RT Journal Article SR Electronic(1) A1 Tikoo, Anjali A1 Singh, D. V. A1 Sanyal, S. C.YR 1994 T1 Influence of animal passage on haemolysin and enterotoxin production in Vibrio cholerae O1 biotype El Tor strains JF Journal of Medical Microbiology, VO 40 IS 4 SP 246 OP 251 DO https://doi.org/10.1099/00222615-40-4-246 PB Microbiology Society, SN 1473-5644, AB Summary Of 43 strains of Vibrio cholerae Ol biotype El Tor isolated over a span of almost three decades (1964–1990) from stools of children and adults with diarrhoea (25 isolates) and from sewage (three) and water from the river Ganges (15) examined for production of haemolysin and its correlation with enterotoxin production, 17 isolates showed haemolysis. The majority of isolates (26), including 68 % of diarrhoeal and 50 % of environmental origin, were non-haemolytic. The titre of haemolysin produced was 4-16 HU/ml, irrespective of the source of isolation. Haemolytic strains caused significantly more fluid accumulation than the non-haemolytic strains in the rabbit ileal loop (RIL) test. Twenty nine (67·4) V. cholerae biotype El Tor isolates—all the haemolytic and most (61·5 %) of the non-haemolytic isolates tested—caused fluid accumulation. The remaining non-haemolytic strains that caused little or no accumulation of fluid did so after one to four consecutive passage(s) through RIL without change in haemolytic character; these strains required more consecutive passage through rabbit gut to show haemolysis. All these strains reverted to their original non-haemolytic character on repeated subculture or on storage in the laboratory but continued to show enterotoxic activity. The present study indicated that El Tor haemolysin is not responsible for fluid accumulation in rabbit gut., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-40-4-246