Adult Sprague-Dawley rats infected intrabronchially with Bordetella pertussis strain 18-323 encased in agarose beads (BP-beads), developed a paroxysmal cough and leucocytosis, both of which peaked at around day 10. When animals were exposed to ether for 2 min after delivery of the beads, there was an enhancement of the number of subsequent coughing episodes. Inclusion of carrageenan in the beads also enhanced coughing. Control rats, given sterile beads or left untreated, showed only a low level of coughing or no coughing, depending upon their source. Rats challenged by the same route with heat-killed B. pertussis in beads, or with live organisms in suspension (without beads) showed no cough induction or leucocytosis. However, intranasal delivery of B. pertussis suspension gave rise to a moderate amount of coughing and leucocytosis. Serum IgG responses to B. pertussis antigens were greatest in rats infected with BP-beads and antibodies against both pertussis toxin and filamentous haemagglutinin were detected. Since the rat is the only conveniently accessible laboratory animal species in which B. pertussis induces an intermittent paroxysmal cough, as in man, it merits further study for determining the mechanisms of pathogenesis and immunity in pertussis.
AshworthLAE,
FitzgeorgeRB,
IronsLI,
MorganCP,
RobinsonA.
Rabbit nasopharyngeal colonization by Bordetella pertussis : the effects of immunization on clearance and on serum and nasal antibody levels. J Hyg1982; 88:475–486
WoodsDE,
FranklinR,
CryzSJ,
GanssM,
PeppierM,
EwanowichC. Development of a rat model for respiratory infection with Bordetella pertussis
. Infect Immun1989; 57:1018–1024
CashHA,
WoodsDE,
McCulloughB,
JohansonWG,
BassJA.
A rat model of chronic respiratory infection with Pseudomonas aeruginosa
. Am Rev Respir Dis1979; 119:453–459
PerkinsFT,
SheffieldFW,
OutschoornAS,
HemsleyDA.
An international collaborative study on the measurement of the opacity of bacterial suspensions. J Biol Standard1973; 1:1–10
ImaizumiA,
SuzukiY,
OnoS,
SatoH,
SatoY.
Effect of heptakis (2,6-O-dimethyl) βcyclodextrin on the production of pertussis toxin by Bordetella pertussis
. Infect Immun1983; 41:1138–1143
RichardsonPS. The function of the airway mucosa. DonachieW,
GriffithsE,
StephenJ.
Bacterial infections of respiratory and gastrointestinal mucosae Special publications of the Society for General Microbiology 24 Oxford: IRL Press; 19881–8
DomenighiniM,
RelmanD,
CapiauC.
Genetic characterization of Bordetella pertussis filamentous haemag-glutinin: a protein processed from an unusually large precursor. Mol Microbiol1990; 4:787–800