Of 43 strains of Ol biotype El Tor isolated over a span of almost three decades (1964–1990) from stools of children and adults with diarrhoea (25 isolates) and from sewage (three) and water from the river Ganges (15) examined for production of haemolysin and its correlation with enterotoxin production, 17 isolates showed haemolysis. The majority of isolates (26), including 68% of diarrhoeal and 50% of environmental origin, were non-haemolytic. The titre of haemolysin produced was 4–16 HU/ml, irrespective of the source of isolation. Haemolytic strains caused significantly more fluid accumulation than the non-haemolytic strains in the rabbit ileal loop (RIL) test. Twenty nine (67·4) biotype El Tor isolates—all the haemolytic and most (61·5%) of the non-haemolytic isolates tested—caused fluid accumulation. The remaining non-haemolytic strains that caused little or no accumulation of fluid did so after one to four consecutive passage(s) through RIL without change in haemolytic character; these strains required more consecutive passage through rabbit gut to show haemolysis. All these strains reverted to their original non-haemolytic character on repeated subculture or on storage in the laboratory but continued to show enterotoxic activity. The present study indicated that El Tor haemolysin is not responsible for fluid accumulation in rabbit gut.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error