RT Journal Article SR Electronic(1) A1 Said, Bengü A1 Scotland, Sylvia M. A1 Rowe, B.YR 1994 T1 The use of gene probes, immunoassays and tissue culture for the detection of toxin in Vibrio cholerae non-O1 JF Journal of Medical Microbiology, VO 40 IS 1 SP 31 OP 36 DO https://doi.org/10.1099/00222615-40-1-31 PB Microbiology Society, SN 1473-5644, AB Summary. Vibrio cholerae non-O1 strains were screened for the presence of cholera enterotoxin (CT) genes by means of digoxigenin-labelled polynucleotide CTA and CTB probes. In-vitro production of CT was investigated by the Y1 mouse adrenal cell assay, enzyme-linked immunosorbent assay (ELISA) and a commercial, reversed passive latex agglutination (RPLA) kit. Only two (0·25%) of 790 strains tested gave positive results with the CTA and CTB probes. The production of other bacterial cytotoxin(s) made it impossible to use the characteristic cell-rounding effect on Y1 cells for the detection of CT. CT production by the probe-positive strains was confirmed by the immunoassays. Two hundred and fifty-two of the 788 probe-negative strains were tested by both cell assay and immunoassays. Of these, 90% produced cytotoxin(s) in the cell assay. In addition, 37% gave positive results in CT-ELISA, but negative results with LT-ELISA and VET-RPLA. These results indicate the presumed presence of a toxin in V. cholerae non-O1 that is able to bind GM1 and react with antisera to CT, but which is not identical to CT., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/00222615-40-1-31