1887

Abstract

Summary

Adult Sprague-Dawley rats infected intrabronchially with strain 18-323 encased in agarose beads (BP-beads), developed a paroxysmal cough and leucocytosis, both of which peaked at around day 10. When animals were exposed to ether for 2 min after delivery of the beads, there was an enhancement of the number of subsequent coughing episodes. Inclusion of carrageenan in the beads also enhanced coughing. Control rats, given sterile beads or left untreated, showed only a low level of coughing or no coughing, depending upon their source. Rats challenged by the same route with heat-killed in beads, or with live organisms in suspension (without beads) showed no cough induction or leucocytosis. However, intranasal delivery of suspension gave rise to a moderate amount of coughing and leucocytosis. Serum IgG responses to antigens were greatest in rats infected with BP-beads and antibodies against both pertussis toxin and filamentous haemagglutinin were detected. Since the rat is the only conveniently accessible laboratory animal species in which induces an intermittent paroxysmal cough, as in man, it merits further study for determining the mechanisms of pathogenesis and immunity in pertussis.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-40-3-205
1994-03-01
2024-11-10
Loading full text...

Full text loading...

/deliver/fulltext/jmm/40/3/medmicro-40-3-205.html?itemId=/content/journal/jmm/10.1099/00222615-40-3-205&mimeType=html&fmt=ahah

References

  1. Wardlaw AC, Parton R. Pathogenesis and immunity in pertussis Chichester: Wiley; 198875–271
    [Google Scholar]
  2. Parton R. Changing perspectives on pertussis and pertussis vaccination. Rev MedMicrobiol 1991; 2:121–128
    [Google Scholar]
  3. Pittman M, Furman BL, Wardlaw AC. Bordetella pertussis respiratory tract infection in the mouse: pathophysio-logical responses. J Infect Dis 1980; 142:56–66
    [Google Scholar]
  4. Ashworth LAE, Fitzgeorge RB, Irons LI, Morgan CP, Robinson A. Rabbit nasopharyngeal colonization by Bordetella pertussis : the effects of immunization on clearance and on serum and nasal antibody levels. J Hyg 1982; 88:475–486
    [Google Scholar]
  5. Stanbridge TN, Preston NW. Experimental pertussis infection in the marmoset : type specificity of active immunity. J Hyg 1974; 72:213–228
    [Google Scholar]
  6. Sato Y, Sato H. Animal models of pertussis. Wardlaw AC, Parton R. Pathogenesis and immunity in pertussis Chichester: Wiley; 1988309
    [Google Scholar]
  7. Weiss AA, Goodwin MSM. Lethal infection by Bordetella pertussis mutants in the infant mouse model. Infect Immun 1989; 57:3757–3764
    [Google Scholar]
  8. Hornibrook JW, Ashburn LL. A study of experimental pertussis in the young rat. Public Health Rep 1939; 54:439–444
    [Google Scholar]
  9. Woods DE, Franklin R, Cryz SJ, Ganss M, Peppier M, Ewanowich C. Development of a rat model for respiratory infection with Bordetella pertussis . Infect Immun 1989; 57:1018–1024
    [Google Scholar]
  10. Cash HA, Woods DE, McCullough B, Johanson WG, Bass JA. A rat model of chronic respiratory infection with Pseudomonas aeruginosa . Am Rev Respir Dis 1979; 119:453–459
    [Google Scholar]
  11. Wardlaw AC, Hall E, Parton R. Coughing rat model of pertussis. Biologicals 1993; 21:27–29
    [Google Scholar]
  12. Perkins FT, Sheffield FW, Outschoorn AS, Hemsley DA. An international collaborative study on the measurement of the opacity of bacterial suspensions. J Biol Standard 1973; 1:1–10
    [Google Scholar]
  13. Weiss AA, Hewlett EL, Myers GA, Falkow S. Tn5-induced mutations affecting virulence factors of Bordetella pertussis . Infect Immun 1983; 42:33–41
    [Google Scholar]
  14. Imaizumi A, Suzuki Y, Ono S, Sato H, Sato Y. Effect of heptakis (2,6-O-dimethyl) βcyclodextrin on the production of pertussis toxin by Bordetella pertussis . Infect Immun 1983; 41:1138–1143
    [Google Scholar]
  15. Christodoulides M, Sidey FM, Parton R, Stewart-Tull DES. Acellular pertussis vaccine prepared by a simple extraction and toxoiding procedure. Vaccine 1987; 5:199–207
    [Google Scholar]
  16. Kohn FD, Barthold SW. Biology and diseases of rats. Fox JG, Bennett JC, Loen FM. Laboratory animal medicine Orlando: Academic Press; 1984100
    [Google Scholar]
  17. Richardson PS. The function of the airway mucosa. Donachie W, Griffiths E, Stephen J. Bacterial infections of respiratory and gastrointestinal mucosae Special publications of the Society for General Microbiology 24 Oxford: IRL Press; 19881–8
    [Google Scholar]
  18. Domenighini M, Relman D, Capiau C. Genetic characterization of Bordetella pertussis filamentous haemag-glutinin: a protein processed from an unusually large precursor. Mol Microbiol 1990; 4:787–800
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-40-3-205
Loading
/content/journal/jmm/10.1099/00222615-40-3-205
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error