The enteropathogenicity of Aeromonas strains that showed mannose-resistant adhesion to INT407 cells was evaluated by infecting Caco-2 cells and observing them by light and electronmicroscopy. Five of six strains adhered in large numbers to Caco-2 cells in the presence of mannose and caused cytopathic effects. Two strains of Aeromonas spp. seemed to invade Caco-2 cells, as membrane-bound bacteria were seen within the cytoplasm of these cells; however, staining by acridine orange-crystal violet appeared to show intracellular fluorescent bacteria in three strains. Fimbriae did not appear to play an important role in adhesion because fimbrial structures were not seen by transmission electronmicroscopy. Adhesion of four strains was inhibited by the addition of l-fucose. The strains were negative in the fluorescence actin staining test, which in enteropathogenic Escherichia coli strains correlates with the ability to attach and efface intestinal microvilli. The DNA of the Aeromonas strains did not hybridise with the E. coli eae and ipaB probes, associated with attaching and effacing ability and invasion, respectively. These results give support to the enteropathogenicity of adhesive strains of Aeromonas spp., although the mechanisms of adhesion, and possibly invasion, remain to be elucidated.
JandaJM.
Recent advances in the study of the taxonomy, pathogenicity, and infectious syndromes associated with the genus Aeromonas. Clin Microbiol Rev1991; 4:397–410
CallisterSM,
AggerWA.
Enumeration and characterization of Aeromonas hydrophila and Aeromonas caviae isolated from grocery store produce. Appl Environ Microbiol1987; 53:249–253
PalumboSA,
MaxinoF,
WilliamsAC,
BuchananRL,
ThayerDW.
Starch-ampicillin agar for the quantitative detection of Aeromonas hydrophila. Appl Environ Microbiol1985; 50:1027–1030
StelmaGN,
JohnsonCH,
SpauldingP.
Evidence for the direct involvement of β-hemolysin in Aeromonas hydrophila enteropathogenicity. Curr Microbiol1986; 14:71–77
MorganDR,
JohnsonPC,
DuPontHL,
SatterwhiteTK,
WoodLV.
Lack of correlation between known virulence properties of Aeromonas hydrophila and enteropathogenicity for humans. Infect Immun1985; 50:62–65
FréterR.
Association of enteropathogenic bacteria with the mucosa of the small intestine: mechanisms and pathogenic implications. OuchterlonyO,
HolmgrenJ,
CraigJ,
BaruaD.
Cholera and related diarrheas Basel: Karger; 1978155–170
NishikawaY,
KimuraT,
KishiT.
Mannose-resistant adhesion of motile Aeromonas to INT407 cells and the differences among isolates from humans, food and water. Epidemiol Infect1991; 107:171–179
PintoM,
Robine-LeonS,
AppayMD.
Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol Cell1983; 47:323–330
Darfeuille-MichaudA,
AubelD,
ChauvièreG.
Adhesion of enterotoxigenic Escherichia coli to the human colon carcinoma cell line Caco-2 in culture. Infect Immun1990; 58:893–902
EverestPH,
GoossensH,
ButzlerJ-P.
Differentiated Caco-2 cells as a model for enteric invasion by Campylobacter jejuni and C. coli. J Med Microbiol1992; 37:319–325
KerneisS,
BilgeSS,
FourelV,
ChauvièreG,
CoconnierM-H,
ServinAL.
Use of purified F1845 fimbrial adhesin to study localization and expression of receptors for diffusely adhering Escherichia coli during enterocytic differentiation of human colon carcinoma cell lines HT-29 and Caco-2 in culture. Infect Immun1991; 59:4013–4018
MounierJ,
VasselonT,
HellioR,
LesourdM,
SansonettiPJ.
Shigella flexneri enters human colonic Caco-2 epithelial cells through the basolateral pole. Infect Immun1992; 60:237–248
RigothierMC,
CoconnierMH,
ServinAL,
GayralP.
A new in vitro model of Entamoeba histolytica adhesion, using the human colon carcinoma cell line Caco-2 : scanning electron microscopic study. Infect Immun1991; 59:4142–4146
PanigrahiP,
TallBD,
RussellRG,
DetollaLJ,
MorrisJG.
Development of an in vitro model for study of non-O1 Vibrio cholerae virulence using Caco-2 cells. Infect Immun1990; 58:3415–3424
JerseAE,
YuJ,
TallBD,
KaperJB.
A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Nati Acad Sci USA1990; 87:7839–7843
BaudryB,
MaurelliAT,
ClercP,
SadoffJC,
SansonettiPJ.
Localization of plasmid loci necessary for the entry of Shigella flexneri into HeLa cells, and characterization of one locus encoding four immunogenic polypeptides. J Gen Microbiol1987; 133:3403–3413
KnuttonS,
BaldwinT,
WilliamsPH,
McNeishAS.
Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli. Infect Immun1989; 57:1290–1298
KnuttonS,
LloydDR,
McNeishAS.
Identification of a new fimbrial structure in enterotoxigenic Escherichia coli (ETEC) serotype O148:H28 which adheres to human intestinal mucosa: a potentially new human ETEC colonization factor. Infect Immun1987; 55:86–92
ChartH,
ScotlandSM,
WillshawGA,
RoweB.
HEp-2 adhesion and the expression of a 94 KDa outer-membrane protein by strains of Escherichia coli belonging to entero-pathogenic serogroups. J Gen Microbiol1988; 134:1315–1321
OwenP.
Outer membrane proteins: their role in virulence. OwenP,
FosterTJ.
Immunochemical and molecular genetic analysis of bacterial pathogens Amsterdam: Elsevier; 198833–37