1887

Abstract

SUMMARY:

Affinity chromatography on polymyxin B-Sepharose 4B is one of the most commonly used methods for the removal of contaminating lipopolysaccharides (LPS). However, the LPS of spp. do not bind to polymyxin B. An affinity chromatography method with an anti-O antigen of LPS monoclonal antibody as immunosorbent was developed. The method produced a 1000-fold reduction in the LPS content of the cytoplasmic fraction of . The eluted proteins retained their antigenicity. The method, which uses mild physiological conditions, is simple, effective and reproducible.

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/content/journal/jmm/10.1099/00222615-40-3-174
1994-03-01
2019-12-11
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http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-40-3-174
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