1887

Abstract

Summary

During the 1980s the emergence of multiple antibiotic-resistant strains of posed new problems for infection control worldwide, many of which still remain. In investigations of outbreaks of infection, the laboratory has a key role in identifying infected or colonised patients and staff. The rapid isolation and accurate identification of the causative organisms are essential for the implementation of appropriate control measures. Speed and accuracy in identification, by colonial morphology, is often difficult to achieve in the presence of a mixed population of commensal bacteria.

To this end, the sensitivity of three media for the isolation of methicillin-resistant (MRSA) from simulated clinical specimens was compared. Initial colonial recognition of MRSA was enhanced on methicillin-milk agar when compared with that on other media.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-40-2-98
1994-02-01
2022-10-01
Loading full text...

Full text loading...

/deliver/fulltext/jmm/40/2/medmicro-40-2-98.html?itemId=/content/journal/jmm/10.1099/00222615-40-2-98&mimeType=html&fmt=ahah

References

  1. Archer GL, Penneil E. Detection of methicillin resistance in staphylococci by using a DNA probe. Antimicrob Agents Chemother 1990; 34:1720–1724
    [Google Scholar]
  2. Murakami K, Minamide W, Wada K, Nakamura E, Teraoka H, Watanabe S. Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. J Clin Microbiol 1991; 29:2240–2244
    [Google Scholar]
  3. Harrington CR, O’Hara DM, Reynolds PE. Characterisation of a monoclonal antibody and its use in the immunoaffinity purification of penicillin-binding protein 2’ of methicillin-resistant Staphylococcus aureus . FEMS Microbiol Lett 1989; 65:143–147
    [Google Scholar]
  4. Report of a Combined Working Party of the Hospital Infection Society and British Society for Antimicrobial Chemotherapy. Revised guidelines for the control of epidemic methicillin-resistant Staphylococcus aureus . J Hosp Infect 1990; 16:351–377
    [Google Scholar]
  5. Christie R, Keogh EV. Physiological and serological characteristics of staphylococci of human origin. J Pathol Bacteriol 1940; 51:189–197
    [Google Scholar]
  6. Willis AT, O’Connor JJ, Smith JA. Colonial pigmentation of Staphylococcus aureus . J Pathol Bacteriol 1966; 92:97–106
    [Google Scholar]
  7. Chapman GH. An improved Stone medium for the isolation and testing of food-poisoning staphylococci. Food Research 1948; 13:100–105
    [Google Scholar]
  8. Garrod LP, Lambert HP, O’Grady F. Antibiotics and chemotherapy, 5. Edinburgh: Churchill Livingstone; 1981474
    [Google Scholar]
  9. Goldschmidt MC, Fung DY, Grant R, White J, Brown T. New aniline blue dye medium for rapid identification and isolation of Candida albicans . J Clin Microbiol 1991; 29:1095–1099
    [Google Scholar]
  10. Report of the Working Party on Antibiotic Sensitivity Testing of the British Society for Antimicrobial Chemotherapy. A guide to sensitivity testing. J Antimicrob Chemother 1991; 27:1–50
    [Google Scholar]
  11. Flournoy DJ, Wongpradit S, Silberg SL. Screening media for detection of methicillin-resistant Staphylococcus aureus from non-sterile body sites. Med Microbiol Immunol 1990; 179:25–30
    [Google Scholar]
  12. Cookson B, Peters B, Webster M, Phillips I, Rahman M, Noble W. Staff carriage of epidemic methicillin-resistant Staphylococcus aureus . J Clin Microbiol 1989; 27:1471–1476
    [Google Scholar]
  13. Lally RT, Ederer MN, Woolfrey BF. Evaluation of mannitol salt agar with oxacillin as a screening medium for methicillin-resistant Staphylococcus aureus . J Clin Microbiol 1985; 22:501–504
    [Google Scholar]
  14. Cruickshank R, Duguid JP, Swain RHA. Medical microbiology, a guide to the laboratory diagnosis and control of infection, 11th. Edinburgh: Churchill Livingstone; 1965748
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-40-2-98
Loading
/content/journal/jmm/10.1099/00222615-40-2-98
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error