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Volume 41, Issue 1

Agglutination of “” by lectins No Access

Abstract

Summary

The agglutination of 218 clinical isolates and three ATCC type strains of “” was tested with 25 different lectins from plants and fungi. An agglutination reaction with one or more lectins was observed with 42 isolates when the cells were untreated. After trypsinisation of the bacteria, 109 strains yielded a positive reaction and after boiling the bacterial cells at pH 2, 218 isolates were agglutinated. As an overall result of our experiments with untreated, trypsinised and boiled cells, 17, 37 and 45 different agglutination patterns, respectively, were obtained. The lectins from and agglutinated isolates belonging only to Lancefield group C, being non-reactive with other isolates. These lectins were also found to be specific for “large colony type” streptococci of group C. The use of lectin agglutination in epidemiological and ecological studies of “S. ” is discussed.

  • Received:
  • Accepted:
  • Published Online:
© 1994 The Pathological Society of Great Britain and Ireland
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1994-07-01
2025-04-21
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References

  1. Guthof O. Über pathogene “vergrünende Streptokokken”. Streptokokken-Befunde bei dentogenen Abszessen und Infiltraten im Bereich der Mundhöhle. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt 1 Orig 1956; 166:553–564
     [Google Scholar]
  2. Guthof O, Colman G, Williams REO. Taxonomy of some human viridans Streptococci. In: Wannamaker LW, Matsen JM. Streptococci and streptococcal diseases: recognition, understanding and management New York: Academic Press; 1972281–299
     [Google Scholar]
  3. Facklam RR. The major differences in the American and British Streptococcus taxonomy schemes with special reference to “Streptococcus milleri”. Eur J Clin Microbiol 1984; 3:91–93
     [Google Scholar]
  4. Coykendall AL, Wesbecher PM, Gustafson KB. “Streptococcus milleri”, Streptococcus constellatus, and Streptococcus intermedius are later synonyms of Streptococcus anginosus. Int J Syst Bacteriol 1987; 37:222–228
     [Google Scholar]
  5. Whiley RA, Hardie JM. DNA-DNA hybridization studies and phenotypic characteristics of strains within the “Streptococcus milleri” group. J Gen Microbiol 1989; 135:2623–2633
     [Google Scholar]
  6. Whiley RA, Beighton D. Emended descriptions of Streptococcus constellatus, Streptococcus intermedius and Streptococcus anginosus as distinct species. Int J Syst Bacteriol 1991; 41:1–5
     [Google Scholar]
  7. Skerman VBD, McGown V, Sneath PHA. Approved lists of bacterial names. Int J Syst Bacteriol 1980; 30:225–420
     [Google Scholar]
  8. Piscitelli SC, Shwed J, Schreckenberger P, Danziger LH. “Streptococcus milleri” group: renewed interest in an elusive pathogen. Eur J Clin Microbiol Infect Dis 1992; 11:491–498
     [Google Scholar]
  9. Drucker DB, Lee SM. Fatty acid fingerprints of “Streptococcus milleri”, Streptococcus mitis, and related species. Int J Syst Bacteriol 1981; 31:219–225
     [Google Scholar]
  10. Drucker DB, Lee SM. Possible heterogeneity of “Streptococcus milleri” determined by DNA mol% (guanine + cytosine) measurement and physiological characterization. Microbios 1983; 38:151–157
     [Google Scholar]
  11. Aluyi HS, Drucker DB. Trimethylsilyl-sugar profiles of “Streptococcus milleri” and Streptococcus mitis. J Appl Bacteriol 1983; 54:391–397
     [Google Scholar]
  12. Lotan R, Skutelsky E, Danon D, Sharon N. The purification, composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea). J Biol Chem 1975; 250:8518–8523
     [Google Scholar]
  13. Peumans WJ, Nsimba-Lubaki M, Peeters B, Broekaert WF. Isolation and partial characterization of a lectin from ground elder (Aegopodium podagraria) rhizomes. Planta 1985; 164:75–82
     [Google Scholar]
  14. Van Damme EJM, Goldstein IJ, Peumans WJ. A comparative study of related mannose-binding lectins from Amaryllidaceae and Alliaceae species. Phytochemistry 1991; 30:509–514
     [Google Scholar]
  15. Peumans WJ, Nsimba-Lubaki M, Carlier AM, Van Driessche E. A lectin from Bryonia dioica root stocks. Planta 1984; 160:222–228
     [Google Scholar]
  16. Broekaert WF, Peumans WJ, Allen AK. Carbohydrate binding proteins from Datura stramonium seeds. In: Bøg-Hansen TC, Breborowicz J. Lectins 4 Berlin: Walter de Gruyter Co.; 1985481–489
     [Google Scholar]
  17. Kawaguchi T, Matsumoto I, Osawa T. Studies on hemagglutinins from Maackia amurensis seeds. J Biol Chem 1974; 249:2786–2792
     [Google Scholar]
  18. Wantyghem J, Goulut C, Frenoy JP, Turpin E, Goussault Y. Purification and characterization of Robinia pseudoacacia seed lectins. Biochem J 1986; 237:483–489
     [Google Scholar]
  19. Kellens JTC, Peumans WJ. Occurrence of lectins in different strains of Rhizoctonia solani. In: Kocourek J, Freed DJL. Lectins 7 St Louis: Sigma Chemical Co.; 199057–62
     [Google Scholar]
  20. Kellens JTC, Goldstein IJ, Peumans WJ. Lectins in different members of the Sclerotiniaceae. Mycol Res 1992; 96:495–502
     [Google Scholar]
  21. Kaku H, Peumans WJ, Goldstein IJ. Isolation and characterization of a second lectin (SNA-II) present in elderberry (Sambucus nigra L.) bark. Arch Biochem Biophvs 1990; 277255–262
     [Google Scholar]
  22. Cammue BPA, Peeters B, Peumans WJ. A new lectin from tulip (Tulipa) bulbs. Planta 1986; 169:583–588
     [Google Scholar]
  23. Kurokawa T, Tsuda M, Sugino Y. Purification and characterization of a lectin from Wisteria floribunda seeds. J Biol Chem 1976; 251:5686–5693
     [Google Scholar]
  24. Nagata Y, Burger MM. Wheat germ agglutinin. Molecular characteristics and specificity for sugar binding. J Biol Chem 1974; 249:3116–3122
     [Google Scholar]
  25. Agrawal BBL, Goldstein IJ. Protein-carbohydrate interaction. VI. Isolation of concanavalin A by specific adsorption on cross-linked dextran gels. Biochim Biophvs Acta 1967; 147:262–271
     [Google Scholar]
  26. Etzler ME, Kabat EA. Purification and characterization of a lectin (plant hemagglutinin) with blood group A specificity and Dolichos biflorus. Biochemistry 1970; 9:869–877
     [Google Scholar]
  27. Ziska P, Franz H, Kindt A. The lectin I from Viscum album L. purification by biospecific affinity chromatography. Experientia 1978; 34:123–125
     [Google Scholar]
  28. Kellens JTC, Jacobs JA, Peumans WJ, Stobberingh EE. The agglutination of β-haemolytic streptococci by lectins. J Med Microbiol 1993; 39:440–445
     [Google Scholar]
  29. Gossling J. Occurrence and pathogenicity of the “Streptococcus milleri” group. Rev Infect Dis 1988; 10:257–285
     [Google Scholar]
  30. Wagner M. Interaction of wheat-germ agglutinin with streptococci and streptococcal cell wall polymers. Immunobiology 1979; 156:57–64
     [Google Scholar]
  31. Slifkin M, Doyle RJ. Lectins and their application to clinical microbiology. Clin Microbiol Rev 1990; 3:197–218
     [Google Scholar]
  32. Köhler W, Nagai T. Reactions of the lectin anti-AHP from the edible snail Helix pomatia with N-acetyl-D-galactosamine of streptococci. Kitasato Arch Exp Med 1989; 62:107–113
     [Google Scholar]
  33. Lütticken R, Wendorff U, Lütticken D, Johnson EA, Wannamaker LW. Studies on streptococci resembling “Streptococcus milleri” and on an associated surface-protein antigen. J Med Microbiol 1978; 11:419–431
     [Google Scholar]
  34. Uhlenbruck G, Pardoe Gl, Prokop O. Untersuchungen zur Spezifität der Helixagglutinine. In: Perlick E, Plenert W, Prokop O. Fortschritte der Hämatologie Leipzig: Johann Ambrosius Barth; 197041–69
     [Google Scholar]
/content/journal/jmm/10.1099/00222615-41-1-14
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Agglutination of “Streptococcus milleri” by lectins
J. Med. Microbiol. 41, 14 (1994); https://doi.org/10.1099/00222615-41-1-14
/content/journal/jmm/10.1099/00222615-41-1-14
/content/journal/jmm/10.1099/00222615-41-1-14
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