1887

Abstract

Summary

Seventy-one strains isolated from diarrhoeic weaned rabbits from different areas of France were tested for the presence of DNA sequences specific for the EPEC, EHEC, DAEC and EAggEC strains and 16 of them were tested for pathogenicity in animal experiments. High pathogenicity was observed only with strains unable to ferment rhamnose. DNA from all 55 rhamnose-negative O103,O26 and rough strains hybridised with the probe. Similar hybridisation was obtained with six non-pathogenic rhamnose-positive strains belonging to serogroups O128 and O132. No hybridisation was observed with the other probes. This is the first report of the presence of sequences in genomic DNA of non-pathogenic strains.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-40-2-90
1994-02-01
2024-07-23
Loading full text...

Full text loading...

/deliver/fulltext/jmm/40/2/medmicro-40-2-90.html?itemId=/content/journal/jmm/10.1099/00222615-40-2-90&mimeType=html&fmt=ahah

References

  1. Peeters JE, Geeroms R, Orskov F. Biotype, serotype, and pathogenicity of attaching and effacing enteropathogenic Escherichia coli strains isolated from diarrheic commercial rabbits. Infect Immun 1988; 56:1442–1448
    [Google Scholar]
  2. Camguilhem R, Milon A. Biotypes and O serogroups of Escherichia coli involved in intestinal infections of weaned rabbits: clues to diagnosis of pathogenic strains. J Clin Microbiol 1989; 27:743–747
    [Google Scholar]
  3. Cantey JR, Blake RK. Diarrhea due to Escherichia coli in the rabbit: a novel mechanism. J Infect Dis 1977; 135:454–462
    [Google Scholar]
  4. Milon A, Esslinger J, Camguilhem R. Adhesion of Escherichia coli strains isolated from diarrheic weaned rabbits to intestinal villi and Hela cells. Infect Immun 1990; 58:2690–2695
    [Google Scholar]
  5. Licois D, Reynaud A, Federighi M, Gaillard-Martinie B, Guillot JF, Joly B. Scanning and transmission electron microscopic study of adherence of Escherichia coli O103 enteropathogenic and/or enterohemorrhagic strain GV in enteric infection in rabbits. Infect Immun 1991; 59:3796–3800
    [Google Scholar]
  6. Moon HW, Whipp SC, Argenzio RA, Levine MM, Giannella RA. Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines. Infect Immun 1983; 41:1340–1351
    [Google Scholar]
  7. Francis DH, Collins JE, Duimstra JR. Infection of gnotobiotic pigs with an Escherichia coli O157:H7 strain associated with an outbreak of hemorrhagic colitis. Infect Immun 1986; 51:953–956
    [Google Scholar]
  8. Jerse AE, Yu J, Tall BD, Kaper JB. A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Nati Acad SciUSA 1990; 87:7839–7843
    [Google Scholar]
  9. Donnenberg MS, Kaper JB. Enteropathogenic Escherichia coli . Infect Immun 1992; 60:3953–3961
    [Google Scholar]
  10. Jerse AE, Kaper JB. The eae gene of enteropathogenic Escherichia coli encodes a 94-kilodalton membrane protein, the expression of which is influenced by the EAF plasmid. Infect Immun 1991; 59:4302–4309
    [Google Scholar]
  11. Nataro JP, Baldini MM, Kaper JB, Black RE, Bravo N, Levine MM. Detection of an adherence factor of enteropathogenic Escherichia coli with a DNA probe. J Infect Dis 1985; 152:560–565
    [Google Scholar]
  12. Jerse AE, Gicquelais KG, Kaper JB. Plasmid and chromosomal elements involved in the pathogenesis of attaching and effacing Escherichia coli . Infect Immun 1991; 59:3869–3875
    [Google Scholar]
  13. Newland JW, Neill RJ. DNA probes for Shiga-like toxins I and II and for toxin-converting bacteriophages. J Clin Microbiol 1988; 26:1292–1297
    [Google Scholar]
  14. Levine MM, Xu J, Kaper JB. A DNA probe to identify enterohemorrhagic Escherichia coli of O157: H7 and other serotypes that cause hemorrhagic colitis and hemolytic uremic syndrome. J Infect Dis 1987; 156:175–182
    [Google Scholar]
  15. Bilge SS, Clausen CR, Lau W, Moseley SL. sMolecular characterization of a fimbrial adhesin, F1845, mediating diffuse adherence of diarrhea-associated Escherichia coli to Hep-2 cells. J Bacteriol 1989; 171:4281–4289
    [Google Scholar]
  16. Baudry B, Savarino SJ, Vial P, Kaper JB, Levine MM. A sensitive and specific DNA probe to identify entero-aggregative Escherichia coli, a recently discovered diarrheal pathogen. J Infect Dis 1990; 161:1249–1251
    [Google Scholar]
  17. Milon A, Esslinger J, Camguilhem R. Oral vaccination of weaned rabbits against enteropathogenic Escherichia coli-like E. coli O103 infection: use of heterologous strains harboring lipopolysaccharide or adhesin of pathogenic strains. Infect Immun 1992; 60:2702–2709
    [Google Scholar]
  18. Finkelstein M, Rownd RH. A rapid method for extracting DNA from agarose gels. Plasmid 1978; 1:557–562
    [Google Scholar]
  19. Takahashi S, Nagano Y. Rapid procedure for isolation of plasmid DNA and application to epidemiological analysis. J Clin Microbiol 1984; 20:608–613
    [Google Scholar]
  20. Wilson K. Preparation of genomic DNA. Ausubel FM, Brent R, Kingston RE. (eds) Current protocols in molecular biology 1 New York: 19872.4.1.–2.4.2.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-40-2-90
Loading
/content/journal/jmm/10.1099/00222615-40-2-90
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error