The interaction of human monocytes or monocyte-derived macrophages and yeast-form was studied . Yeast cells were readily ingested by adherent monocytes or macrophages. Multiplication of , measured by growth as colony forming units (cfu) on a supplemented medium with good plating efficiency, was greater in monocyte co-cultures compared to the number of cfu obtained from complete tissue-culture medium (CTCM). Multiplication increased with time in macrophage co-cultures, e.g., from two-six-fold in 24 h to nine-fold in 72 h. Microscopic observations indicated that ingested yeast cells multiplied inside macrophages. When monocytes were treated with supernate cytokines (CK) from concanavalin-A-stimulated mononuclear cells, then co-cultured with , multiplication was significantly inhibited compared with control monocyte co-cultures. Treatment of macrophages—derived from monocytes by culture in for 3 days—for a further 3 days with CK resulted in maximal inhibition of multiplication over the subsequent 72 h. Similarly, when monocyte-derived macrophages (after culture for 7 days) were treated for 3 days with recombinant human γ-interferon (IFN; 300U/ml) or CK they restricted multiplication of by 65% and 95%, respectively, compared with control macrophages. Antibody to IFN abrogated the effect of IFN or CK treatment. These findings show that ingested can multiply in human monocytes or macrophages and that this multiplication can be restricted by activated monocytes or macrophages.


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