1887

Abstract

Summary

Immunoblotting sera from cases of or . endocarditis against an extract from . strain MUCOB 263 had identified three immunodominant antigenic bands at 190, 200 and 220 kDa. A lambda ZAPII DNA library was produced from the sheared genomic DNA of . MUCOB 263 and six identical positive clones were identified when this library was screened with serum from a patient with endocarditis caused by a bacterium from the mutans group of streptococci. On subcloning and sequencing, a protein containing 1548 amino acids was identified with a 99·2 % homology to the SpaA antigen of . and 68·4 % homology to the PAc antigen of . .

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1994-05-01
2022-01-22
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