1887

Abstract

SUMMARY

The interaction of human monocytes or monocyte-derived macrophages and yeast-form was studied . Yeast cells were readily ingested by adherent monocytes or macrophages. Multiplication of . , measured by growth as colony forming units (cfu) on a supplemented medium with good plating efficiency, was greater in monocyte co-cultures compared to the number of cfu obtained from complete tissue-culture medium (CTCM). Multiplication increased with time in macrophage co-cultures, e.g., from two-six-fold in 24 h to nine-fold in 72 h. Microscopic observations indicated that ingested yeast cells multiplied inside macrophages. When monocytes were treated with supernate cytokines (CK) from concanavalin-A-stimulated mononuclear cells, then co-cultured with . , multiplication was significantly inhibited compared with control monocyte co-cultures. Treatment of macrophages—derived from monocytes by culture for 3 days—for a further 3 days with CK resulted in maximal inhibition of multiplication over the subsequent 72 h. Similarly, when monocyte-derived macrophages (after culture for 7 days) were treated for 3 days with recombinant human y-interferon (IFN; 300 U/ml) or CK they restricted multiplication of . by 65% and 95%, respectively, compared with control macrophages. Antibody to IFN abrogated the effect of IFN or CK treatment. These findings show that ingested . can multiply in human monocytes or macrophages and that this multiplication can be restricted by activated monocytes or macrophages.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-40-3-159
1994-03-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/40/3/medmicro-40-3-159.html?itemId=/content/journal/jmm/10.1099/00222615-40-3-159&mimeType=html&fmt=ahah

References

  1. Restrepo A, Greer DL, Vasconellos M. Paracoccidioido-mycosis: a review. Rev Med Vet Mycol 1973; 8:97–123
    [Google Scholar]
  2. McEwen JG, Bedoya V, Patino MM, Salazar ME, Restrepo A. Experimental murine paracoccidioidomycosis induced by the inhalation of conidia. J Med Vet Mycol 1987; 25:165–175
    [Google Scholar]
  3. Brummer E, Hanson LH, Restrepo A, Stevens DA. Intracellular multiplication of Paracoccidioides brasiliensis in macrophages: killing and restriction of multiplication by activated macrophages. Infect Immun 1989; 57:2289–2294
    [Google Scholar]
  4. Musatti CC, Reskallah MT, Mendes E, Mendes NF. In vivo and in vitro evaluation of cell-mediated immunity in patients with paracoccidioidomycosis. Cell Immunol 1976; 24:365–378
    [Google Scholar]
  5. Restrepo A, Restrepo M, de Restrepo F, Aristizabel LH, Moncada LH, Velez H. Immune responses in paracoccidioidomycosis. A controlled study of 16 patients before and after treatment. Sabouraudia 1978; 16:151–163
    [Google Scholar]
  6. Furtado JS, De Brito T, Freymuller E. The structure and reproduction of Paracoccidioides brasiliensis in human tissues. Sabouraudia 1967; 5:226–229
    [Google Scholar]
  7. Moscardi-Bacchi M, Soares A, Mendes R, Marques S, Franco M. In situ localization of T lymphocyte subsets in human paracoccidioidomycosis. J Med Vet Mycol 1989; 27:149–158
    [Google Scholar]
  8. Restrepo A, Jimenez BE. Growth of Paracoccidioides brasiliensis yeast phase in a chemically defined medium. J Clin Microbiol 1980; 12:279–281
    [Google Scholar]
  9. Restrepo A, Cano LE, de Bedout C, Brummer E, Stevens DA. Comparison of various techniques for determining viability of Paracoccidioides brasiliensis yeast-form cells. J Clin Microbiol 1982; 16:209–211
    [Google Scholar]
  10. Castaneda E, Brummer E, Perlman AM, McEwen JG, Stevens DA. A culture medium for Paracoccidioides brasiliensis with high plating efficiency, and the effect of siderophores. J Med Vet Mycol 1988; 26:351–358
    [Google Scholar]
  11. Gray PW, Leung DW, Pennica D. Expression of human immune interferon cDNA in E. coli and monkey cells. Nature 1982; 295:503–508
    [Google Scholar]
  12. Musson RA, Shafran H, Henson PM. Intracellular levels and stimulated release of lysosomal enzymes from human peripheral blood monocytes and monocyte-derived macrophages. J Reticuloendothel Soc 1980; 28:249–264
    [Google Scholar]
  13. Brummer E, Kurita N, Yoshida S, Nishimura K, Miyaji M. Killing of Histoplasma capsulatum by γ-interferon activated human monocyte-derived macrophages: evidence for a superoxide anion-dependent mechanism. J Med Microbiol 1991; 35:29–34
    [Google Scholar]
  14. Brummer E, Kurita N, Yoshida S, Nishimura K, Miyaji M. Fungistatic activity of human neutrophils against Histoplasma capsulatum: correlation with phagocytosis. J Infect Dis 1991; 164:158–162
    [Google Scholar]
  15. Brummer E, Stevens DA. Opposite effects of human monocytes, macrophages, and polymorphonuclear neutrophils on replication of Blastomyces dermatitidis in vitro. Infect Immun 1982; 36:297–303
    [Google Scholar]
  16. Catterall JR, Black CM, Leventhal JP, Rizk NW, Wachtel JS, Remington JS. Nonoxidative microbicidal activity in normal human alveolar and peritoneal macrophages. Infect Immun 1987; 55:1635–1640
    [Google Scholar]
  17. Nguyen B-YT, Peterson PK, Verbrugh HA, Quie PG, Hoidal JR. Differences in phagocytosis and killing by alveolar macrophages from humans, rabbits, rats, and hamsters. Infect Immun 1982; 36:504–509
    [Google Scholar]
  18. Byrd TF, Horwitz MA. Interferon gamma-activated human monocytes downregulate transferrin receptors and inhibit the intracellular multiplication of Legionella pneumophila by limiting the availability of iron. J Clin Invest 1989; 83:1457–1465
    [Google Scholar]
  19. Hamilton TA, Gray PW, Adams DO. Expression of the transferrin receptor on murine peritoneal macrophages is modulated by in vitro treatment with interferon-gamma. Cell Immunol 1984; 89:478–488
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-40-3-159
Loading
/content/journal/jmm/10.1099/00222615-40-3-159
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error