1887

Abstract

Summary

Enrichment culture (EC) in modified buffered peptone water followed by immunomagnetic separation (IMS) with magnetic beads coated with an antibody against 0157 (Dynabeads anti-is. O157; Dynal, Oslo) was compared with direct culture on cefixime rhamnose sorbitol MacConkey agar (CR-SMAC) and cefixime tellurite sorbitol MacConkey agar (CT-SMAC) for the isolation of . O157 from bovine faeces. When used to examine bovine faecal suspensions inoculated with 12 different strains of . O157, EC-IMS was . 100-fold more sensitive for detection of the organism than direct culture on either medium. During monitoring of a dairy herd, . O157 was isolated from 84 (8·2 %) of 1024 rectal swabs taken from cattle over a 4-month period; 23 (27·4 % of the 84 strains were isolated by both direct culture and IMS (15 of the 23 were isolated on both media, five on CT-SMAC only and three on CR-SMAC only), whereas 61 (72·6%) strains were isolated by IMS only. IMS is a sensitive and simple technique for the isolation of . O157 from bovine faecal samples and should prove useful in elucidating further the epidemiology of this organism.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-40-6-424
1994-06-01
2022-05-22
Loading full text...

Full text loading...

/deliver/fulltext/jmm/40/6/medmicro-40-6-424.html?itemId=/content/journal/jmm/10.1099/00222615-40-6-424&mimeType=html&fmt=ahah

References

  1. Chapman PA, Wright DJ, Norman P. Verotoxin-producing Escherichia coli infections in Sheffield: cattle as a possible source. Epidemiol Infect 1989; 102:439–445
    [Google Scholar]
  2. Chapman PA, Siddons CA, Wright DJ, Norman P, Fox J, Crick E. Cattle as a possible source of verocytotoxin-producing Escherichia coli O157 infections in man. Epidemiol Infect 1993; 111:439–447
    [Google Scholar]
  3. Chapman PA, Wright DJ, Higgins R. Untreated milk as a source of verotoxigenic E coli O157. Vet Rec 1993; 133:171–172
    [Google Scholar]
  4. March SB, Ratnam S. Sorbitol-MacConkey medium for the detection of Escherichia coli O157: H7 associated with hemorrhagic colitis. J Clin Microbiol 1986; 23:869–872
    [Google Scholar]
  5. Chapman PA, Siddons CA, Zadik PM, Jewes L. An improved selective medium for the isolation of Escherichia coli O157. J Med Microbiol 1991; 35:107–110
    [Google Scholar]
  6. Zadik PM, Chapman PA, Siddons CA. Use of tellurite for the selection of verocytotoxigenic Escherichia coli O157. J Med Microbiol 1993; 39:155–158
    [Google Scholar]
  7. Chapman PA. Evaluation of commercial latex slide test for identifying Escherichia coli O157. J Clin Pathol 1989; 42:1109–1110
    [Google Scholar]
  8. Chapman PA, Daly CM. Evaluation of a non-radioactive trivalent DNA probe (LTh, ST la, ST lb) for detecting enterotoxigenic Escherichia coli . J Clin Pathol 1993; 46:309–312
    [Google Scholar]
  9. Chapman PA, Jewes L, Siddons CA, Norman P, George SL. Verotoxin-producing Escherichia coli infections in Sheffield during 1989. PHLS Microbiol Digest 1990; 7:163–166
    [Google Scholar]
  10. Borczyk AA, Karmali MA, Lior H. Duncan LMC. Bovine reservoir for verotoxin-producing Escherichia coli O157:H7. Lancet 1987; 1:98
    [Google Scholar]
  11. Martin ML, Shipman LD, Wells JG. Isolation of Escherichia coli O157: H7 from dairy cattle associated with two cases of haemolytic uraemic syndrome. Lancet 1986; 2:1043
    [Google Scholar]
  12. Ostroff SM, Griffin PM, Tauxe RV. A statewide outbreak of Escherichia coli O157 infections in Washington state. Am J Epidemiol 1990; 132:239–247
    [Google Scholar]
  13. Wells JG, Shipman LD, Greene KD. Isolation of Escherichia coli serotype O157: H7 and other shiga-like toxin-producing E. coli from dairy cattle. J Clin Microbiol 1991; 29:985–989
    [Google Scholar]
  14. Fratamico PM, Schultz FJ, Buchanan RL. Rapid isolation of Escherichia coli O157:H7 from enrichment cultures of foods using an immunomagnetic separation method. Food Microbiol 1992; 9:105–113
    [Google Scholar]
  15. Okrend AJG, Bonnie ER, Lattuada CP. Isolation of Escherichia coli O157:H7 using O157 specific antibody coated magnetic beads. J Food Protect 1992; 55:214–217
    [Google Scholar]
  16. Padhye NV, Doyle MP. Production and characterization of a monoclonal antibody specific for enterohemorrhagic Escherichia coli of serotypes O157:H7 and O26:H11. J Clin Microbiol 1991; 29:99–103
    [Google Scholar]
  17. Sernowski LP, Ingham SC. Frequency of false presumptive positive results obtained using a commercial ELISA kit to screen retail ground beef for Escherichia coli O157:H7. J Food Protect 1992; 55:846
    [Google Scholar]
  18. Szabo RAE, Todd E, MacKenzie J, Parrington L, Armstrong A. Increased sensitivity of the rapid hydrophobic grid membrane filter enzyme labelled antibody procedure for Escherichia coli O157 detection in foods and bovine faeces. Appl Environ Microbiol 1990; 56:3546–3549
    [Google Scholar]
  19. Doyle MP, Schoeni JL. Isolation of Escherichia coli O157:H7 from retail fresh meats and poultry. Appl Environ Microbiol 1987; 83:2394–2396
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-40-6-424
Loading
/content/journal/jmm/10.1099/00222615-40-6-424
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error