliferating cells were predominantly B lymphocytes. Despite the absence of strong vaccine-specific immune responses in vaccinated sheep compared with those seen in mice, both mice and sheep were protected against challenge with virulent wild-type strain S25/1. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-41-1-20