Skip to content
1887

Abstract

Summary

Adenovirus isolates from 52 patients with ocular infection over a 3-year period were typed by restriction endonuclease analysis in a clinical laboratory. The results indicated that adenovirus type 8 was the most common cause of adenovirus eye infection during this period, being responsible for 42 (81 %) of the 52 cases. Of 42 adenovirus type 8 isolates, 22 showed variant patterns by restriction endonuclease analysis and required multiple enzyme digests for identification. These isolates were readily identified by neutralisation tests

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-40-6-385
1994-06-01
2025-01-14
Loading full text...

Full text loading...

References

  1. Mahafzah AM, Landry ML. Evaluation of immunofluorescent reagents, centrifugation, and conventional cultures for the diagnosis of adenovirus infection. Diagn Microbiol Infect Dis 1989; 12:407–411
    [Google Scholar]
  2. Fife KH, Ashley R, Shields AF, Salter D, Meyers JD, Corey L. Comparison of neutralization and DNA restriction enzyme methods for typing clinical isolates of human adenovirus. J Clin Microbiol 1985; 22:95–100
    [Google Scholar]
  3. Adrian T, Wadell G, Hierholzer JC, Wigand R. DNA restriction analysis of adenovirus prototypes 1 to 41. Arch Virol 1986; 91:277–290
    [Google Scholar]
  4. Hierholzer JC, Bingham PG. Vero Microcultures for adenovirus neutralization tests. J Clin Microbiol 1978; 7:499–506
    [Google Scholar]
  5. Kemp MC, Hierholzer JC, Cabradilla CP, Obijeski JF. The changing etiology of epidemic keratoconjunctivitis: anti-genic and restriction enzyme analyses of adenovirus types 19 and 37 isolated over a 10-year period. J Infect Dis 1983; 148:24–33
    [Google Scholar]
  6. Kemp MC, Hierholzer JC. Three adenovirus type 8 genome types denned by restriction enzyme analysis: prototype stability in geographically separated populations. J Clin Microbiol 1986; 23:469–474
    [Google Scholar]
  7. Fife KH, Ashley R, Corey L. Isolation and characterization of six new genome types of human adenovirus types 1 and 2. J Clin Microbiol 1985; 21:20–23
    [Google Scholar]
  8. Wadell G, de Jong JC. Restriction endonucleases in identification of a genome type of adenovirus 19 associated with keratoconjunctivitis. Infect Immum 1980; 27:292–296
    [Google Scholar]
  9. Wadell G, Cooney MK, de Costa Linhares A et al. Molecular epidemiology of adenoviruses : global distribution of adenovirus 7 genome types. J Clin Microbiol 1985; 21:403–408
    [Google Scholar]
  10. Webb DH, Shields AF, Fife KH. Genomic variation of adenovirus type 5 isolates recovered from bone marrow transplant recipients. J Clin Microbiol 1987; 25:305–308
    [Google Scholar]
  11. Fujii SI, Nakozono M, Sawada H et al. Restriction endonuclease cleavage analysis of adenovirus type 8 : two new subtypes from patients with epidemic keratoconjunctivitis in Sapporo, Japan. JpnJMedSci Biol 1983; 36:307–313
    [Google Scholar]
  12. Hammond GW, Mauthe G, Joshua J, Hannan CK. Examination of uncommon clinical isolates of human adenoviruses by restriction endonuclease analysis. J Clin Micro-biol 1985; 21:611–616
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-40-6-385
Loading
/content/journal/jmm/10.1099/00222615-40-6-385
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error