RT Journal Article SR Electronic(1) A1 de Graaf-Miltenburg, Lia A. M. A1 Van Vliet, Karin E. A1 Ten Hagen, T. L. M. A1 Verhoef, J. A1 van Strijp, J. A. G.YR 1994 T1 The role of HSV-induced Fc- and C3b(i)-receptors in bacterial adherence JF Journal of Medical Microbiology, VO 40 IS 1 SP 48 OP 54 DO https://doi.org/10.1099/00222615-40-1-48 PB Microbiology Society, SN 1473-5644, AB Summary. Herpes simplex virus type-1 (HSV-1) induces Fc- and C3b(i)-receptors on infected cells. The role of these receptors in baceterial superinfection was studied by comparing the adherence of non-opsonised and opsonised bacteria to HSV-infected and non-infected HEp-2 cells. A flow cytometric adherence assay, based on the fluorescent quantitation of FITC-labelled bacteria, was developed. Opsonisation of Staphylococcus epidermidis with human serum, resulted in a marked increase in adherence to HSV-infected cells and revealed a role for C3b(i)R- and FcR-mediated adhesion. However, the enhanced adherence never exceeded the level of attachment to non-infected cells. Increased adherence of other pathogenic bacteria, including Escherichia coli, Streptococcus pneumoniae, Haemophilus influenzae and Pseudomonas aeruginosa was not observed, indicating that the HSV-receptors play a minor role in secondary infections. Bacterial adhesion factors such as the fimbriae of E. coli played a more dominant role in the adherence of bacteria to HSV-infected cells., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-40-1-48